BBa_K233004 1 BBa_K233004 lac operator 2009-07-09T11:00:00Z 2015-05-08T01:11:35Z E.coli This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI false false _329_ 0 4258 9 It's complicated true had it synthesized false Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M1632 1 BBa_M1632 Our lactose mutant coupled to Hmd 2013-04-11T11:00:00Z 2015-05-08T01:13:57Z GenBank: AAB98710.1 + The gene sequence of BBa_M1631 The LacI sequence from BBa_M1631 together with the Hmd part, coded together to make a larger protein false false _768_ 0 16129 9 Not in stock false Put right next to each other, coded together. No stop codons inside false S. Gabrielle Gladstone annotation2214851 1 Beginning of LacI_mutant ORF range2214851 1 1075 1077 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_M1193 1 BBa_M1193 Gabi's part in GFP assay 2013-03-25T12:00:00Z 2015-05-08T01:13:54Z This is just a class exercise Something in the box false false _768_ 0 16129 9 Not in stock false Nope. false S. Gabrielle Gladstone component2216818 1 BBa_M1635 component2216812 1 BBa_J23119 component2216814 1 BBa_B0034 component2216817 1 BBa_K233004 component2216819 1 BBa_B0010 component2216816 1 BBa_M1632 annotation2216817 1 BBa_K233004 range2216817 1 2224 2253 annotation2216812 1 BBa_J23119 range2216812 1 1 35 annotation2216814 1 BBa_B0034 range2216814 1 44 55 annotation2216818 1 BBa_M1635 range2216818 1 2260 4275 annotation2216816 1 BBa_M1632 range2216816 1 62 2215 annotation2216819 1 BBa_B0010 range2216819 1 4284 4363 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M1635 1 BBa_M1635 Pyrearinus termitilluminans prothoracic luciferase 2013-04-23T11:00:00Z 2015-05-08T01:13:57Z AF116843 pH insensitive luciferase E.coli optimized cut sites taken out for standardized part can be used to report similar to GFP, but it glows and doesn't fluoresce false false _768_ 0 16143 9 Not in stock false pH insensitive luciferase E.coli optimized cut sites taken out for standardized part false Chauncey Tucker BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M1193_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaaaatcgccatattaggggcaggctgctaccggacacacgcagcagcaggtataacaaattttatgcgggcgtgtgaggtagctaaagaggtcggaaagcctgagattgcactaacgcattccagtatcacgtatggggccgagctgctccatctcgtcccagatgtaaaggaggtaattgttagcgatccgtgttttgcagaagagcccggcctggtcgtcattgatgagtttgacccaaaagaggtgatggaggcccatcttagcggtaatccggaaagtattatgcccaaaatccgggaagtcgtgaaggctaaagcgaaagagttaccaaaaccccccaaggcatgcatccatctggtacatccggaagacgtggggctgaaagttacgagcgatgatcgcgaggccgttgagggagccgacatcgtgatcacatggttaccgaaggggaacaagcagcccgatataattaaaaaatttgccgatgccataccggaaggagcgatcgttacccacgcatgtacgatcccaacgaccaaattcgcaaagattttcaaagatctaggacgtgaagatctgaacattacatcataccacccgggttgcgtgcctgagatgaaggggcaagtatacattgcggaaggatacgcttctgaagaagctgtgaacaaactgtacgaaattggtaaaatcgcgcgggggaaagcgttcaaaatgcccgcaaacctgatcggtccggtttgcgacatgtgttcagctgtcaccgccacggtatatgcgggacttttagcatacagagatgcggttaccaaaattttaggagcaccagctgacttcgcgcaaatgatggccgatgaggccctgacccaaatccataatctgatgaaagaaaaaggcattgcaaatatggaggaagctttggacccggccgctttgctgggtaccgccgacagtatgtgctttggcccgctggcagagatcttacccactgctctgaaagtgctggagaaacacaaagttgtggaagaggagggtaaaaccaagtgcgagataatgtcgcagaaagaaatgaaacctgtgactctgtatgatgttgcggagtatgcaggtgtctcgtaccagaccgtgagccgcgttgttaaccaagctagtcatgtcagcgcgaaaacgcgtgagaaagtggaagctgcaatggcggaattgaactacattccaaatcgtgtcgcacagcaactagcgggcaaacaatctcttctgataggcgtagctacgagttctctggcattgcaccatcactcccaatgtgttgcagcgataaagtcacgtgcagatcaattgggtgcgtcggtggtcgtgtctatggtggaaagatcaggtgtggaagcatgcaaagcagcggttcataatctgctggcacaacgtgtatcaggactactgattaactatccccttgacgatcaagatgcgacggccgttgaagccgcatgcgctaatgtccctgcccttttcttagacgttagtgatcaaactccgatcaattcaatcatttttagccatgaggatggcactcggcttggcgtagagcacttagtagcgttgggacatcaacagattgctcttttggcgggcccattaagcagtgtgtcagcgcgtttgcgactagccggctggcacaaatatttgacccgcaaccaaatccagccgattgctgagcgggagggcgattggtcggcaatgtctggctttcagcaaacgatgcaaatgctgaatgaaggaatcgtcccaactgcaatgctggtggctaacgatcagatggccttaggcgcgatgcgtgcaatcaccgagtctggactgcgggtgggagcagatatatcggtcgttgggtatgatgatacagaagactcttcatgttacattccgccgctgactacgatcaaacaggactttcggctgctcggccaaacatctgttgaccgtttgctacaactgtcccagggtcaggcagtgaagggcaaccaactccttcctgttagtttggtcaaacgtaaaaccaccctcgcccctaacacccaaactgcctcaccgcgcgcgctagccgatagtctgatgcagctggcacgtcaggtaagtcgtttggaaagcggtcagtactagagtgtggaattgtgagcgctcacaattccacatactagatggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K233004_sequence 1 tgtggaattgtgagcgctcacaattccaca BBa_B0034_sequence 1 aaagaggagaaa BBa_M1632_sequence 1 atgaaaatcgccatattaggggcaggctgctaccggacacacgcagcagcaggtataacaaattttatgcgggcgtgtgaggtagctaaagaggtcggaaagcctgagattgcactaacgcattccagtatcacgtatggggccgagctgctccatctcgtcccagatgtaaaggaggtaattgttagcgatccgtgttttgcagaagagcccggcctggtcgtcattgatgagtttgacccaaaagaggtgatggaggcccatcttagcggtaatccggaaagtattatgcccaaaatccgggaagtcgtgaaggctaaagcgaaagagttaccaaaaccccccaaggcatgcatccatctggtacatccggaagacgtggggctgaaagttacgagcgatgatcgcgaggccgttgagggagccgacatcgtgatcacatggttaccgaaggggaacaagcagcccgatataattaaaaaatttgccgatgccataccggaaggagcgatcgttacccacgcatgtacgatcccaacgaccaaattcgcaaagattttcaaagatctaggacgtgaagatctgaacattacatcataccacccgggttgcgtgcctgagatgaaggggcaagtatacattgcggaaggatacgcttctgaagaagctgtgaacaaactgtacgaaattggtaaaatcgcgcgggggaaagcgttcaaaatgcccgcaaacctgatcggtccggtttgcgacatgtgttcagctgtcaccgccacggtatatgcgggacttttagcatacagagatgcggttaccaaaattttaggagcaccagctgacttcgcgcaaatgatggccgatgaggccctgacccaaatccataatctgatgaaagaaaaaggcattgcaaatatggaggaagctttggacccggccgctttgctgggtaccgccgacagtatgtgctttggcccgctggcagagatcttacccactgctctgaaagtgctggagaaacacaaagttgtggaagaggagggtaaaaccaagtgcgagataatgtcgcagaaagaaatgaaacctgtgactctgtatgatgttgcggagtatgcaggtgtctcgtaccagaccgtgagccgcgttgttaaccaagctagtcatgtcagcgcgaaaacgcgtgagaaagtggaagctgcaatggcggaattgaactacattccaaatcgtgtcgcacagcaactagcgggcaaacaatctcttctgataggcgtagctacgagttctctggcattgcaccatcactcccaatgtgttgcagcgataaagtcacgtgcagatcaattgggtgcgtcggtggtcgtgtctatggtggaaagatcaggtgtggaagcatgcaaagcagcggttcataatctgctggcacaacgtgtatcaggactactgattaactatccccttgacgatcaagatgcgacggccgttgaagccgcatgcgctaatgtccctgcccttttcttagacgttagtgatcaaactccgatcaattcaatcatttttagccatgaggatggcactcggcttggcgtagagcacttagtagcgttgggacatcaacagattgctcttttggcgggcccattaagcagtgtgtcagcgcgtttgcgactagccggctggcacaaatatttgacccgcaaccaaatccagccgattgctgagcgggagggcgattggtcggcaatgtctggctttcagcaaacgatgcaaatgctgaatgaaggaatcgtcccaactgcaatgctggtggctaacgatcagatggccttaggcgcgatgcgtgcaatcaccgagtctggactgcgggtgggagcagatatatcggtcgttgggtatgatgatacagaagactcttcatgttacattccgccgctgactacgatcaaacaggactttcggctgctcggccaaacatctgttgaccgtttgctacaactgtcccagggtcaggcagtgaagggcaaccaactccttcctgttagtttggtcaaacgtaaaaccaccctcgcccctaacacccaaactgcctcaccgcgcgcgctagccgatagtctgatgcagctggcacgtcaggtaagtcgtttggaaagcggtcag BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_M1635_sequence 1 atggtcgacctgtgtgtcttccgtaaactgatattgcagagccgcgaaaaaaaaatgttatttacggcgccgaatccgaatacattgggtaaactctattgctggcgcaacgctctgcaatgtccctcggcgttcccgcatacggccagtaacatcaggtgtgatggcatcgcggtcatctcccgaattttccgtcattactgtcaggcccgaacgaaaagcagcaagctgtggattcaggatgaatgctcagttgacctttgtgagcagaaaatttttcatccccattacttctctctggtatactggtatggttgttcaacatgtcgtttgcatcctcgtacgctgtcccacggctatattaaaaccaatcttgtcttcactaccaggaagatattacctaaggtactggaagtaaaagatcggaccaactacatcaaaaggatcataattcttgacagtgaggaaaacctgttaggctgtgaatctctgcataattttatgagccgttattcggataataatctgcaaacgtttaaacctcttcattatgatcccgttgatcaggtggcagccatcctatgtagctcaggaaccacggggttgccaaagggtgtgatgcagactcatcgtaacatatgcgtgcgtctcacacacgcgagtgaccctagagtcggcacacagctgatcccgggtgtgtccgttctggcatatcttccatttttccatgcatttggcttttccataaatctggggtatttcatggttggcctgcgggtcgttatgttgcgcaggtttaatcaggaagttttcctgaaagcaatccaggattatgaggtccgcagtgtgataaacgttccgtcaaccattctgtttctgtccaagtctcctttggtcgataaatatgacttgctgtggcgcaactgcgtcgctgctcttcatcaccagaggaaactgttacgacagaatgacacatgccaggaatttgtggttgacatggttcaatccttactgcaattaatctacattttatttaccatgaacctcagccaagatcatttagaaaagtctttactgatctggctgaggaagattggcacgctggtgaaactgtgggatcagatcaaattagaaaattacgcgagcggcgtgctctggcaaaaagttacaaccatccacaaacctcctaaacgtttgcttatgactacggtaggattcactcttgagacactagataccatgatgcggatgaatatcagtatttggacagtgacccggaacctcctgaacattaaggctataagacacttgaataaacgcttttattataacattcaggttgaaatgcttttatccctggtgtttctgatcaaactcgagaactatcaactggggagctggctgaacaatcccgaacataatcttcaacagaaaaaattcaccatttttctgccgaacgggtcgttaattcaatccatctgtgtggaagaatttgattctctcatacaatatccgggcatgctccaggttaagttccaggagaaaaacttcgaaagacggtggaaaaaactgctgaacttcaaaagcctcgcctggattaaaaaagaatgggatatttttactaacaatatttggattaacgcgcctattaaaaaaaagaagaaattggtcgtagtggtggcagcgctgcactttccggttgtttcagccgctgtggttgtgttggtcgagccgatggagcgagccagctttacgagcacgttctgttggcgaaaaggaactcggaattggtggtgccgcacgtgctgttctattcggaagtgtaagtggacatgggggagtcaactgtttttcctgtctcgcaaacgtacatctaccctccagtactcgtgctctcagatcctaaactgcaataagtataatgcgcagaactcttactttattaatttgcagtgtttcttatattcttttatgctcgttttaatcaccatattctttaatttgatcttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z