BBa_M1307
type: ComponentDefinition
persistent identity: BBa M1307
display id: BBa_M1307
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 genome
created: 2007-02-12T12:00:00Z
modified: 2015-05-08T01:13:55Z
mutable provenance: sequenced in 2002 by L. Panganaban. sequence downloaded from New England Biolabs.
owned by: james
owned by: myers
mutable description: M13 is a filamentous phage that infects E. coli. It packages a single stranded genome of ~6400 bases, encoding 11 ORFs. The M13K07 sequence entered here includes the entire M13 genome as well as a sequence from pACYC177 bearing the p15a bacterial origin of replication and a kanamycin resistance gene. These additional sequences were inserted into the packaging sequence of M13, enabling the phage to propogate as a Kan-selectable plasmid. The modification does attenuate phage production though plaques are still detectable on NEB strain ER2267 (genotype: : F?? proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10). In the presence of a second plasmid bearing the M13 packaging sequence and origin, M13K07 serves as a "helper phage," providing the proteins for the phage coat but allowing the alternative plasmid to be preferentially placed in the phage capsid.
discontinued: false
dominant: true
group u list: _45_102_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Natalie Kuldell
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: gene 2
sequence annotation: promoter-g10
sequence annotation: rbs-g10
sequence annotation: gene 10
sequence annotation: promoter g5
sequence annotation: rbs-g5
sequence annotation: g5 (ORF)
sequence annotation: BsrGI
sequence annotation: rbs-g7
sequence annotation: promoter-g8
sequence annotation: rbs-g9
sequence annotation: gene 9
sequence annotation: SnaBI
sequence annotation: rbs-g8
sequence annotation: gene VIII
sequence annotation: promoter-g3
sequence annotation: rbs-g3
sequence annotation: gene III (ORF)
sequence annotation: BamHI
sequence annotation: promoter-g6
sequence annotation: rbs-g6
sequence annotation: gene VI
sequence annotation: promoter-g1
sequence annotation: g1
sequence annotation: rbs-g11
sequence annotation: promoter-g4
sequence annotation: PacI
sequence annotation: rbs-g4
sequence annotation: rbs g-4
sequence annotation: g4
sequence annotation: ApaLI
sequence annotation: MscI
sequence annotation: NgoMIV
sequence annotation: DrdI
sequence annotation: SgrAI
sequence annotation: SacII
sequence annotation: BssSI
sequence annotation: BstBI
sequence annotation: AhdI
sequence annotation: SacI
sequence annotation: HindIII
sequence annotation: BsmBI
sequence annotation: PvuI
sequence annotation: EcoNI
sequence annotation: NruI
sequence annotation: XhoI
sequence annotation: TliI
sequence annotation: Pst1
sequence annotation: promoter-g2
sequence annotation: SwaI

gene 2
type: SequenceAnnotation
persistent identity: annotation1919704
display id: annotation1919704
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919704/range1919704/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1307/annotation1919704/range1919704/1
type: Range
persistent identity: range1919704
display id: range1919704
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1
end: 831
orientation: inline

promoter-g10
type: SequenceAnnotation
persistent identity: annotation1919707
display id: annotation1919707
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919707/range1919707/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919707/range1919707/1
type: Range
persistent identity: range1919707
display id: range1919707
version: 1
top level: BBa_M1307
direction: direction/forward
start: 381
end: 428
orientation: inline

rbs-g10
type: SequenceAnnotation
persistent identity: annotation1919708
display id: annotation1919708
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919708/range1919708/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919708/range1919708/1
type: Range
persistent identity: range1919708
display id: range1919708
version: 1
top level: BBa_M1307
direction: direction/forward
start: 480
end: 495
orientation: inline

gene 10
type: SequenceAnnotation
persistent identity: annotation1919709
display id: annotation1919709
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919709/range1919709/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1307/annotation1919709/range1919709/1
type: Range
persistent identity: range1919709
display id: range1919709
version: 1
top level: BBa_M1307
direction: direction/forward
start: 496
end: 831
orientation: inline

promoter g5
type: SequenceAnnotation
persistent identity: annotation1919710
display id: annotation1919710
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919710/range1919710/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919710/range1919710/1
type: Range
persistent identity: range1919710
display id: range1919710
version: 1
top level: BBa_M1307
direction: direction/forward
start: 786
end: 835
orientation: inline

rbs-g5
type: SequenceAnnotation
persistent identity: annotation1919711
display id: annotation1919711
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919711/range1919711/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919711/range1919711/1
type: Range
persistent identity: range1919711
display id: range1919711
version: 1
top level: BBa_M1307
direction: direction/forward
start: 827
end: 842
orientation: inline

g5 (ORF)
type: SequenceAnnotation
persistent identity: annotation1919712
display id: annotation1919712
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919712/range1919712/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1307/annotation1919712/range1919712/1
type: Range
persistent identity: range1919712
display id: range1919712
version: 1
top level: BBa_M1307
direction: direction/forward
start: 843
end: 1106
orientation: inline

BsrGI
type: SequenceAnnotation
persistent identity: annotation1919713
display id: annotation1919713
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919713/range1919713/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919713/range1919713/1
type: Range
persistent identity: range1919713
display id: range1919713
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1021
end: 1025
orientation: inline

rbs-g7
type: SequenceAnnotation
persistent identity: annotation1919714
display id: annotation1919714
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919714/range1919714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919714/range1919714/1
type: Range
persistent identity: range1919714
display id: range1919714
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1092
end: 1107
orientation: inline

promoter-g8
type: SequenceAnnotation
persistent identity: annotation1919715
display id: annotation1919715
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919715/range1919715/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919715/range1919715/1
type: Range
persistent identity: range1919715
display id: range1919715
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1155
end: 1201
orientation: inline

rbs-g9
type: SequenceAnnotation
persistent identity: annotation1919716
display id: annotation1919716
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919716/range1919716/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M1307/annotation1919716/range1919716/1
type: Range
persistent identity: range1919716
display id: range1919716
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1190
end: 1205
orientation: inline

gene 9
type: SequenceAnnotation
persistent identity: annotation1919717
display id: annotation1919717
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919717/range1919717/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1307/annotation1919717/range1919717/1
type: Range
persistent identity: range1919717
display id: range1919717
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1206
end: 1304
orientation: inline

SnaBI
type: SequenceAnnotation
persistent identity: annotation1919718
display id: annotation1919718
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919718/range1919718/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919718/range1919718/1
type: Range
persistent identity: range1919718
display id: range1919718
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1268
end: 1273
orientation: inline

rbs-g8
type: SequenceAnnotation
persistent identity: annotation1919719
display id: annotation1919719
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919719/range1919719/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M1307/annotation1919719/range1919719/1
type: Range
persistent identity: range1919719
display id: range1919719
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1285
end: 1300
orientation: inline

gene VIII
type: SequenceAnnotation
persistent identity: annotation1919720
display id: annotation1919720
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919720/range1919720/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1307/annotation1919720/range1919720/1
type: Range
persistent identity: range1919720
display id: range1919720
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1301
end: 1522
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1919721
display id: annotation1919721
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919721/range1919721/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919721/range1919721/1
type: Range
persistent identity: range1919721
display id: range1919721
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1500
end: 1547
orientation: inline

rbs-g3
type: SequenceAnnotation
persistent identity: annotation1919722
display id: annotation1919722
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919722/range1919722/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M1307/annotation1919722/range1919722/1
type: Range
persistent identity: range1919722
display id: range1919722
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1563
end: 1578
orientation: inline

gene III (ORF)
type: SequenceAnnotation
persistent identity: annotation1919723
display id: annotation1919723
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919723/range1919723/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1307/annotation1919723/range1919723/1
type: Range
persistent identity: range1919723
display id: range1919723
version: 1
top level: BBa_M1307
direction: direction/forward
start: 1579
end: 2853
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1919724
display id: annotation1919724
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919724/range1919724/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919724/range1919724/1
type: Range
persistent identity: range1919724
display id: range1919724
version: 1
top level: BBa_M1307
direction: direction/forward
start: 2220
end: 2224
orientation: inline

promoter-g6
type: SequenceAnnotation
persistent identity: annotation1919726
display id: annotation1919726
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919726/range1919726/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919726/range1919726/1
type: Range
persistent identity: range1919726
display id: range1919726
version: 1
top level: BBa_M1307
direction: direction/forward
start: 2716
end: 2764
orientation: inline

promoter-g6
type: SequenceAnnotation
persistent identity: annotation1919725
display id: annotation1919725
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919725/range1919725/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M1307/annotation1919725/range1919725/1
type: Range
persistent identity: range1919725
display id: range1919725
version: 1
top level: BBa_M1307
direction: direction/forward
start: 2716
end: 2764
orientation: inline

rbs-g6
type: SequenceAnnotation
persistent identity: annotation1919727
display id: annotation1919727
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919727/range1919727/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919727/range1919727/1
type: Range
persistent identity: range1919727
display id: range1919727
version: 1
top level: BBa_M1307
direction: direction/forward
start: 2840
end: 2855
orientation: inline

gene VI
type: SequenceAnnotation
persistent identity: annotation1919728
display id: annotation1919728
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919728/range1919728/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1307/annotation1919728/range1919728/1
type: Range
persistent identity: range1919728
display id: range1919728
version: 1
top level: BBa_M1307
direction: direction/forward
start: 2856
end: 3194
orientation: inline

promoter-g1
type: SequenceAnnotation
persistent identity: annotation1919729
display id: annotation1919729
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919729/range1919729/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919729/range1919729/1
type: Range
persistent identity: range1919729
display id: range1919729
version: 1
top level: BBa_M1307
direction: direction/forward
start: 3086
end: 3132
orientation: inline

g1
type: SequenceAnnotation
persistent identity: annotation1919730
display id: annotation1919730
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919730/range1919730/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1307/annotation1919730/range1919730/1
type: Range
persistent identity: range1919730
display id: range1919730
version: 1
top level: BBa_M1307
direction: direction/forward
start: 3196
end: 4242
orientation: inline

rbs-g11
type: SequenceAnnotation
persistent identity: annotation1919731
display id: annotation1919731
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919731/range1919731/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M1307/annotation1919731/range1919731/1
type: Range
persistent identity: range1919731
display id: range1919731
version: 1
top level: BBa_M1307
direction: direction/forward
start: 3900
end: 3915
orientation: inline

promoter-g4
type: SequenceAnnotation
persistent identity: annotation1919732
display id: annotation1919732
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919732/range1919732/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M1307/annotation1919732/range1919732/1
type: Range
persistent identity: range1919732
display id: range1919732
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4055
end: 4103
orientation: inline

PacI
type: SequenceAnnotation
persistent identity: annotation1919733
display id: annotation1919733
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919733/range1919733/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919733/range1919733/1
type: Range
persistent identity: range1919733
display id: range1919733
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4131
end: 4138
orientation: inline

rbs-g4
type: SequenceAnnotation
persistent identity: annotation1919734
display id: annotation1919734
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919734/range1919734/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919734/range1919734/1
type: Range
persistent identity: range1919734
display id: range1919734
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4204
end: 4219
orientation: inline

rbs g-4
type: SequenceAnnotation
persistent identity: annotation1919735
display id: annotation1919735
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919735/range1919735/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919735/range1919735/1
type: Range
persistent identity: range1919735
display id: range1919735
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4204
end: 4219
orientation: inline

g4
type: SequenceAnnotation
persistent identity: annotation1919736
display id: annotation1919736
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919736/range1919736/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M1307/annotation1919736/range1919736/1
type: Range
persistent identity: range1919736
display id: range1919736
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4220
end: 5500
orientation: inline

ApaLI
type: SequenceAnnotation
persistent identity: annotation1919737
display id: annotation1919737
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919737/range1919737/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919737/range1919737/1
type: Range
persistent identity: range1919737
display id: range1919737
version: 1
top level: BBa_M1307
direction: direction/forward
start: 4743
end: 4748
orientation: inline

MscI
type: SequenceAnnotation
persistent identity: annotation1919738
display id: annotation1919738
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919738/range1919738/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919738/range1919738/1
type: Range
persistent identity: range1919738
display id: range1919738
version: 1
top level: BBa_M1307
direction: direction/forward
start: 5080
end: 5085
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation1919739
display id: annotation1919739
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919739/range1919739/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919739/range1919739/1
type: Range
persistent identity: range1919739
display id: range1919739
version: 1
top level: BBa_M1307
direction: direction/forward
start: 5613
end: 5617
orientation: inline

DrdI
type: SequenceAnnotation
persistent identity: annotation1919740
display id: annotation1919740
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919740/range1919740/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919740/range1919740/1
type: Range
persistent identity: range1919740
display id: range1919740
version: 1
top level: BBa_M1307
direction: direction/forward
start: 5763
end: 5765
orientation: inline

SgrAI
type: SequenceAnnotation
persistent identity: annotation1919741
display id: annotation1919741
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919741/range1919741/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919741/range1919741/1
type: Range
persistent identity: range1919741
display id: range1919741
version: 1
top level: BBa_M1307
direction: direction/forward
start: 5867
end: 5871
orientation: inline

SacII
type: SequenceAnnotation
persistent identity: annotation1919742
display id: annotation1919742
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919742/range1919742/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919742/range1919742/1
type: Range
persistent identity: range1919742
display id: range1919742
version: 1
top level: BBa_M1307
direction: direction/forward
start: 6030
end: 6035
orientation: inline

BssSI
type: SequenceAnnotation
persistent identity: annotation1919743
display id: annotation1919743
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919743/range1919743/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919743/range1919743/1
type: Range
persistent identity: range1919743
display id: range1919743
version: 1
top level: BBa_M1307
direction: direction/forward
start: 6158
end: 6162
orientation: inline

BstBI
type: SequenceAnnotation
persistent identity: annotation1919744
display id: annotation1919744
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919744/range1919744/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919744/range1919744/1
type: Range
persistent identity: range1919744
display id: range1919744
version: 1
top level: BBa_M1307
direction: direction/forward
start: 6518
end: 6520
orientation: inline

AhdI
type: SequenceAnnotation
persistent identity: annotation1919745
display id: annotation1919745
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919745/range1919745/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919745/range1919745/1
type: Range
persistent identity: range1919745
display id: range1919745
version: 1
top level: BBa_M1307
direction: direction/forward
start: 6824
end: 6834
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1919746
display id: annotation1919746
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919746/range1919746/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919746/range1919746/1
type: Range
persistent identity: range1919746
display id: range1919746
version: 1
top level: BBa_M1307
direction: direction/forward
start: 6951
end: 6956
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1919748
display id: annotation1919748
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919748/range1919748/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919748/range1919748/1
type: Range
persistent identity: range1919748
display id: range1919748
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7402
end: 7407
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1919747
display id: annotation1919747
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919747/range1919747/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919747/range1919747/1
type: Range
persistent identity: range1919747
display id: range1919747
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7402
end: 7406
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation1919749
display id: annotation1919749
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919749/range1919749/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919749/range1919749/1
type: Range
persistent identity: range1919749
display id: range1919749
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7504
end: 7508
orientation: inline

PvuI
type: SequenceAnnotation
persistent identity: annotation1919750
display id: annotation1919750
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919750/range1919750/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919750/range1919750/1
type: Range
persistent identity: range1919750
display id: range1919750
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7524
end: 7526
orientation: inline

EcoNI
type: SequenceAnnotation
persistent identity: annotation1919751
display id: annotation1919751
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919751/range1919751/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919751/range1919751/1
type: Range
persistent identity: range1919751
display id: range1919751
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7611
end: 7612
orientation: inline

NruI
type: SequenceAnnotation
persistent identity: annotation1919752
display id: annotation1919752
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919752/range1919752/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919752/range1919752/1
type: Range
persistent identity: range1919752
display id: range1919752
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7865
end: 7870
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation1919754
display id: annotation1919754
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919754/range1919754/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919754/range1919754/1
type: Range
persistent identity: range1919754
display id: range1919754
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7922
end: 7926
orientation: inline

TliI
type: SequenceAnnotation
persistent identity: annotation1919753
display id: annotation1919753
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919753/range1919753/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919753/range1919753/1
type: Range
persistent identity: range1919753
display id: range1919753
version: 1
top level: BBa_M1307
direction: direction/forward
start: 7922
end: 7926
orientation: inline

Pst1
type: SequenceAnnotation
persistent identity: annotation1919755
display id: annotation1919755
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919755/range1919755/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919755/range1919755/1
type: Range
persistent identity: range1919755
display id: range1919755
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8079
end: 8083
orientation: inline

promoter-g2
type: SequenceAnnotation
persistent identity: annotation1919756
display id: annotation1919756
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919756/range1919756/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M1307/annotation1919756/range1919756/1
type: Range
persistent identity: range1919756
display id: range1919756
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8188
end: 8235
orientation: inline

SwaI
type: SequenceAnnotation
persistent identity: annotation1919757
display id: annotation1919757
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919757/range1919757/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919757/range1919757/1
type: Range
persistent identity: range1919757
display id: range1919757
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8202
end: 8209
orientation: inline

rbs-g2
type: SequenceAnnotation
persistent identity: annotation1919758
display id: annotation1919758
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919758/range1919758/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919758/range1919758/1
type: Range
persistent identity: range1919758
display id: range1919758
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8252
end: 8267
orientation: inline

rbs-g1
type: SequenceAnnotation
persistent identity: annotation1919759
display id: annotation1919759
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919759/range1919759/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M1307/annotation1919759/range1919759/1
type: Range
persistent identity: range1919759
display id: range1919759
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8252
end: 8267
orientation: inline

AccI
type: SequenceAnnotation
persistent identity: annotation1919760
display id: annotation1919760
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919760/range1919760/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M1307/annotation1919760/range1919760/1
type: Range
persistent identity: range1919760
display id: range1919760
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8353
end: 8358
orientation: inline

HpaI
type: SequenceAnnotation
persistent identity: annotation1919761
display id: annotation1919761
version: 1
top level: BBa_M1307
location: https://synbiohub.org/public/igem/BBa_M1307/annotation1919761/range1919761/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M1307/annotation1919761/range1919761/1
type: Range
persistent identity: range1919761
display id: range1919761
version: 1
top level: BBa_M1307
direction: direction/forward
start: 8669
end: 8669
orientation: inline

https://synbiohub.org/public/igem/BBa_M1307_sequence/1
type: Sequence
persistent identity: BBa M1307 sequence
display id: BBa_M1307_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatcc…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z