LOCUS       BBa_M1208               2143 bp    DNA     linear       22-Dec-2024
DEFINITION  Kill gene intermediate - Holin/Autolysin portion
ACCESSION   BBa_M1208
VERSION     BBa_M1208.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..74
                     /label=BBa_J61102
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     CDS             825..1481
                     /label=T4 holin
     CDS             809..1484
                     /label=BBa_K112805
     CDS             1509..2003
                     /label=T4 Endolysin
     CDS             1493..2006
                     /label=BBa_K112806
     stem_loop       2026..2069
                     /label=stem_loop
     misc_feature    2015..2094
                     /label=BBa_B0010
     terminator      2015..2094
                     /label=BBa_B0010
     stem_loop       2110..2129
                     /label=T7 TE
     misc_feature    2136..2136
                     /label=stop
     polyA_site      2130..2143
                     /label=polya
     misc_feature    2103..2143
                     /label=BBa_B0012
     terminator      2103..2143
                     /label=BBa_B0012
     terminator      2015..2143
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagatcc gatgtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagct taaaaggagg gtctatggca gcacctagaa
      841 tatcattttc gccctctgat attctatttg gtgttctcga tcgcttgttc aaagataacg
      901 ctaccgggaa ggttcttgct tcccgggtag ctgtcgtaat tcttttgttt ataatggcga
      961 ttgtttggta taggggagat agtttctttg agtactataa gcaatcaaag tatgaaacat
     1021 acagtgaaat tattgaaaag gaaagaactg cacgctttga atctgtcgcc ctggaacaac
     1081 tccagatagt tcatatatca tctgaggcag actttagtgc ggtgtattct ttccgcccta
     1141 aaaacttaaa ctattttgtt gatattatag catacgaagg aaaattacct tcaacaataa
     1201 gtgaaaaatc acttggagga tatcctgttg ataaaactat ggatgaatat acagttcatt
     1261 taaatggacg tcattattat tccaactcaa aatttgcttt tttaccaact aaaaagccta
     1321 ctcccgaaat aaactacatg tacagttgtc catattttaa tttggataat atctatgctg
     1381 gaacgataac catgtactgg tatagaaatg atcatataag taatgaccgc cttgaatcaa
     1441 tatgtgctca ggcggccaga atattaggaa gggctaaata attatactag agatacttag
     1501 gaggtattat gaatatattt gaaatgttac gtatagatga aggtcttaga cttaaaatct
     1561 ataaagacac agaaggctat tacactattg gcatcggtca tttgcttaca aaaagtccat
     1621 cacttaatgc tgctaaatct gaattagata aagctattgg gcgtaattgc aatggtgtaa
     1681 ttacaaaaga tgaggctgaa aaactcttta atcaggatgt tgatgctgct gttcgcggaa
     1741 tcctgagaaa tgctaaatta aaaccggttt atgattctct tgatgcggtt cgtcgctgtg
     1801 cattgattaa tatggttttc caaatgggag aaaccggtgt ggcaggattt actaactctt
     1861 tacgtatgct tcaacaaaaa cgctgggatg aagcagcagt taacttagct aaaagtagat
     1921 ggtataatca aacacctaat cgcgcaaaac gagtcattac aacgtttaga actggcactt
     1981 gggacgcgta taaaaatcta taaagctact agagccaggc atcaaataaa acgaaaggct
     2041 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2101 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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