##gff-version 3
##sequence-region BBa_M1271 1 3189
BBa_M1271	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_M1271	.	CDS	1449	3052	.	+	0	ID=BBa_M1269;Name=BBa_M1269
BBa_M1271	.	ribosome_entry_site	1426	1440	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1271	.	ribosome_entry_site	1426	1440	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1271	.	engineered_region	1426	1440	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1271	.	ribosome_entry_site	1433	1437	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1271	.	terminator	3061	3140	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1271	.	engineered_region	3061	3140	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1271	.	stem_loop	3072	3115	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1271	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1271	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1271	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1271	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1271	.	CDS	65	1417	.	+	0	ID=BBa_K136046;Name=BBa_K136046
BBa_M1271	.	start_codon	65	67	.	+	0	ID=annotation1994304;Name=ATG;Parent=BBa_K136046
BBa_M1271	.	point_mutation	906	906	.	+	0	ID=annotation1994273;Name=I281S T->G;Parent=BBa_K136046
BBa_M1271	.	stop_codon	1415	1417	.	+	0	ID=annotation1994303;Name=TAA;Parent=BBa_K136046
BBa_M1271	.	terminator	3149	3189	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1271	.	stem_loop	3156	3175	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1271	.	polyA_site	3176	3189	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1271	.	engineered_region	3149	3189	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1271	.	stop_codon	3182	3182	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_M1271
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgc
ttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatc
tggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgat
gaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctg
gggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatc
agatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaac
ctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctat
acgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcg
aacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgt
tacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagt
cacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaat
cgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgcc
gatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaacc
gcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacg
ccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatga
cggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagc
ggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagc
ggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatacta
gagattaaagaggagaaatactagaggagctccgtgctgggaggtgggaagggggcttgaccctggggactcaggcagt
ctggggacagttccaccaggggccggtgcctaagaattggtgagggaggcacctcaggggcttgggggagaaggaacga
gcgctcttcgcccctctctggcacccagcggcgcgcctgctggccggaaaggcagcgagaagtccgttctccctgtcct
gcccccggcgacttgcggcccgggtgggagtccgcaggctccgggtccccagcgccgctggccagggcgcgggcaaagt
ttgcctctccgcgtccagccggttctttgcctcccgcagcgccgcaggtgccgcctgtcctcgccttcctgctgcaatc
gccccaccatggactccccgatccagatcttccgcggggagccgggccctacctgcgccccgagcgcctgcctgccccc
caacagcagcgcctggtttcccggctgggccgagcccgacagcaacggcagcgccggctcgcaggacgcgcagctggag
cccgcgcacatctccccggccatcccggtcatcatcacggccgtctactccgtagtgttcgtcgtgggcttggtgggca
actcgctggtcatgttcgtgatcatccgatacacaaagatgaagacagcaaccaacatttacatatttaacctggcttt
ggcagatgctttagttactacaaccatgccctttcagagtacggtctacttgatgaattcctggccttttggggatgtg
ctgtgcaagatagtaatttccattgattactacaacatgttcaccagcatcttcaccttgaccatgatgagcgtggacc
gctacattgccgtgtgccaccccgtgaaggctttggacttccgcacacccttgaaggcaaagatcatcaatatctgcat
ctggctgctgtcgtcatctgttggcatctctgcaatagtccttggagggcaccaaagtcagggaagactcgatgtcatt
gagtgctccttgcagttcccagatgatgactactcctggtgggacctcttcatgaagatctgcgtcttcatctttgcct
tcgtgatccctgtcctcatcatcatcgtctgctacaccctgatgatcctgcgtctcaagagcgtccggctcctttctgg
ctcccgagagaaagatcgcaacctgcgtaggatcaccagactggtcctggtggtggtggcagtcttcgtcgtctgctgg
actcccattcacatattcatcctggtggaggctctggggagcacctcccacagcacagctgctctctccagctattact
tctgcatcgccttaggctataccaacagtagcctgaatcccattctctacgcctttcttgatgaaaacttcaagcggtg
tttccgggacttctgctttccactgaagatgaggatggagcggcagagcactagcagagtccgaaatacagttcaggat
cctgcttacctgagggacatcgatgggatgaataaaccagtatgactagtcgtggagatgtcttcgtacagttcttcgg
gaagagaggagttcaatgatctaggtttaactcagatcactactgcagtctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata