From c. Acetalobutylicum genome, optimized for Synechococcus PCC7002
Complete pathway for 1-butanol production, optimized for Synechococcus PCC7002
Double terminator consisting of BBa_B0012 and BBa_B0011
bcd part comes from a genomic sequence and the promoter and ribosome binding site are already a part on their own (BBa_K390018)
bcd gene with promoter and ribosome binding site
Expresses the translation of a protein whose main function is to transfer electrons from crotonyl-CoA to butyryl-CoA, using NADH as the oxidizing agent. This gene was extracted from Megasphaera elsdenii, but optimized for Clostridium synechococcus using DNA 2.0 software. A promoter sequence was also added as a recognition site for its integration into the Clostridium synechococcus genome.
Optimized for use in Clostridium synechococcus. Promoter sequence added for its integration into the Clostridium synechococcus genome.
Composite of Promoter/RBS and coding sequence
This is more of an intermediate part from synechococcus and mycobacterium.
This is the synechococcus optimized adhE2 gene attached to a synechococcus promoter.
Had to make sure it coded without restriction enzyme sites.
Composite of CRT with ribosome binding site/promoter
This part is an optimized composite of the CRT gene with a promoter and ribosome binding site for Synechococcus sp. PCC 7002.
hbd gene with sigA promoter/consensus RBS
From C. Acetobutylicum, optimized for Synechococcus PCC 7002
hbd gene with sigA promoter and consensus RBS
Thiolase gene from C. Acetilobutylicum genome, optimized for Synechococcus PCC 7002
Thiolase gene coding region with sigA promoter and consensus RBS for Synechocystis PCC6803
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
From Clostridium Acetobutylicum ATCC 824.
bcd enzyme open reading frame coding sequence.
etfAB gene optimized for Clostridium synechococcus
This gene was collected from Megasphaera elsdenii, but optimized for Clostridium synechococcus using DNA 2.0 software.
Optimized for Clostridium synechococcus.
Clostridium acetobutylicum EA 2018 genomic sequence
Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018
Largest open reading frame from the complete gene.
From the genome of Clostridium Acetobutylicum.
Optimized for Cyanobacterium Synechococcus PCC 7002
This gene was isolated from the Mycobacterium tuberculosis complete genome.
This is a gene used by Clostridium Acetobutylicum in the production of 1-butanol. The coding region codes for an alcohol/aldehyde dehydrogenase.
This sequence was optimized for Synechococcus sp. pcc 7002.
Region coding for thiolase step of 1-butanol synthesis pathway.
Conversion of the iGEM parts registry to SBOL2.1