LOCUS BBa_M1205 3794 bp DNA linear 22-Nov-2024 DEFINITION Complete Part for ROSE Regulated Kill Gene ACCESSION BBa_M1205 VERSION BBa_M1205.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI misc_feature 4..31 /label=dif site (forward) misc_feature 1..31 /label=BBa_I742101 promoter 40..231 /label=BBa_J22106 RBS 240..251 /label=BBa_J61100 RBS 349..354 /label=SD misc_feature 333..338 /label=Biobrick-Scar-adaptation stem_loop 260..274 /label=Predicted Stem Loop stem_loop 275..295 /label=Predicted Stem Loop stem_loop 298..330 /label=Predicted Stem Loop stem_loop 331..355 /label=Predicted Stem Loop extending to startcodon misc_feature 260..355 /label=BBa_K115001 CDS 365..981 /label=tetR misc_feature 982..1015 /label=SsrA misc_feature 1022..1046 /label=Help:Barcodes CDS 362..1046 /label=BBa_C0040 stem_loop 1066..1109 /label=stem_loop misc_feature 1055..1134 /label=BBa_B0010 terminator 1055..1134 /label=BBa_B0010 misc_feature 1143..1161 /label=TetR 1 misc_feature 1143..1196 /label=BBa_R0040 promoter 1162..1167 /label=-35 misc_feature 1168..1186 /label=TetR 2 promoter 1185..1190 /label=-10 promoter 1143..1196 /label=BBa_R0040 RBS 1205..1216 /label=BBa_J61102 CDS 1223..1942 /label=GFP protein CDS 1223..1942 /label=BBa_E0040 CDS 1967..2623 /label=T4 holin CDS 1951..2626 /label=BBa_K112805 CDS 2651..3145 /label=T4 Endolysin CDS 2635..3148 /label=BBa_K112806 stem_loop 3168..3211 /label=stem_loop misc_feature 3157..3236 /label=BBa_B0010 terminator 3157..3236 /label=BBa_B0010 stem_loop 3252..3271 /label=T7 TE misc_feature 3278..3278 /label=stop polyA_site 3272..3285 /label=polya misc_feature 3245..3285 /label=BBa_B0012 terminator 3245..3285 /label=BBa_B0012 terminator 3157..3285 /label=BBa_B0015 promoter 3294..3328 /label=BBa_J23116 RBS 3337..3348 /label=BBa_J61101 CDS 3371..3664 /label=T4 antiholin CDS 3357..3667 /label=BBa_K112807 stem_loop 3687..3730 /label=stem_loop misc_feature 3676..3755 /label=BBa_B0010 terminator 3676..3755 /label=BBa_B0010 misc_feature 3764..3766 /label=SacI misc_feature 3767..3794 /label=dif site (reverse) misc_feature 3764..3794 /label=BBa_I742102 ORIGIN 1 ctcggtgcgc ataatgtata ttatgttaaa ttactagaga acaatttcta caaaacactt 61 gatactgtat gagcatacag tataattgct tcaacagaac atattgacta tccggtatta 121 cccggcatga caggagtaaa aatggctatc gacgaaaaca aacagaaagc gttggcggca 181 gcactgggcc agattgagaa acaatttggt aaaggctcca tcatgtaata atactagaga 241 aagaggggac atactagagg ccgcgacaag cggtccgggc gccctagggg cccggcggag 301 acgggcgccg gaggtgtccg acgcctgctc gtcaagttct tgctccttgg aggattacta 361 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 421 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 481 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 541 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 601 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 661 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 721 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 781 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 841 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 901 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 961 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 1021 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 1081 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 1141 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact 1201 agagaaagat ccgatgtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc 1261 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 1321 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 1381 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 1441 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 1501 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 1561 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 1621 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 1681 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 1741 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 1801 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 1861 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 1921 ggatgaacta tacaaataat aatactagag cttaaaagga gggtctatgg cagcacctag 1981 aatatcattt tcgccctctg atattctatt tggtgttctc gatcgcttgt tcaaagataa 2041 cgctaccggg aaggttcttg cttcccgggt agctgtcgta attcttttgt ttataatggc 2101 gattgtttgg tataggggag atagtttctt tgagtactat aagcaatcaa agtatgaaac 2161 atacagtgaa attattgaaa aggaaagaac tgcacgcttt gaatctgtcg ccctggaaca 2221 actccagata gttcatatat catctgaggc agactttagt gcggtgtatt ctttccgccc 2281 taaaaactta aactattttg ttgatattat agcatacgaa ggaaaattac cttcaacaat 2341 aagtgaaaaa tcacttggag gatatcctgt tgataaaact atggatgaat atacagttca 2401 tttaaatgga cgtcattatt attccaactc aaaatttgct tttttaccaa ctaaaaagcc 2461 tactcccgaa ataaactaca tgtacagttg tccatatttt aatttggata atatctatgc 2521 tggaacgata accatgtact ggtatagaaa tgatcatata agtaatgacc gccttgaatc 2581 aatatgtgct caggcggcca gaatattagg aagggctaaa taattatact agagatactt 2641 aggaggtatt atgaatatat ttgaaatgtt acgtatagat gaaggtctta gacttaaaat 2701 ctataaagac acagaaggct attacactat tggcatcggt catttgctta caaaaagtcc 2761 atcacttaat gctgctaaat ctgaattaga taaagctatt gggcgtaatt gcaatggtgt 2821 aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg ctgttcgcgg 2881 aatcctgaga aatgctaaat taaaaccggt ttatgattct cttgatgcgg ttcgtcgctg 2941 tgcattgatt aatatggttt tccaaatggg agaaaccggt gtggcaggat ttactaactc 3001 tttacgtatg cttcaacaaa aacgctggga tgaagcagca gttaacttag ctaaaagtag 3061 atggtataat caaacaccta atcgcgcaaa acgagtcatt acaacgttta gaactggcac 3121 ttgggacgcg tataaaaatc tataaagcta ctagagccag gcatcaaata aaacgaaagg 3181 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3241 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag 3301 ctagctcagt cctagggact atgctagcta ctagagaaag acaggaccta ctagaggata 3361 ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt 3421 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg 3481 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta 3541 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga 3601 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga 3661 atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3721 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagctcattt aacataatat 3781 acattatgcg cacc //