##gff-version 3
##sequence-region BBa_M12069 1 2646
BBa_M12069	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M12069	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M12069	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M12069	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M12069	.	terminator	24	103	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M12069	.	stem_loop	35	78	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M12069	.	engineered_region	24	103	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M12069	.	promoter	112	2067	.	+	0	ID=BBa_K112707;Name=BBa_K112707
BBa_M12069	.	CDS	2074	2646	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_M12069	.	sequence_alteration	2143	2148	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_M12069	.	CDS	2074	2646	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_M12069	.	sequence_alteration	2145	2145	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_M12069	.	primer_binding_site	2074	2076	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_M12069	.	primer_binding_site	2624	2643	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
>BBa_M12069
attaaagaggagaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagagatcctgtgatgtttggcgattgatcagatcgcacaatccgggctg
agttccctttcagtgatctactattttgcgaagctatttagtgcacactaatcgatttttcagacaacctttctcgcct
ggcgtgagttttcgttcgactgaacctataaaaaggctctgcattctgtcctgtagtgcgccaggcctaataacgcctc
ccgtttaattccggcccggtcgtacatcggttctgttcatttatgggatcgcgactttttcgttgtaatgcttcaagag
cgattttttgttctgcggtcaactcaacattcatagccatcagcatgatcctgattatttttgaaaccaagcatcttca
ttgatcatgggtatacatcgttgttatccagctatacatcataacaggtcaattaaatccactcagaaataaagtcagg
gtatgcataaaatctatcgcccataatcctatccagtaaccccatgattagtaagaactaatcagtctgtgcaaaaaca
ggtcaccgccatcctgtttttgcacatcaaaacattttttcaggattattctgaaaaaaaaaaggagatattatggatt
tcttgattaataaaaaattaaaaattttcataacactgatggaaacaggttccttcagtatcgcaacatcagtactgta
tatcacccgaaccccgctgagcagggttatttcagacctggaaagagagctgaaacaaagactctttatacggaagaat
ggcactcttatcccaaccgaatttgcacaaactatttatcgaaaagtaaaatcccattatattttcttacatgcactgg
agcaggaaatcggacctacgggtaaaacgaaacaactagaaataatatttgacgaaatttatccgggaagtttaaaaaa
tctgatcatttcagcactgaccatttccggccaaaaaacaaatataatggggagagccgttaacagccaaataatagaa
gaactgtgtcagacaaacaactgcattgttatttctgccagaaattattttcatcgggaatcgcttgtctgccggacat
cagtggagggtggggtcatgttatttattcctaaaaaattctttctctgcggcaaacctgatatcaacaggctggccgg
aacacctgtactttttcatgagggggctaaaaattttaatctggacaccatataccatttttttgaacagacactaggt
attaccaaccctgcattcagttttgataacgtcgatttgttcagttcactgtaccggttacaacaagggctggcgatgt
tactcatccccgtcagagtctgtcgggctctgggattatcaacagatcacgcactgcacatcaaaggcgtagcgctctg
tacctccttgtattacccgaccaagaaacgggagacaccagattatcgtaaagctataaaactgatacagcaggaactg
aaacagtccaccttctgaccttatgcagcgtaagggccgcaacacctgtattcacggcatttgccagattcagattgtc
agcaatccccatcctccatagcggtagttcaccgcggagcatggagtaaaccggctggtcgccgtcaatctgacacaga
atcagtttgatgctctggtggattacctaaaacatgggcattaacgcgctggctcacgccactttactgaagaaactga
ataacggtgactatgacggcgcagcgaatgaattaattcctgaaatgggaccacgccagcggtcaggttgttcccggcc
tgacccgacgccggagcgctgaacgttgcttattcctgagttaatttgttgtgccatctttgcacaccgggaaccgcga
ttccgcacagcagaaaaatagcacataaataaactcaatataagccactcattttctggcaatacaaaataattcccct
gcaaacattatcgtactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgt
aatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctga
aacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaa
tctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagc
gcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttg
ccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagat
tagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacaga
tactttccggcaagcagtataaaaaaacgaatgaattaa