##gff-version 3
##sequence-region BBa_M1205 1 3794
BBa_M1205	.	promoter	3294	3328	.	+	0	ID=BBa_J23116;Name=BBa_J23116
BBa_M1205	.	mature_transcript_region	260	355	.	+	0	ID=BBa_K115001;Name=BBa_K115001
BBa_M1205	.	sequence_alteration	333	338	.	+	0	ID=annotation1966898;Name=Biobrick-Scar-adaptation;Parent=BBa_K115001
BBa_M1205	.	stem_loop	275	295	.	+	0	ID=annotation1966927;Name=Predicted Stem Loop;Parent=BBa_K115001
BBa_M1205	.	stem_loop	260	274	.	+	0	ID=annotation1966926;Name=Predicted Stem Loop;Parent=BBa_K115001
BBa_M1205	.	stem_loop	298	330	.	+	0	ID=annotation1966928;Name=Predicted Stem Loop;Parent=BBa_K115001
BBa_M1205	.	stem_loop	331	355	.	+	0	ID=annotation1966929;Name=Predicted Stem Loop extending to startcodon;Parent=BBa_K115001
BBa_M1205	.	ribosome_entry_site	349	354	.	+	0	ID=annotation1966896;Name=SD;Parent=BBa_K115001
BBa_M1205	.	promoter	1143	1196	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_M1205	.	non_covalent_binding_site	1143	1161	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_M1205	.	promoter	1185	1190	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_M1205	.	non_covalent_binding_site	1168	1186	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_M1205	.	promoter	1162	1167	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_M1205	.	engineered_region	1143	1196	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_M1205	.	ribosome_entry_site	3337	3348	.	+	0	ID=BBa_J61101;Name=BBa_J61101
BBa_M1205	.	terminator	3676	3755	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1205	.	engineered_region	3676	3755	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1205	.	stem_loop	3687	3730	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1205	.	CDS	1951	2626	.	+	0	ID=BBa_K112805;Name=BBa_K112805
BBa_M1205	.	CDS	1967	2623	.	+	0	ID=annotation1999005;Name=T4 holin;Parent=BBa_K112805
BBa_M1205	.	sequence_feature	3764	3794	.	+	0	ID=BBa_I742102;Name=BBa_I742102
BBa_M1205	.	sequence_feature	3767	3794	.	+	0	ID=annotation1955265;Name=dif site (reverse);Parent=BBa_I742102
BBa_M1205	.	sequence_feature	3764	3766	.	+	0	ID=annotation1940993;Name=SacI;Parent=BBa_I742102
BBa_M1205	.	ribosome_entry_site	240	251	.	+	0	ID=BBa_J61100;Name=BBa_J61100
BBa_M1205	.	CDS	1223	1942	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_M1205	.	CDS	1223	1942	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_M1205	.	sequence_feature	1	31	.	+	0	ID=BBa_I742101;Name=BBa_I742101
BBa_M1205	.	sequence_feature	4	31	.	+	0	ID=annotation1955264;Name=dif site (forward);Parent=BBa_I742101
BBa_M1205	.	sequence_feature	1	3	.	+	0	ID=annotation1940992;Name=SacI;Parent=BBa_I742101
BBa_M1205	.	terminator	1055	1134	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1205	.	engineered_region	1055	1134	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1205	.	stem_loop	1066	1109	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1205	.	CDS	3357	3667	.	+	0	ID=BBa_K112807;Name=BBa_K112807
BBa_M1205	.	CDS	3371	3664	.	+	0	ID=annotation1999019;Name=T4 antiholin;Parent=BBa_K112807
BBa_M1205	.	CDS	362	1046	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_M1205	.	CDS	365	981	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_M1205	.	tag	982	1015	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_M1205	.	engineered_tag	1022	1046	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_M1205	.	promoter	40	231	.	+	0	ID=BBa_J22106;Name=BBa_J22106
BBa_M1205	.	terminator	3157	3285	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1205	.	terminator	3157	3236	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1205	.	engineered_region	3157	3236	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1205	.	stem_loop	3168	3211	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1205	.	terminator	3245	3285	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1205	.	engineered_region	3245	3285	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1205	.	stop_codon	3278	3278	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1205	.	polyA_site	3272	3285	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1205	.	stem_loop	3252	3271	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1205	.	CDS	2635	3148	.	+	0	ID=BBa_K112806;Name=BBa_K112806
BBa_M1205	.	CDS	2651	3145	.	+	0	ID=annotation1999018;Name=T4 Endolysin;Parent=BBa_K112806
BBa_M1205	.	ribosome_entry_site	1205	1216	.	+	0	ID=BBa_J61102;Name=BBa_J61102
>BBa_M1205
ctcggtgcgcataatgtatattatgttaaattactagagaacaatttctacaaaacacttgatactgtatgagcataca
gtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaa
caaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataatactag
agaaagaggggacatactagaggccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtg
tccgacgcctgctcgtcaagttcttgctccttggaggattactagatgtccagattagataaaagtaaagtgattaaca
gcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagca
gcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccat
actcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttac
taagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatt
agcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggt
tgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattat
tacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgg
attagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactga
tagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtccctatcagtgatagagattgacatccctatcagtgatagag
atactgagcactactagagaaagatccgatgtactagatgcgtaaaggagaagaacttttcactggagttgtcccaatt
cttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaa
aacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttg
ttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataa
ctcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaa
gatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacc
attacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaac
agctgctgggattacacatggcatggatgaactatacaaataataatactagagcttaaaaggagggtctatggcagca
cctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaagg
ttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttga
gtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgcc
ctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaa
actattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgt
tgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttacca
actaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacga
taaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatatt
aggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggt
cttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcac
ttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaa
actctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctctt
gatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctt
tacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaa
tcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggga
ctatgctagctactagagaaagacaggacctactagaggataggaggcctttatggccttaaaagcaacagcacttttt
gccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaat
ctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaac
gacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctaga
attatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagctcatttaacataatatacattatgcgca
cc