BBa_M1211
type: ComponentDefinition
persistent identity: BBa M1211
display id: BBa_M1211
version: 1
was generated by: iGEM to SBOL conversion
description: Kill Gene with RFP alternative
created: 2011-04-24T11:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: The holin used in the kill gene comes from a bacteriophage.
owned by: james
owned by: myers
mutable description: This part contains a kill gene that uses holin to produce holes in the cell wall and destroy the cell if the cell is below 42 degrees Celsius or in the presence of tetracycline. RFP is used as a reporter to visually alert the researcher to a cell which is marked for death.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This gene was designed to allow for expression of the reporter gene previous to the expression of the kill gene.
star: false
creator: Lindsey Stevens
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_K112807
component: BBa_J61101
component: BBa_J23116
component: BBa_B0015
component: BBa_K112806
component: BBa_K112805
component: BBa_E1010
component: BBa_J61102
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J61102
sequence annotation: BBa_E1010
sequence annotation: BBa_K112805
sequence annotation: BBa_K112806
sequence annotation: BBa_B0015
sequence annotation: BBa_J23116
sequence annotation: BBa_J61101
sequence annotation: BBa_K112807
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_M1211_sequence/1

BBa_B0010
type: Component
persistent identity: component2295424
display id: component2295424
version: 1
top level: BBa_M1211
definition: BBa_B0010
access: public

BBa_K112807
type: Component
persistent identity: component2295423
display id: component2295423
version: 1
top level: BBa_M1211
definition: BBa_K112807
access: public

BBa_J61101
type: Component
persistent identity: component2295421
display id: component2295421
version: 1
top level: BBa_M1211
definition: BBa_J61101
access: public

BBa_J23116
type: Component
persistent identity: component2295420
display id: component2295420
version: 1
top level: BBa_M1211
definition: BBa_J23116
access: public

BBa_B0015
type: Component
persistent identity: component2295419
display id: component2295419
version: 1
top level: BBa_M1211
definition: BBa_B0015
access: public

BBa_K112806
type: Component
persistent identity: component2295412
display id: component2295412
version: 1
top level: BBa_M1211
definition: BBa_K112806
access: public

BBa_K112805
type: Component
persistent identity: component2295410
display id: component2295410
version: 1
top level: BBa_M1211
definition: BBa_K112805
access: public

BBa_E1010
type: Component
persistent identity: component2295408
display id: component2295408
version: 1
top level: BBa_M1211
definition: mRFP1
access: public

BBa_J61102
type: Component
persistent identity: component2295405
display id: component2295405
version: 1
top level: BBa_M1211
definition: BBa_J61102
access: public

BBa_R0040
type: Component
persistent identity: component2295400
display id: component2295400
version: 1
top level: BBa_M1211
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2295400
display id: annotation2295400
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295400/range2295400/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M1211/annotation2295400/range2295400/1
type: Range
persistent identity: range2295400
display id: range2295400
version: 1
top level: BBa_M1211
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J61102
type: SequenceAnnotation
persistent identity: annotation2295405
display id: annotation2295405
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295405/range2295405/1
component: BBa_J61102

https://synbiohub.org/public/igem/BBa_M1211/annotation2295405/range2295405/1
type: Range
persistent identity: range2295405
display id: range2295405
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2295408
display id: annotation2295408
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295408/range2295408/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M1211/annotation2295408/range2295408/1
type: Range
persistent identity: range2295408
display id: range2295408
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 81
end: 786
orientation: inline

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2295410
display id: annotation2295410
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295410/range2295410/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_M1211/annotation2295410/range2295410/1
type: Range
persistent identity: range2295410
display id: range2295410
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 795
end: 1470
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2295412
display id: annotation2295412
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295412/range2295412/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_M1211/annotation2295412/range2295412/1
type: Range
persistent identity: range2295412
display id: range2295412
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 1479
end: 1992
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295419
display id: annotation2295419
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295419/range2295419/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1211/annotation2295419/range2295419/1
type: Range
persistent identity: range2295419
display id: range2295419
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 2001
end: 2129
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2295420
display id: annotation2295420
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295420/range2295420/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_M1211/annotation2295420/range2295420/1
type: Range
persistent identity: range2295420
display id: range2295420
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 2138
end: 2172
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2295421
display id: annotation2295421
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295421/range2295421/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_M1211/annotation2295421/range2295421/1
type: Range
persistent identity: range2295421
display id: range2295421
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 2181
end: 2192
orientation: inline

BBa_K112807
type: SequenceAnnotation
persistent identity: annotation2295423
display id: annotation2295423
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295423/range2295423/1
component: BBa_K112807

https://synbiohub.org/public/igem/BBa_M1211/annotation2295423/range2295423/1
type: Range
persistent identity: range2295423
display id: range2295423
version: 1
top level: BBa_M1211
direction: direction/reverse
start: 2201
end: 2511
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2295424
display id: annotation2295424
version: 1
top level: BBa_M1211
location: https://synbiohub.org/public/igem/BBa_M1211/annotation2295424/range2295424/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M1211/annotation2295424/range2295424/1
type: Range
persistent identity: range2295424
display id: range2295424
version: 1
top level: BBa_M1211
direction: direction/forward
start: 2520
end: 2599
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: fix
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_J61102
type: ComponentDefinition
persistent identity: BBa J61102
display id: BBa_J61102
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61102_sequence/1

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

BBa_K112807
type: ComponentDefinition
persistent identity: BBa K112807
display id: BBa_K112807
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 antiholin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
owned by: james
owned by: myers
mutable description: Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 antiholin
sequence: https://synbiohub.org/public/igem/BBa_K112807_sequence/1

T4 antiholin
type: SequenceAnnotation
persistent identity: annotation1999019
display id: annotation1999019
version: 1
top level: BBa_K112807
location: https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
type: Range
persistent identity: range1999019
display id: range1999019
version: 1
top level: BBa_K112807
direction: direction/forward
start: 15
end: 308
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_M1211_sequence/1
type: Sequence
persistent identity: BBa M1211 sequence
display id: BBa_M1211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagatccgatgtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61101_sequence/1
type: Sequence
persistent identity: BBa J61101 sequence
display id: BBa_J61101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagacaggacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61102_sequence/1
type: Sequence
persistent identity: BBa J61102 sequence
display id: BBa_J61102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagatccgatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112807_sequence/1
type: Sequence
persistent identity: BBa K112807 sequence
display id: BBa_K112807_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z