LOCUS       BBa_M1212               2616 bp    DNA     linear       22-Nov-2024
DEFINITION  Kill Gene with CFP alternative
ACCESSION   BBa_M1212
VERSION     BBa_M1212.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..74
                     /label=BBa_J61102
     CDS             81..803
                     /label=BBa_E0020
     CDS             828..1484
                     /label=T4 holin
     CDS             812..1487
                     /label=BBa_K112805
     CDS             1512..2006
                     /label=T4 Endolysin
     CDS             1496..2009
                     /label=BBa_K112806
     stem_loop       2029..2072
                     /label=stem_loop
     misc_feature    2018..2097
                     /label=BBa_B0010
     terminator      2018..2097
                     /label=BBa_B0010
     stem_loop       2113..2132
                     /label=T7 TE
     misc_feature    2139..2139
                     /label=stop
     polyA_site      2133..2146
                     /label=polya
     misc_feature    2106..2146
                     /label=BBa_B0012
     terminator      2106..2146
                     /label=BBa_B0012
     terminator      2018..2146
                     /label=BBa_B0015
     promoter        2155..2189
                     /label=BBa_J23116
     RBS             2198..2209
                     /label=BBa_J61101
     CDS             2232..2525
                     /label=T4 antiholin
     CDS             2218..2528
                     /label=BBa_K112807
     stem_loop       2548..2591
                     /label=stem_loop
     misc_feature    2537..2616
                     /label=BBa_B0010
     terminator      2537..2616
                     /label=BBa_B0010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagatcc gatgtactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagtaa taatactaga gcttaaaagg agggtctatg gcagcaccta
      841 gaatatcatt ttcgccctct gatattctat ttggtgttct cgatcgcttg ttcaaagata
      901 acgctaccgg gaaggttctt gcttcccggg tagctgtcgt aattcttttg tttataatgg
      961 cgattgtttg gtatagggga gatagtttct ttgagtacta taagcaatca aagtatgaaa
     1021 catacagtga aattattgaa aaggaaagaa ctgcacgctt tgaatctgtc gccctggaac
     1081 aactccagat agttcatata tcatctgagg cagactttag tgcggtgtat tctttccgcc
     1141 ctaaaaactt aaactatttt gttgatatta tagcatacga aggaaaatta ccttcaacaa
     1201 taagtgaaaa atcacttgga ggatatcctg ttgataaaac tatggatgaa tatacagttc
     1261 atttaaatgg acgtcattat tattccaact caaaatttgc ttttttacca actaaaaagc
     1321 ctactcccga aataaactac atgtacagtt gtccatattt taatttggat aatatctatg
     1381 ctggaacgat aaccatgtac tggtatagaa atgatcatat aagtaatgac cgccttgaat
     1441 caatatgtgc tcaggcggcc agaatattag gaagggctaa ataattatac tagagatact
     1501 taggaggtat tatgaatata tttgaaatgt tacgtataga tgaaggtctt agacttaaaa
     1561 tctataaaga cacagaaggc tattacacta ttggcatcgg tcatttgctt acaaaaagtc
     1621 catcacttaa tgctgctaaa tctgaattag ataaagctat tgggcgtaat tgcaatggtg
     1681 taattacaaa agatgaggct gaaaaactct ttaatcagga tgttgatgct gctgttcgcg
     1741 gaatcctgag aaatgctaaa ttaaaaccgg tttatgattc tcttgatgcg gttcgtcgct
     1801 gtgcattgat taatatggtt ttccaaatgg gagaaaccgg tgtggcagga tttactaact
     1861 ctttacgtat gcttcaacaa aaacgctggg atgaagcagc agttaactta gctaaaagta
     1921 gatggtataa tcaaacacct aatcgcgcaa aacgagtcat tacaacgttt agaactggca
     1981 cttgggacgc gtataaaaat ctataaagct actagagcca ggcatcaaat aaaacgaaag
     2041 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2101 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgaca
     2161 gctagctcag tcctagggac tatgctagct actagagaaa gacaggacct actagaggat
     2221 aggaggcctt tatggcctta aaagcaacag cactttttgc catgctagga ttgtcatttg
     2281 ttttatctcc atcgattgaa gcgaatgtcg atcctcattt tgataaattt atggaatctg
     2341 gtattaggca cgtttatatg ctttttgaaa ataaaagcgt agaatcgtct gaacaattct
     2401 atagttttat gagaacgacc tataaaaatg acccgtgctc ttctgatttt gaatgtatag
     2461 agcgaggcgc ggagatggca caatcatacg ctagaattat gaacattaaa ttggagactg
     2521 aatgaaatta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2581 tttcgtttta tctgttgttt gtcggtgaac gctctc
//