LOCUS BBa_M1193 4363 bp DNA linear 26-Dec-2024 DEFINITION Gabi's part in GFP assay ACCESSION BBa_M1193 VERSION BBa_M1193.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1136..1138 /label=Beginning of LacI_mutant ORF promoter 62..2215 /label=BBa_M1632 promoter 2224..2253 /label=BBa_K233004 misc_feature 2260..4275 /label=BBa_M1635 stem_loop 4295..4338 /label=stem_loop misc_feature 4284..4363 /label=BBa_B0010 terminator 4284..4363 /label=BBa_B0010 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgaaaatc gccatattag gggcaggctg ctaccggaca cacgcagcag caggtataac 121 aaattttatg cgggcgtgtg aggtagctaa agaggtcgga aagcctgaga ttgcactaac 181 gcattccagt atcacgtatg gggccgagct gctccatctc gtcccagatg taaaggaggt 241 aattgttagc gatccgtgtt ttgcagaaga gcccggcctg gtcgtcattg atgagtttga 301 cccaaaagag gtgatggagg cccatcttag cggtaatccg gaaagtatta tgcccaaaat 361 ccgggaagtc gtgaaggcta aagcgaaaga gttaccaaaa ccccccaagg catgcatcca 421 tctggtacat ccggaagacg tggggctgaa agttacgagc gatgatcgcg aggccgttga 481 gggagccgac atcgtgatca catggttacc gaaggggaac aagcagcccg atataattaa 541 aaaatttgcc gatgccatac cggaaggagc gatcgttacc cacgcatgta cgatcccaac 601 gaccaaattc gcaaagattt tcaaagatct aggacgtgaa gatctgaaca ttacatcata 661 ccacccgggt tgcgtgcctg agatgaaggg gcaagtatac attgcggaag gatacgcttc 721 tgaagaagct gtgaacaaac tgtacgaaat tggtaaaatc gcgcggggga aagcgttcaa 781 aatgcccgca aacctgatcg gtccggtttg cgacatgtgt tcagctgtca ccgccacggt 841 atatgcggga cttttagcat acagagatgc ggttaccaaa attttaggag caccagctga 901 cttcgcgcaa atgatggccg atgaggccct gacccaaatc cataatctga tgaaagaaaa 961 aggcattgca aatatggagg aagctttgga cccggccgct ttgctgggta ccgccgacag 1021 tatgtgcttt ggcccgctgg cagagatctt acccactgct ctgaaagtgc tggagaaaca 1081 caaagttgtg gaagaggagg gtaaaaccaa gtgcgagata atgtcgcaga aagaaatgaa 1141 acctgtgact ctgtatgatg ttgcggagta tgcaggtgtc tcgtaccaga ccgtgagccg 1201 cgttgttaac caagctagtc atgtcagcgc gaaaacgcgt gagaaagtgg aagctgcaat 1261 ggcggaattg aactacattc caaatcgtgt cgcacagcaa ctagcgggca aacaatctct 1321 tctgataggc gtagctacga gttctctggc attgcaccat cactcccaat gtgttgcagc 1381 gataaagtca cgtgcagatc aattgggtgc gtcggtggtc gtgtctatgg tggaaagatc 1441 aggtgtggaa gcatgcaaag cagcggttca taatctgctg gcacaacgtg tatcaggact 1501 actgattaac tatccccttg acgatcaaga tgcgacggcc gttgaagccg catgcgctaa 1561 tgtccctgcc cttttcttag acgttagtga tcaaactccg atcaattcaa tcatttttag 1621 ccatgaggat ggcactcggc ttggcgtaga gcacttagta gcgttgggac atcaacagat 1681 tgctcttttg gcgggcccat taagcagtgt gtcagcgcgt ttgcgactag ccggctggca 1741 caaatatttg acccgcaacc aaatccagcc gattgctgag cgggagggcg attggtcggc 1801 aatgtctggc tttcagcaaa cgatgcaaat gctgaatgaa ggaatcgtcc caactgcaat 1861 gctggtggct aacgatcaga tggccttagg cgcgatgcgt gcaatcaccg agtctggact 1921 gcgggtggga gcagatatat cggtcgttgg gtatgatgat acagaagact cttcatgtta 1981 cattccgccg ctgactacga tcaaacagga ctttcggctg ctcggccaaa catctgttga 2041 ccgtttgcta caactgtccc agggtcaggc agtgaagggc aaccaactcc ttcctgttag 2101 tttggtcaaa cgtaaaacca ccctcgcccc taacacccaa actgcctcac cgcgcgcgct 2161 agccgatagt ctgatgcagc tggcacgtca ggtaagtcgt ttggaaagcg gtcagtacta 2221 gagtgtggaa ttgtgagcgc tcacaattcc acatactaga tggtcgacct gtgtgtcttc 2281 cgtaaactga tattgcagag ccgcgaaaaa aaaatgttat ttacggcgcc gaatccgaat 2341 acattgggta aactctattg ctggcgcaac gctctgcaat gtccctcggc gttcccgcat 2401 acggccagta acatcaggtg tgatggcatc gcggtcatct cccgaatttt ccgtcattac 2461 tgtcaggccc gaacgaaaag cagcaagctg tggattcagg atgaatgctc agttgacctt 2521 tgtgagcaga aaatttttca tccccattac ttctctctgg tatactggta tggttgttca 2581 acatgtcgtt tgcatcctcg tacgctgtcc cacggctata ttaaaaccaa tcttgtcttc 2641 actaccagga agatattacc taaggtactg gaagtaaaag atcggaccaa ctacatcaaa 2701 aggatcataa ttcttgacag tgaggaaaac ctgttaggct gtgaatctct gcataatttt 2761 atgagccgtt attcggataa taatctgcaa acgtttaaac ctcttcatta tgatcccgtt 2821 gatcaggtgg cagccatcct atgtagctca ggaaccacgg ggttgccaaa gggtgtgatg 2881 cagactcatc gtaacatatg cgtgcgtctc acacacgcga gtgaccctag agtcggcaca 2941 cagctgatcc cgggtgtgtc cgttctggca tatcttccat ttttccatgc atttggcttt 3001 tccataaatc tggggtattt catggttggc ctgcgggtcg ttatgttgcg caggtttaat 3061 caggaagttt tcctgaaagc aatccaggat tatgaggtcc gcagtgtgat aaacgttccg 3121 tcaaccattc tgtttctgtc caagtctcct ttggtcgata aatatgactt gctgtggcgc 3181 aactgcgtcg ctgctcttca tcaccagagg aaactgttac gacagaatga cacatgccag 3241 gaatttgtgg ttgacatggt tcaatcctta ctgcaattaa tctacatttt atttaccatg 3301 aacctcagcc aagatcattt agaaaagtct ttactgatct ggctgaggaa gattggcacg 3361 ctggtgaaac tgtgggatca gatcaaatta gaaaattacg cgagcggcgt gctctggcaa 3421 aaagttacaa ccatccacaa acctcctaaa cgtttgctta tgactacggt aggattcact 3481 cttgagacac tagataccat gatgcggatg aatatcagta tttggacagt gacccggaac 3541 ctcctgaaca ttaaggctat aagacacttg aataaacgct tttattataa cattcaggtt 3601 gaaatgcttt tatccctggt gtttctgatc aaactcgaga actatcaact ggggagctgg 3661 ctgaacaatc ccgaacataa tcttcaacag aaaaaattca ccatttttct gccgaacggg 3721 tcgttaattc aatccatctg tgtggaagaa tttgattctc tcatacaata tccgggcatg 3781 ctccaggtta agttccagga gaaaaacttc gaaagacggt ggaaaaaact gctgaacttc 3841 aaaagcctcg cctggattaa aaaagaatgg gatattttta ctaacaatat ttggattaac 3901 gcgcctatta aaaaaaagaa gaaattggtc gtagtggtgg cagcgctgca ctttccggtt 3961 gtttcagccg ctgtggttgt gttggtcgag ccgatggagc gagccagctt tacgagcacg 4021 ttctgttggc gaaaaggaac tcggaattgg tggtgccgca cgtgctgttc tattcggaag 4081 tgtaagtgga catgggggag tcaactgttt ttcctgtctc gcaaacgtac atctaccctc 4141 cagtactcgt gctctcagat cctaaactgc aataagtata atgcgcagaa ctcttacttt 4201 attaatttgc agtgtttctt atattctttt atgctcgttt taatcaccat attctttaat 4261 ttgatctttt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4321 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctc //