LOCUS       BBa_M1210               3394 bp    DNA     linear       04-May-2024
DEFINITION  Tet Repressor/Promoter with ROSE Regulation and Kill Gene
ACCESSION   BBa_M1210
VERSION     BBa_M1210.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     stem_loop       705..748
                     /label=stem_loop
     misc_feature    694..773
                     /label=BBa_B0010
     terminator      694..773
                     /label=BBa_B0010
     misc_feature    782..800
                     /label=TetR 1
     misc_feature    782..835
                     /label=BBa_R0040
     promoter        801..806
                     /label=-35
     misc_feature    807..825
                     /label=TetR 2
     promoter        824..829
                     /label=-10
     promoter        782..835
                     /label=BBa_R0040
     RBS             844..855
                     /label=BBa_J61102
     CDS             862..1581
                     /label=GFP protein
     CDS             862..1581
                     /label=BBa_E0040
     CDS             1606..2262
                     /label=T4 holin
     CDS             1590..2265
                     /label=BBa_K112805
     CDS             2290..2784
                     /label=T4 Endolysin
     CDS             2274..2787
                     /label=BBa_K112806
     stem_loop       2807..2850
                     /label=stem_loop
     misc_feature    2796..2875
                     /label=BBa_B0010
     terminator      2796..2875
                     /label=BBa_B0010
     stem_loop       2891..2910
                     /label=T7 TE
     misc_feature    2917..2917
                     /label=stop
     polyA_site      2911..2924
                     /label=polya
     misc_feature    2884..2924
                     /label=BBa_B0012
     terminator      2884..2924
                     /label=BBa_B0012
     terminator      2796..2924
                     /label=BBa_B0015
     promoter        2933..2967
                     /label=BBa_J23116
     RBS             2976..2987
                     /label=BBa_J61101
     CDS             3010..3303
                     /label=T4 antiholin
     CDS             2996..3306
                     /label=BBa_K112807
     stem_loop       3326..3369
                     /label=stem_loop
     misc_feature    3315..3394
                     /label=BBa_B0010
     terminator      3315..3394
                     /label=BBa_B0010
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc
      721 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      781 gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta
      841 gagaaagatc cgatgtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      901 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      961 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1021 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1081 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1141 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1201 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1261 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1321 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1381 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1441 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1501 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1561 gatgaactat acaaataata atactagagc ttaaaaggag ggtctatggc agcacctaga
     1621 atatcatttt cgccctctga tattctattt ggtgttctcg atcgcttgtt caaagataac
     1681 gctaccggga aggttcttgc ttcccgggta gctgtcgtaa ttcttttgtt tataatggcg
     1741 attgtttggt ataggggaga tagtttcttt gagtactata agcaatcaaa gtatgaaaca
     1801 tacagtgaaa ttattgaaaa ggaaagaact gcacgctttg aatctgtcgc cctggaacaa
     1861 ctccagatag ttcatatatc atctgaggca gactttagtg cggtgtattc tttccgccct
     1921 aaaaacttaa actattttgt tgatattata gcatacgaag gaaaattacc ttcaacaata
     1981 agtgaaaaat cacttggagg atatcctgtt gataaaacta tggatgaata tacagttcat
     2041 ttaaatggac gtcattatta ttccaactca aaatttgctt ttttaccaac taaaaagcct
     2101 actcccgaaa taaactacat gtacagttgt ccatatttta atttggataa tatctatgct
     2161 ggaacgataa ccatgtactg gtatagaaat gatcatataa gtaatgaccg ccttgaatca
     2221 atatgtgctc aggcggccag aatattagga agggctaaat aattatacta gagatactta
     2281 ggaggtatta tgaatatatt tgaaatgtta cgtatagatg aaggtcttag acttaaaatc
     2341 tataaagaca cagaaggcta ttacactatt ggcatcggtc atttgcttac aaaaagtcca
     2401 tcacttaatg ctgctaaatc tgaattagat aaagctattg ggcgtaattg caatggtgta
     2461 attacaaaag atgaggctga aaaactcttt aatcaggatg ttgatgctgc tgttcgcgga
     2521 atcctgagaa atgctaaatt aaaaccggtt tatgattctc ttgatgcggt tcgtcgctgt
     2581 gcattgatta atatggtttt ccaaatggga gaaaccggtg tggcaggatt tactaactct
     2641 ttacgtatgc ttcaacaaaa acgctgggat gaagcagcag ttaacttagc taaaagtaga
     2701 tggtataatc aaacacctaa tcgcgcaaaa cgagtcatta caacgtttag aactggcact
     2761 tgggacgcgt ataaaaatct ataaagctac tagagccagg catcaaataa aacgaaaggc
     2821 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2881 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agttgacagc
     2941 tagctcagtc ctagggacta tgctagctac tagagaaaga caggacctac tagaggatag
     3001 gaggccttta tggccttaaa agcaacagca ctttttgcca tgctaggatt gtcatttgtt
     3061 ttatctccat cgattgaagc gaatgtcgat cctcattttg ataaatttat ggaatctggt
     3121 attaggcacg tttatatgct ttttgaaaat aaaagcgtag aatcgtctga acaattctat
     3181 agttttatga gaacgaccta taaaaatgac ccgtgctctt ctgattttga atgtatagag
     3241 cgaggcgcgg agatggcaca atcatacgct agaattatga acattaaatt ggagactgaa
     3301 tgaaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3361 tcgttttatc tgttgtttgt cggtgaacgc tctc
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