BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_M11561 1 BBa_M11561 CAD1 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z S. cerevisiae Cadmium induced transcriptional activator false false _572_ 0 5417 9 Not in stock false later - false Brad Henrie annotation2067853 1 Stop range2067853 1 1228 1230 annotation2067852 1 Start range2067852 1 1 3 annotation2067854 1 Coding region range2067854 1 1 1228 BBa_M11552 1 BBa_M11552 Cd induced RFP reporter 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z other parts Cadmium induced biosensor using RFP as the reporter false false _572_ 0 5417 9 Not in stock false None false Brad Henrie component2263431 1 BBa_B0034 component2263435 1 BBa_M11561 component2263439 1 BBa_B0010 component2263441 1 BBa_B0012 component2263438 1 BBa_E1010 component2263429 1 BBa_M11562 annotation2263438 1 BBa_E1010 range2263438 1 1314 2019 annotation2263439 1 BBa_B0010 range2263439 1 2028 2107 annotation2263435 1 BBa_M11561 range2263435 1 78 1307 annotation2263431 1 BBa_B0034 range2263431 1 60 71 annotation2263429 1 BBa_M11562 range2263429 1 1 51 annotation2263441 1 BBa_B0012 range2263441 1 2116 2156 BBa_M11562 1 BBa_M11562 p(CAD1) 2010-04-20T11:00:00Z 2015-05-08T01:13:54Z Saccharomyces cerevisiae genomic sequence Promoter region corresponding to CAD1 false false _572_ 0 5417 9 Not in stock false Altered genetic sequence for improved CAD1 binding false Brad Henrie annotation2067855 1 CAD1 Binding site range2067855 1 13 22 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M11561_sequence 1 atgggcaatatccttcggaaaggtcagcaaatatatttagcaggtgacatgaagaagcaaatgttgctaaataaagatggaacacctaagaggaaggtgggcagaccaggcagaaaaaggattgactctgaagctaagagtaggaggactgcccagaatagggcagctcaacgagcgttccgagataggaaagaagccaaaatgaagagtttgcaagagagggtagagttactagaacagaaagatgcgcagaataagactaccacggactttttactatgttctttaaaaagtttactgtcggaaattacaaaatatagagctaagatttctgatgatgaaagaatattagccttcctcgatgatctgcaagaacaacagaaaagggaaaacgaaaaaggaacaagtacagcagttagcaaggctgcaaaggaattgccatcgcctaattcagatgaaaacatgactgtgaacacaagtatagaagtacagccgcacactcaagagaatgagaaagttatgtggaacataggctcatggaacgctcccagtttaaccaattcgtgggattctccccccggaaatcgaacaggtgccgttaccatcggtgacgaaagtattaatggtagtgaaatgccagatttcagtctcgatcttgtctccaatgatagacagactggtcaagaagctttagattacgacattcataactactttcctcagcactctgaacgcctgaccgctgaaaaaatagatacgtcagcatgtcaatgtgaaattgaccaaaagtatcttccatacgagacagaagatgatactttattccccagcgtgcttccccttgctgtagggagccagtgtaataatatttgcaaccgcaagtgtatcgggaccaaaccatgttcaaataaggagatcaaatgcgacttaataacaagccacctgttgaatcagaaatctctagcttcggtgcttccggtggctgcttctcatactaaaacaattcgaacccaatctgaagcaattgaacacattagcagcgccatatcgaatggaaaagcgtcttgctaccacattctcgaagagaactcctccctaccaaaatattcatcgttggacatagatgatttatgcagcgaattaataatcaaggcaaaatgtacagatgactgcaaaatagtagtcaaagctcgcgacttacagagtgctctggttagacagctcctgtag BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_M11562_sequence 1 tattacatgctcattactaatcttatgtatactcatgtaatgtttatatgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M11552_sequence 1 tattacatgctcattactaatcttatgtatactcatgtaatgtttatatgctactagagaaagaggagaaatactagatgggcaatatccttcggaaaggtcagcaaatatatttagcaggtgacatgaagaagcaaatgttgctaaataaagatggaacacctaagaggaaggtgggcagaccaggcagaaaaaggattgactctgaagctaagagtaggaggactgcccagaatagggcagctcaacgagcgttccgagataggaaagaagccaaaatgaagagtttgcaagagagggtagagttactagaacagaaagatgcgcagaataagactaccacggactttttactatgttctttaaaaagtttactgtcggaaattacaaaatatagagctaagatttctgatgatgaaagaatattagccttcctcgatgatctgcaagaacaacagaaaagggaaaacgaaaaaggaacaagtacagcagttagcaaggctgcaaaggaattgccatcgcctaattcagatgaaaacatgactgtgaacacaagtatagaagtacagccgcacactcaagagaatgagaaagttatgtggaacataggctcatggaacgctcccagtttaaccaattcgtgggattctccccccggaaatcgaacaggtgccgttaccatcggtgacgaaagtattaatggtagtgaaatgccagatttcagtctcgatcttgtctccaatgatagacagactggtcaagaagctttagattacgacattcataactactttcctcagcactctgaacgcctgaccgctgaaaaaatagatacgtcagcatgtcaatgtgaaattgaccaaaagtatcttccatacgagacagaagatgatactttattccccagcgtgcttccccttgctgtagggagccagtgtaataatatttgcaaccgcaagtgtatcgggaccaaaccatgttcaaataaggagatcaaatgcgacttaataacaagccacctgttgaatcagaaatctctagcttcggtgcttccggtggctgcttctcatactaaaacaattcgaacccaatctgaagcaattgaacacattagcagcgccatatcgaatggaaaagcgtcttgctaccacattctcgaagagaactcctccctaccaaaatattcatcgttggacatagatgatttatgcagcgaattaataatcaaggcaaaatgtacagatgactgcaaaatagtagtcaaagctcgcgacttacagagtgctctggttagacagctcctgtagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z