BBa_M12101 1 BBa_M12101 PTet : B0034 : GFP in BioScaffold Form, a [0,5;7,2) BioScaffold 2010-11-24T12:00:00Z 2015-05-08T01:13:55Z This is a modified version of the BBa_I13522 part and contains a truncated terminator primarily for ease of use with BioScaffold-oriented priming and assembly. This is a test part for validation of a BioScaffold project. The part is a green fluorescent protein constitutively expressed by the PTet promoter and the strong Elowitz ribosome binding site. false false _548_ 0 6719 9 Not in stock false See documentation about BioScaffold restriction sites- specifically FalI and PsrI- for more information about the requirements for BioScaffold designs. false Grant Robinson annotation2114830 1 Truncated Terminator range2114830 1 827 876 annotation2115225 1 FalI range2115225 1 7 17 annotation2115226 1 FalI range2115226 1 859 870 annotation2114827 1 GFP range2114827 1 99 818 annotation2114829 1 Right BioScaffold Padding range2114829 1 860 876 annotation2114828 1 Left BioScaffold Padding range2114828 1 1 18 annotation2114826 1 Tet Promoter range2114826 1 19 72 annotation2114825 1 Elowitz RBS range2114825 1 81 92 BBa_M12101_sequence 1 cttcttcaagctctcctttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggcttgaacca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z