LOCUS       BBa_M1211               2599 bp    DNA     linear       22-Dec-2024
DEFINITION  Kill Gene with RFP alternative
ACCESSION   BBa_M1211
VERSION     BBa_M1211.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..74
                     /label=BBa_J61102
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..786
                     /label=BBa_E1010
     CDS             811..1467
                     /label=T4 holin
     CDS             795..1470
                     /label=BBa_K112805
     CDS             1495..1989
                     /label=T4 Endolysin
     CDS             1479..1992
                     /label=BBa_K112806
     stem_loop       2012..2055
                     /label=stem_loop
     misc_feature    2001..2080
                     /label=BBa_B0010
     terminator      2001..2080
                     /label=BBa_B0010
     stem_loop       2096..2115
                     /label=T7 TE
     misc_feature    2122..2122
                     /label=stop
     polyA_site      2116..2129
                     /label=polya
     misc_feature    2089..2129
                     /label=BBa_B0012
     terminator      2089..2129
                     /label=BBa_B0012
     terminator      2001..2129
                     /label=BBa_B0015
     promoter        2138..2172
                     /label=BBa_J23116
     RBS             2181..2192
                     /label=BBa_J61101
     CDS             2215..2508
                     /label=T4 antiholin
     CDS             2201..2511
                     /label=BBa_K112807
     stem_loop       2531..2574
                     /label=stem_loop
     misc_feature    2520..2599
                     /label=BBa_B0010
     terminator      2520..2599
                     /label=BBa_B0010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagatcc gatgtactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagcttaaa aggagggtct atggcagcac ctagaatatc attttcgccc
      841 tctgatattc tatttggtgt tctcgatcgc ttgttcaaag ataacgctac cgggaaggtt
      901 cttgcttccc gggtagctgt cgtaattctt ttgtttataa tggcgattgt ttggtatagg
      961 ggagatagtt tctttgagta ctataagcaa tcaaagtatg aaacatacag tgaaattatt
     1021 gaaaaggaaa gaactgcacg ctttgaatct gtcgccctgg aacaactcca gatagttcat
     1081 atatcatctg aggcagactt tagtgcggtg tattctttcc gccctaaaaa cttaaactat
     1141 tttgttgata ttatagcata cgaaggaaaa ttaccttcaa caataagtga aaaatcactt
     1201 ggaggatatc ctgttgataa aactatggat gaatatacag ttcatttaaa tggacgtcat
     1261 tattattcca actcaaaatt tgctttttta ccaactaaaa agcctactcc cgaaataaac
     1321 tacatgtaca gttgtccata ttttaatttg gataatatct atgctggaac gataaccatg
     1381 tactggtata gaaatgatca tataagtaat gaccgccttg aatcaatatg tgctcaggcg
     1441 gccagaatat taggaagggc taaataatta tactagagat acttaggagg tattatgaat
     1501 atatttgaaa tgttacgtat agatgaaggt cttagactta aaatctataa agacacagaa
     1561 ggctattaca ctattggcat cggtcatttg cttacaaaaa gtccatcact taatgctgct
     1621 aaatctgaat tagataaagc tattgggcgt aattgcaatg gtgtaattac aaaagatgag
     1681 gctgaaaaac tctttaatca ggatgttgat gctgctgttc gcggaatcct gagaaatgct
     1741 aaattaaaac cggtttatga ttctcttgat gcggttcgtc gctgtgcatt gattaatatg
     1801 gttttccaaa tgggagaaac cggtgtggca ggatttacta actctttacg tatgcttcaa
     1861 caaaaacgct gggatgaagc agcagttaac ttagctaaaa gtagatggta taatcaaaca
     1921 cctaatcgcg caaaacgagt cattacaacg tttagaactg gcacttggga cgcgtataaa
     1981 aatctataaa gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2041 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2101 accttcgggt gggcctttct gcgtttatat actagagttg acagctagct cagtcctagg
     2161 gactatgcta gctactagag aaagacagga cctactagag gataggaggc ctttatggcc
     2221 ttaaaagcaa cagcactttt tgccatgcta ggattgtcat ttgttttatc tccatcgatt
     2281 gaagcgaatg tcgatcctca ttttgataaa tttatggaat ctggtattag gcacgtttat
     2341 atgctttttg aaaataaaag cgtagaatcg tctgaacaat tctatagttt tatgagaacg
     2401 acctataaaa atgacccgtg ctcttctgat tttgaatgta tagagcgagg cgcggagatg
     2461 gcacaatcat acgctagaat tatgaacatt aaattggaga ctgaatgaaa ttactagagc
     2521 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2581 tttgtcggtg aacgctctc
//