##gff-version 3
##sequence-region BBa_M12070 1 2646
BBa_M12070	.	terminator	1965	2044	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M12070	.	stem_loop	1976	2019	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M12070	.	engineered_region	1965	2044	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M12070	.	promoter	1	1956	.	+	0	ID=BBa_K112707;Name=BBa_K112707
BBa_M12070	.	ribosome_entry_site	2632	2646	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M12070	.	engineered_region	2632	2646	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M12070	.	ribosome_entry_site	2632	2646	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M12070	.	ribosome_entry_site	2639	2643	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M12070	.	CDS	2051	2623	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_M12070	.	CDS	2051	2623	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_M12070	.	sequence_alteration	2122	2122	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_M12070	.	sequence_alteration	2120	2125	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_M12070	.	primer_binding_site	2051	2053	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_M12070	.	primer_binding_site	2601	2620	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
>BBa_M12070
agatcctgtgatgtttggcgattgatcagatcgcacaatccgggctgagttccctttcagtgatctactattttgcgaa
gctatttagtgcacactaatcgatttttcagacaacctttctcgcctggcgtgagttttcgttcgactgaacctataaa
aaggctctgcattctgtcctgtagtgcgccaggcctaataacgcctcccgtttaattccggcccggtcgtacatcggtt
ctgttcatttatgggatcgcgactttttcgttgtaatgcttcaagagcgattttttgttctgcggtcaactcaacattc
atagccatcagcatgatcctgattatttttgaaaccaagcatcttcattgatcatgggtatacatcgttgttatccagc
tatacatcataacaggtcaattaaatccactcagaaataaagtcagggtatgcataaaatctatcgcccataatcctat
ccagtaaccccatgattagtaagaactaatcagtctgtgcaaaaacaggtcaccgccatcctgtttttgcacatcaaaa
cattttttcaggattattctgaaaaaaaaaaggagatattatggatttcttgattaataaaaaattaaaaattttcata
acactgatggaaacaggttccttcagtatcgcaacatcagtactgtatatcacccgaaccccgctgagcagggttattt
cagacctggaaagagagctgaaacaaagactctttatacggaagaatggcactcttatcccaaccgaatttgcacaaac
tatttatcgaaaagtaaaatcccattatattttcttacatgcactggagcaggaaatcggacctacgggtaaaacgaaa
caactagaaataatatttgacgaaatttatccgggaagtttaaaaaatctgatcatttcagcactgaccatttccggcc
aaaaaacaaatataatggggagagccgttaacagccaaataatagaagaactgtgtcagacaaacaactgcattgttat
ttctgccagaaattattttcatcgggaatcgcttgtctgccggacatcagtggagggtggggtcatgttatttattcct
aaaaaattctttctctgcggcaaacctgatatcaacaggctggccggaacacctgtactttttcatgagggggctaaaa
attttaatctggacaccatataccatttttttgaacagacactaggtattaccaaccctgcattcagttttgataacgt
cgatttgttcagttcactgtaccggttacaacaagggctggcgatgttactcatccccgtcagagtctgtcgggctctg
ggattatcaacagatcacgcactgcacatcaaaggcgtagcgctctgtacctccttgtattacccgaccaagaaacggg
agacaccagattatcgtaaagctataaaactgatacagcaggaactgaaacagtccaccttctgaccttatgcagcgta
agggccgcaacacctgtattcacggcatttgccagattcagattgtcagcaatccccatcctccatagcggtagttcac
cgcggagcatggagtaaaccggctggtcgccgtcaatctgacacagaatcagtttgatgctctggtggattacctaaaa
catgggcattaacgcgctggctcacgccactttactgaagaaactgaataacggtgactatgacggcgcagcgaatgaa
ttaattcctgaaatgggaccacgccagcggtcaggttgttcccggcctgacccgacgccggagcgctgaacgttgctta
ttcctgagttaatttgttgtgccatctttgcacaccgggaaccgcgattccgcacagcagaaaaatagcacataaataa
actcaatataagccactcattttctggcaatacaaaataattcccctgcaaacattatcgtactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagatgg
ctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattg
tgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaat
aaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaat
tacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttca
tgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcg
caaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggcc
atcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaa
aaaacgaatgaattaatactagagattaaagaggagaaa