BBa_K208000 1 BBa_K208000 GFP 2009-10-07T11:00:00Z 2015-05-08T01:11:24Z plasmid GFP false false _310_ 0 2425 303 It's complicated true Mutate PstI sites false Elisabeth Linton annotation2062216 1 Start range2062216 1 1 3 annotation2062217 1 GFP Coding Region range2062217 1 1 720 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_M11561 1 BBa_M11561 CAD1 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z S. cerevisiae Cadmium induced transcriptional activator false false _572_ 0 5417 9 Not in stock false later - false Brad Henrie annotation2067852 1 Start range2067852 1 1 3 annotation2067853 1 Stop range2067853 1 1228 1230 annotation2067854 1 Coding region range2067854 1 1 1228 BBa_M11562 1 BBa_M11562 p(CAD1) 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z Saccharomyces cerevisiae genomic sequence Promoter region corresponding to CAD1 false false _572_ 0 5417 9 Not in stock false Altered genetic sequence for improved CAD1 binding false Brad Henrie annotation2067855 1 CAD1 Binding site range2067855 1 13 22 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M11551 1 BBa_M11551 Cd induced GFP reporter 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z This part was constructed from other well used biobricks. Cadmium induced biosensor using GFP as the reporter false false _572_ 0 5417 9 Not in stock false None false Brad Henrie component2067910 1 BBa_B0034 component2067918 1 BBa_B0010 component2067914 1 BBa_M11561 component2067917 1 BBa_K208000 component2067908 1 BBa_M11562 component2067920 1 BBa_B0012 annotation2067908 1 BBa_M11562 range2067908 1 1 51 annotation2067914 1 BBa_M11561 range2067914 1 78 1307 annotation2067910 1 BBa_B0034 range2067910 1 60 71 annotation2067918 1 BBa_B0010 range2067918 1 2042 2121 annotation2067920 1 BBa_B0012 range2067920 1 2130 2170 annotation2067917 1 BBa_K208000 range2067917 1 1314 2033 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M11561_sequence 1 atgggcaatatccttcggaaaggtcagcaaatatatttagcaggtgacatgaagaagcaaatgttgctaaataaagatggaacacctaagaggaaggtgggcagaccaggcagaaaaaggattgactctgaagctaagagtaggaggactgcccagaatagggcagctcaacgagcgttccgagataggaaagaagccaaaatgaagagtttgcaagagagggtagagttactagaacagaaagatgcgcagaataagactaccacggactttttactatgttctttaaaaagtttactgtcggaaattacaaaatatagagctaagatttctgatgatgaaagaatattagccttcctcgatgatctgcaagaacaacagaaaagggaaaacgaaaaaggaacaagtacagcagttagcaaggctgcaaaggaattgccatcgcctaattcagatgaaaacatgactgtgaacacaagtatagaagtacagccgcacactcaagagaatgagaaagttatgtggaacataggctcatggaacgctcccagtttaaccaattcgtgggattctccccccggaaatcgaacaggtgccgttaccatcggtgacgaaagtattaatggtagtgaaatgccagatttcagtctcgatcttgtctccaatgatagacagactggtcaagaagctttagattacgacattcataactactttcctcagcactctgaacgcctgaccgctgaaaaaatagatacgtcagcatgtcaatgtgaaattgaccaaaagtatcttccatacgagacagaagatgatactttattccccagcgtgcttccccttgctgtagggagccagtgtaataatatttgcaaccgcaagtgtatcgggaccaaaccatgttcaaataaggagatcaaatgcgacttaataacaagccacctgttgaatcagaaatctctagcttcggtgcttccggtggctgcttctcatactaaaacaattcgaacccaatctgaagcaattgaacacattagcagcgccatatcgaatggaaaagcgtcttgctaccacattctcgaagagaactcctccctaccaaaatattcatcgttggacatagatgatttatgcagcgaattaataatcaaggcaaaatgtacagatgactgcaaaatagtagtcaaagctcgcgacttacagagtgctctggttagacagctcctgtag BBa_M11551_sequence 1 tattacatgctcattactaatcttatgtatactcatgtaatgtttatatgctactagagaaagaggagaaatactagatgggcaatatccttcggaaaggtcagcaaatatatttagcaggtgacatgaagaagcaaatgttgctaaataaagatggaacacctaagaggaaggtgggcagaccaggcagaaaaaggattgactctgaagctaagagtaggaggactgcccagaatagggcagctcaacgagcgttccgagataggaaagaagccaaaatgaagagtttgcaagagagggtagagttactagaacagaaagatgcgcagaataagactaccacggactttttactatgttctttaaaaagtttactgtcggaaattacaaaatatagagctaagatttctgatgatgaaagaatattagccttcctcgatgatctgcaagaacaacagaaaagggaaaacgaaaaaggaacaagtacagcagttagcaaggctgcaaaggaattgccatcgcctaattcagatgaaaacatgactgtgaacacaagtatagaagtacagccgcacactcaagagaatgagaaagttatgtggaacataggctcatggaacgctcccagtttaaccaattcgtgggattctccccccggaaatcgaacaggtgccgttaccatcggtgacgaaagtattaatggtagtgaaatgccagatttcagtctcgatcttgtctccaatgatagacagactggtcaagaagctttagattacgacattcataactactttcctcagcactctgaacgcctgaccgctgaaaaaatagatacgtcagcatgtcaatgtgaaattgaccaaaagtatcttccatacgagacagaagatgatactttattccccagcgtgcttccccttgctgtagggagccagtgtaataatatttgcaaccgcaagtgtatcgggaccaaaccatgttcaaataaggagatcaaatgcgacttaataacaagccacctgttgaatcagaaatctctagcttcggtgcttccggtggctgcttctcatactaaaacaattcgaacccaatctgaagcaattgaacacattagcagcgccatatcgaatggaaaagcgtcttgctaccacattctcgaagagaactcctccctaccaaaatattcatcgttggacatagatgatttatgcagcgaattaataatcaaggcaaaatgtacagatgactgcaaaatagtagtcaaagctcgcgacttacagagtgctctggttagacagctcctgtagtactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K208000_sequence 1 atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa BBa_M11562_sequence 1 tattacatgctcattactaatcttatgtatactcatgtaatgtttatatgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z