BBa_M11544 1 BBa_M11544 Fe (iron) Promoter (point mutations) 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z Part BBa_I765000 This promoter is a mutated version of part BBa_I765000 at all of its PstI restriction sites. false false _572_ 0 6772 9 Not in stock false There were several PstI restriction sites that had to be mutated. The six nucleotide sequence for PstI is ctgcag. We mutated it so that the sequence became ctacag, keeping the appropriate amino acid still in place, but getting rid of the potential for PstI to cut it. false Alyssa Calder BBa_M11544_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctacaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctacaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctacaggcgatgaagcctgacctacagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z