BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_M1366
1
BBa_M1366
Gliadin (triticum aestivum)
2012-04-10T11:00:00Z
2015-05-08T01:13:56Z
This sequence was backtranslated, optimized for E. coli and modified to remove illegal restriction sites from an aa sequence from triticum aestivum. it endodes the alpha subunit of gliadin.
This sequence codes for gliadin, alpha subunit. It does not include any post-translational action.
false
false
_768_
0
10129
9
Not in stock
false
Some modifications were made to eliminate illegal restriction sites.
false
Eric B Anderson
annotation2172112
1
Coding Sequence
range2172112
1
1
889
annotation2172110
1
Start
range2172110
1
1
3
annotation2172111
1
Stop
range2172111
1
889
891
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_M1365
1
BBa_M1365
Alpha Gliadin Complete
2012-04-23T11:00:00Z
2015-05-08T01:13:56Z
See info of basic part (BBa_M1366)
Alpha Gliadin conding sequence with constitutive promoter and RBS, plus double terminator
false
false
_768_
0
10129
9
Not in stock
false
Added Constitutive promoter & RBS and double terminator
false
Eric B Anderson
component2173204
1
BBa_M1366
component2173205
1
BBa_B0010
component2173199
1
BBa_B0030
component2173197
1
BBa_J23100
component2173207
1
BBa_B0012
annotation2173197
1
BBa_J23100
range2173197
1
1
35
annotation2173199
1
BBa_B0030
range2173199
1
44
58
annotation2173207
1
BBa_B0012
range2173207
1
1052
1092
annotation2173204
1
BBa_M1366
range2173204
1
65
955
annotation2173205
1
BBa_B0010
range2173205
1
964
1043
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_M1366_sequence
1
atgaaaacgttcctgatcctggcactgttagctatcgttgcaacaacagctacaaccgcagtacgtgttccggtaccccaacctcaaccgcaaaatccgagtcaaccgcagccccagcgccaggttccgctcgtccaacagcaacaattccctggccagcagcagcagtttcccccgcagcagccttatccccagccccaaccttttccgagtcagcagccgtacctgcaattacagccgttcccacagccgcagccattccccccgcagttgccgtacccacaaccgccgcctttttcaccccagcaaccatacccgcagcctcaacctcaatacccccagccgcagcaacccattagtcaacagcaggcccaacaacagcagcaacagcagcagcaacaacaacaacagcagcaacagcagcagatcctgccccaaatcttacagcagcaactgataccatgtagagatgtcgtgttacagcagcacaacatcgcccatgccagatcacaggtcttacagcaatcaacctatcagccattacaacagctttgttgccagcagctttggcaaatccctgaacaatcacgctgccaggccatccataatgtagtgcacgccataatcctgcaccagcaacaacagcaacagcaaccgagcagccaggttagtttgcagcagccgcagcagcagtatccctcaggccaaggttttttccagcctagtcaacagaacccgcaggctcagggttcagtacagccacagcaactgccgcagttcgaggaaatccgtaacctggctttacagaccctgccgagaatgtgcaatgtgtatattcccccgtactgctcgacgacgaccgcaccctttggaatttttgggacaaattga
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_M1365_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaaaacgttcctgatcctggcactgttagctatcgttgcaacaacagctacaaccgcagtacgtgttccggtaccccaacctcaaccgcaaaatccgagtcaaccgcagccccagcgccaggttccgctcgtccaacagcaacaattccctggccagcagcagcagtttcccccgcagcagccttatccccagccccaaccttttccgagtcagcagccgtacctgcaattacagccgttcccacagccgcagccattccccccgcagttgccgtacccacaaccgccgcctttttcaccccagcaaccatacccgcagcctcaacctcaatacccccagccgcagcaacccattagtcaacagcaggcccaacaacagcagcaacagcagcagcaacaacaacaacagcagcaacagcagcagatcctgccccaaatcttacagcagcaactgataccatgtagagatgtcgtgttacagcagcacaacatcgcccatgccagatcacaggtcttacagcaatcaacctatcagccattacaacagctttgttgccagcagctttggcaaatccctgaacaatcacgctgccaggccatccataatgtagtgcacgccataatcctgcaccagcaacaacagcaacagcaaccgagcagccaggttagtttgcagcagccgcagcagcagtatccctcaggccaaggttttttccagcctagtcaacagaacccgcaggctcagggttcagtacagccacagcaactgccgcagttcgaggaaatccgtaacctggctttacagaccctgccgagaatgtgcaatgtgtatattcccccgtactgctcgacgacgaccgcaccctttggaatttttgggacaaattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z