BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_M11544 1 BBa_M11544 Fe (iron) Promoter (point mutations) 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z Part BBa_I765000 This promoter is a mutated version of part BBa_I765000 at all of its PstI restriction sites. false false _572_ 0 6772 9 Not in stock false There were several PstI restriction sites that had to be mutated. The six nucleotide sequence for PstI is ctgcag. We mutated it so that the sequence became ctacag, keeping the appropriate amino acid still in place, but getting rid of the potential for PstI to cut it. false Alyssa Calder BBa_E0020 1 ecfp engineered cyan fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M11543 1 BBa_M11543 Fe Promoter with CFP 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z BBa_M11544 and BBa_E0020 An iron promoter, regulated by the presence of iron, with CFP. Allows for detection of iron in a substance. false false _572_ 0 6772 9 Not in stock false none false Alyssa Calder component2068262 1 BBa_E0020 component2068263 1 BBa_B0010 component2068259 1 BBa_M11544 component2068265 1 BBa_B0012 component2068261 1 BBa_B0034 annotation2068263 1 BBa_B0010 range2068263 1 1802 1881 annotation2068265 1 BBa_B0012 range2068265 1 1890 1930 annotation2068259 1 BBa_M11544 range2068259 1 1 1044 annotation2068262 1 BBa_E0020 range2068262 1 1071 1793 annotation2068261 1 BBa_B0034 range2068261 1 1053 1064 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_E0020_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_M11544_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctacaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctacaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctacaggcgatgaagcctgacctacagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaa BBa_B0034_sequence 1 aaagaggagaaa BBa_M11543_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctacaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctacaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctacaggcgatgaagcctgacctacagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z