BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J31000 1 Hin DNA-invertase Hin from Salmonella typhimurium 2006-06-04T11:00:00Z 2015-08-31T04:08:45Z Salmonella typhimurium Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds. false false _61_ 0 918 61 It's complicated false none really false Erin Zwack, Sabriya Rosemond annotation1880459 1 Former SpeI site range1880459 1 70 75 annotation1910568 1 Fwd primer J31001 range1910568 1 1 3 annotation1910569 1 Rev primer J31001 range1910569 1 551 570 annotation1884987 1 Hin sequence range1884987 1 1 573 annotation1880460 1 Mutation to delete the SpeI site range1880460 1 72 72 BBa_M12071 1 BBa_M12071 hin invertase under constitutive promoter pspv from Salmonella 2010-05-11T11:00:00Z 2015-05-08T01:13:55Z This device comes from multiple BioBrick parts. This part codes for the protein hin, which acts a flippase. The promoter under this device is constitutive. false false _548_ 0 6718 9 Not in stock false We needed the flippase to act with a constitutive promoter in order to serve the function for the coin flipper device in our system. false Logan Trimble component2069190 1 BBa_K112707 component2069200 1 BBa_B0010 component2069199 1 BBa_J31000 component2069192 1 BBa_B0030 annotation2069190 1 BBa_K112707 range2069190 1 1 1956 annotation2069200 1 BBa_B0010 range2069200 1 2567 2646 annotation2069199 1 BBa_J31000 range2069199 1 1986 2558 annotation2069192 1 BBa_B0030 range2069192 1 1965 1979 BBa_K112707 1 BBa_K112707 Pspv from <I>Salmonella</I> 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of <Pspv> basic part K112707 PCR of dv017/dv016 on pBca1037 (499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+499, L) Product is pBca1256-K112707 ----------------------------------------------- dv017 Forward biobricking of <Pspv> cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG dv016 Reverse biobricking of <Pspv> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG </pre> The spv promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0030_sequence 1 attaaagaggagaaa BBa_M12071_sequence 1 agatcctgtgatgtttggcgattgatcagatcgcacaatccgggctgagttccctttcagtgatctactattttgcgaagctatttagtgcacactaatcgatttttcagacaacctttctcgcctggcgtgagttttcgttcgactgaacctataaaaaggctctgcattctgtcctgtagtgcgccaggcctaataacgcctcccgtttaattccggcccggtcgtacatcggttctgttcatttatgggatcgcgactttttcgttgtaatgcttcaagagcgattttttgttctgcggtcaactcaacattcatagccatcagcatgatcctgattatttttgaaaccaagcatcttcattgatcatgggtatacatcgttgttatccagctatacatcataacaggtcaattaaatccactcagaaataaagtcagggtatgcataaaatctatcgcccataatcctatccagtaaccccatgattagtaagaactaatcagtctgtgcaaaaacaggtcaccgccatcctgtttttgcacatcaaaacattttttcaggattattctgaaaaaaaaaaggagatattatggatttcttgattaataaaaaattaaaaattttcataacactgatggaaacaggttccttcagtatcgcaacatcagtactgtatatcacccgaaccccgctgagcagggttatttcagacctggaaagagagctgaaacaaagactctttatacggaagaatggcactcttatcccaaccgaatttgcacaaactatttatcgaaaagtaaaatcccattatattttcttacatgcactggagcaggaaatcggacctacgggtaaaacgaaacaactagaaataatatttgacgaaatttatccgggaagtttaaaaaatctgatcatttcagcactgaccatttccggccaaaaaacaaatataatggggagagccgttaacagccaaataatagaagaactgtgtcagacaaacaactgcattgttatttctgccagaaattattttcatcgggaatcgcttgtctgccggacatcagtggagggtggggtcatgttatttattcctaaaaaattctttctctgcggcaaacctgatatcaacaggctggccggaacacctgtactttttcatgagggggctaaaaattttaatctggacaccatataccatttttttgaacagacactaggtattaccaaccctgcattcagttttgataacgtcgatttgttcagttcactgtaccggttacaacaagggctggcgatgttactcatccccgtcagagtctgtcgggctctgggattatcaacagatcacgcactgcacatcaaaggcgtagcgctctgtacctccttgtattacccgaccaagaaacgggagacaccagattatcgtaaagctataaaactgatacagcaggaactgaaacagtccaccttctgaccttatgcagcgtaagggccgcaacacctgtattcacggcatttgccagattcagattgtcagcaatccccatcctccatagcggtagttcaccgcggagcatggagtaaaccggctggtcgccgtcaatctgacacagaatcagtttgatgctctggtggattacctaaaacatgggcattaacgcgctggctcacgccactttactgaagaaactgaataacggtgactatgacggcgcagcgaatgaattaattcctgaaatgggaccacgccagcggtcaggttgttcccggcctgacccgacgccggagcgctgaacgttgcttattcctgagttaatttgttgtgccatctttgcacaccgggaaccgcgattccgcacagcagaaaaatagcacataaataaactcaatataagccactcattttctggcaatacaaaataattcccctgcaaacattatcgtactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J31000_sequence 1 atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaa BBa_K112707_sequence 1 agatcctgtgatgtttggcgattgatcagatcgcacaatccgggctgagttccctttcagtgatctactattttgcgaagctatttagtgcacactaatcgatttttcagacaacctttctcgcctggcgtgagttttcgttcgactgaacctataaaaaggctctgcattctgtcctgtagtgcgccaggcctaataacgcctcccgtttaattccggcccggtcgtacatcggttctgttcatttatgggatcgcgactttttcgttgtaatgcttcaagagcgattttttgttctgcggtcaactcaacattcatagccatcagcatgatcctgattatttttgaaaccaagcatcttcattgatcatgggtatacatcgttgttatccagctatacatcataacaggtcaattaaatccactcagaaataaagtcagggtatgcataaaatctatcgcccataatcctatccagtaaccccatgattagtaagaactaatcagtctgtgcaaaaacaggtcaccgccatcctgtttttgcacatcaaaacattttttcaggattattctgaaaaaaaaaaggagatattatggatttcttgattaataaaaaattaaaaattttcataacactgatggaaacaggttccttcagtatcgcaacatcagtactgtatatcacccgaaccccgctgagcagggttatttcagacctggaaagagagctgaaacaaagactctttatacggaagaatggcactcttatcccaaccgaatttgcacaaactatttatcgaaaagtaaaatcccattatattttcttacatgcactggagcaggaaatcggacctacgggtaaaacgaaacaactagaaataatatttgacgaaatttatccgggaagtttaaaaaatctgatcatttcagcactgaccatttccggccaaaaaacaaatataatggggagagccgttaacagccaaataatagaagaactgtgtcagacaaacaactgcattgttatttctgccagaaattattttcatcgggaatcgcttgtctgccggacatcagtggagggtggggtcatgttatttattcctaaaaaattctttctctgcggcaaacctgatatcaacaggctggccggaacacctgtactttttcatgagggggctaaaaattttaatctggacaccatataccatttttttgaacagacactaggtattaccaaccctgcattcagttttgataacgtcgatttgttcagttcactgtaccggttacaacaagggctggcgatgttactcatccccgtcagagtctgtcgggctctgggattatcaacagatcacgcactgcacatcaaaggcgtagcgctctgtacctccttgtattacccgaccaagaaacgggagacaccagattatcgtaaagctataaaactgatacagcaggaactgaaacagtccaccttctgaccttatgcagcgtaagggccgcaacacctgtattcacggcatttgccagattcagattgtcagcaatccccatcctccatagcggtagttcaccgcggagcatggagtaaaccggctggtcgccgtcaatctgacacagaatcagtttgatgctctggtggattacctaaaacatgggcattaacgcgctggctcacgccactttactgaagaaactgaataacggtgactatgacggcgcagcgaatgaattaattcctgaaatgggaccacgccagcggtcaggttgttcccggcctgacccgacgccggagcgctgaacgttgcttattcctgagttaatttgttgtgccatctttgcacaccgggaaccgcgattccgcacagcagaaaaatagcacataaataaactcaatataagccactcattttctggcaatacaaaataattcccctgcaaacattatcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z