LOCUS       BBa_M1207               1007 bp    DNA     linear       15-Nov-2024
DEFINITION  Temperature Sensitive Hairpin Loop + Tet Repressor
ACCESSION   BBa_M1207
VERSION     BBa_M1207.1  
FEATURES             Location/Qualifiers
     promoter        1..192
                     /label=BBa_J22106
     RBS             201..212
                     /label=BBa_J61100
     RBS             310..315
                     /label=SD
     misc_feature    294..299
                     /label=Biobrick-Scar-adaptation
     stem_loop       221..235
                     /label=Predicted Stem Loop
     stem_loop       236..256
                     /label=Predicted Stem Loop
     stem_loop       259..291
                     /label=Predicted Stem Loop
     stem_loop       292..316
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    221..316
                     /label=BBa_K115001
     CDS             326..942
                     /label=tetR
     misc_feature    943..976
                     /label=SsrA
     misc_feature    983..1007
                     /label=Help:Barcodes
     CDS             323..1007
                     /label=BBa_C0040
ORIGIN
        1 aacaatttct acaaaacact tgatactgta tgagcataca gtataattgc ttcaacagaa
       61 catattgact atccggtatt acccggcatg acaggagtaa aaatggctat cgacgaaaac
      121 aaacagaaag cgttggcggc agcactgggc cagattgaga aacaatttgg taaaggctcc
      181 atcatgtaat aatactagag aaagagggga catactagag gccgcgacaa gcggtccggg
      241 cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc gacgcctgct cgtcaagttc
      301 ttgctccttg gaggattact agatgtccag attagataaa agtaaagtga ttaacagcgc
      361 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
      421 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
      481 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
      541 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
      601 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
      661 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
      721 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
      781 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
      841 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
      901 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa
      961 ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcac
//