LOCUS       BBa_M1271               3189 bp    DNA     linear       09-Jan-2025
DEFINITION  human Kappa Opioid Receptor composite
ACCESSION   BBa_M1271
VERSION     BBa_M1271.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    906..906
                     /label=I281S T->G
     misc_feature    1415..1417
                     /label=TAA
     misc_feature    65..67
                     /label=ATG
     CDS             65..1417
                     /label=BBa_K136046
     RBS             1426..1440
                     /label=RBS-1\Strong
     RBS             1433..1437
                     /label=RBS
     misc_feature    1426..1440
                     /label=BBa_B0030
     RBS             1426..1440
                     /label=BBa_B0030
     CDS             1449..3052
                     /label=BBa_M1269
     stem_loop       3072..3115
                     /label=stem_loop
     misc_feature    3061..3140
                     /label=BBa_B0010
     terminator      3061..3140
                     /label=BBa_B0010
     stem_loop       3156..3175
                     /label=T7 TE
     misc_feature    3182..3182
                     /label=stop
     polyA_site      3176..3189
                     /label=polya
     misc_feature    3149..3189
                     /label=BBa_B0012
     terminator      3149..3189
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgagg cgattgcgct tctcgccacg aagttcattt gcccgtacgt tattgctcat
      121 cgtcaccttg ctgttcgcca gcctggtgac gacttatctg gtggtgctga acttcgcgat
      181 tttgccgagc ctccagcagt ttaataaagt cctcgcgtac gaagtgcgta tgttgatgac
      241 cgacaaactg caactggagg acggcacgca gttggttgtg cctcccgctt tccgtcggga
      301 gatctaccgt gagctgggga tctctctcta ctccaacgag gctgccgaag aggcaggtct
      361 gcgttgggcg caacactatg aattcttaag ccatcagatg gcgcagcaac tgggcggccc
      421 gacggaagtg cgcgttgagg tcaacaaaag ttcgcctgtc gtctggctga aaacctggct
      481 gtcgcccaat atctgggtac gcgtgccgct gaccgaaatt catcagggcg atttctctcc
      541 gctgttccgc tatacgctgg cgattatgct attggcgata ggcggggcgt ggctgtttat
      601 tcgtatccag aaccgaccgt tggtcgatct cgaacacgca gccttgcagg ttggtaaagg
      661 gattattccg ccgccgctgc gtgagtatgg cgcttcggag gtgcgttccg ttacccgtgc
      721 ctttaaccat atggcggctg gtgttaagca actggcggat gaccgcacgc tgctgatggc
      781 gggggtaagt cacgacttgc gcacgccgct gacgcgtatt cgcctggcga ctgagatgat
      841 gagcgagcag gatggctatc tggcagaatc gatcaataaa gatatcgaag agtgcaacgc
      901 catcagtgag cagtttatcg actacctgcg caccgggcag gagatgccga tggaaatggc
      961 ggatcttaat gcagtactcg gtgaggtgat tgctgccgaa agtggctatg agcgggaaat
     1021 tgaaaccgcg ctttaccccg gcagcattga agtgaaaatg cacccgctgt cgatcaaacg
     1081 cgcggtggcg aatatggtgg tcaacgccgc ccgttatggc aatggctgga tcaaagtcag
     1141 cagcggaacg gagccgaatc gcgcctggtt ccaggtggaa gatgacggtc cgggaattgc
     1201 gccggaacaa cgtaagcacc tgttccagcc gtttgtccgc ggcgacagtg cgcgcaccat
     1261 tagcggcacg ggattagggc tggcaattgt gcagcgtatc gtggataacc ataacgggat
     1321 gctggagctt ggcaccagcg agcggggcgg gctttccatt cgcgcctggc tgccagtgcc
     1381 ggtaacgcgg gcgcagggca cgacaaaaga agggtaatac tagagattaa agaggagaaa
     1441 tactagagga gctccgtgct gggaggtggg aagggggctt gaccctgggg actcaggcag
     1501 tctggggaca gttccaccag gggccggtgc ctaagaattg gtgagggagg cacctcaggg
     1561 gcttggggga gaaggaacga gcgctcttcg cccctctctg gcacccagcg gcgcgcctgc
     1621 tggccggaaa ggcagcgaga agtccgttct ccctgtcctg cccccggcga cttgcggccc
     1681 gggtgggagt ccgcaggctc cgggtcccca gcgccgctgg ccagggcgcg ggcaaagttt
     1741 gcctctccgc gtccagccgg ttctttgcct cccgcagcgc cgcaggtgcc gcctgtcctc
     1801 gccttcctgc tgcaatcgcc ccaccatgga ctccccgatc cagatcttcc gcggggagcc
     1861 gggccctacc tgcgccccga gcgcctgcct gccccccaac agcagcgcct ggtttcccgg
     1921 ctgggccgag cccgacagca acggcagcgc cggctcgcag gacgcgcagc tggagcccgc
     1981 gcacatctcc ccggccatcc cggtcatcat cacggccgtc tactccgtag tgttcgtcgt
     2041 gggcttggtg ggcaactcgc tggtcatgtt cgtgatcatc cgatacacaa agatgaagac
     2101 agcaaccaac atttacatat ttaacctggc tttggcagat gctttagtta ctacaaccat
     2161 gccctttcag agtacggtct acttgatgaa ttcctggcct tttggggatg tgctgtgcaa
     2221 gatagtaatt tccattgatt actacaacat gttcaccagc atcttcacct tgaccatgat
     2281 gagcgtggac cgctacattg ccgtgtgcca ccccgtgaag gctttggact tccgcacacc
     2341 cttgaaggca aagatcatca atatctgcat ctggctgctg tcgtcatctg ttggcatctc
     2401 tgcaatagtc cttggagggc accaaagtca gggaagactc gatgtcattg agtgctcctt
     2461 gcagttccca gatgatgact actcctggtg ggacctcttc atgaagatct gcgtcttcat
     2521 ctttgccttc gtgatccctg tcctcatcat catcgtctgc tacaccctga tgatcctgcg
     2581 tctcaagagc gtccggctcc tttctggctc ccgagagaaa gatcgcaacc tgcgtaggat
     2641 caccagactg gtcctggtgg tggtggcagt cttcgtcgtc tgctggactc ccattcacat
     2701 attcatcctg gtggaggctc tggggagcac ctcccacagc acagctgctc tctccagcta
     2761 ttacttctgc atcgccttag gctataccaa cagtagcctg aatcccattc tctacgcctt
     2821 tcttgatgaa aacttcaagc ggtgtttccg ggacttctgc tttccactga agatgaggat
     2881 ggagcggcag agcactagca gagtccgaaa tacagttcag gatcctgctt acctgaggga
     2941 catcgatggg atgaataaac cagtatgact agtcgtggag atgtcttcgt acagttcttc
     3001 gggaagagag gagttcaatg atctaggttt aactcagatc actactgcag tctactagag
     3061 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3121 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     3181 gcgtttata
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