BBa_B0040 1 spacer Spacer.1 (generic) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Randomly generated and optimized for several parameters (see Design notes). Released HQ 2013 Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.</P> false false _1_ 0 24 7 In stock false <P> <P><p>The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the <a href="http://www.lifesci.ucsb.edu/~maduro/random.htm">Random DNA Generator </a>engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other <em>E.coli</em> sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. </p> <p> DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur):<br> T->A @ 85<br> T->A @ 42<br> C->T @ 79<br> A->T @ 64<br> A->T @ 31<br> T->A @ 34<br> C->A @ 37<br> Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences.<br> </p> <P>In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. </p><P> The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid. true Vinay S. Mahajan, Brian Chow, Peter Carr annotation1721 1 Spacer-1 range1721 1 1 70 annotation7030 1 BBa_B0040 range7030 1 1 70 BBa_M1366 1 BBa_M1366 Gliadin (triticum aestivum) 2012-04-10T11:00:00Z 2015-05-08T01:13:56Z This sequence was backtranslated, optimized for E. coli and modified to remove illegal restriction sites from an aa sequence from triticum aestivum. it endodes the alpha subunit of gliadin. This sequence codes for gliadin, alpha subunit. It does not include any post-translational action. false false _768_ 0 10129 9 Not in stock false Some modifications were made to eliminate illegal restriction sites. false Eric B Anderson annotation2172111 1 Stop range2172111 1 889 891 annotation2172112 1 Coding Sequence range2172112 1 1 889 annotation2172110 1 Start range2172110 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_J61122 1 BBa_J61122 Ribosome Binding Site Family Member 2007-04-24T11:00:00Z 2015-08-31T01:59:49Z N/A {{JCA_Arkin_RBSFamily}} false false _95_ 0 483 95 It's complicated false N/A false John Anderson BBa_K208003 1 BBa_K208003 OmpA Signal Peptide - Silver Fusion Compatible 2009-10-11T11:00:00Z 2015-05-08T01:11:24Z Anneal OmpA false false _310_ 0 3473 9 It's complicated false Silver Fusion false USU iGEM 2009 annotation2034605 1 Start Codon range2034605 1 1 3 annotation2034604 1 OmpA range2034604 1 1 63 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M1367 1 BBa_M1367 Alpha Gliadin + OmpA signal 2012-04-10T11:00:00Z 2015-05-08T01:13:56Z Drawn on the aa sequence for the protein and the work of Utah State 2009 iGEM team. This construct is designed to produce the alpha subunit of gliadin and modify it for secretion from the cell. false false _768_ 0 10129 9 Not in stock false Fusion protein setup is used. false Eric B Anderson component2172114 1 BBa_J61122 component2172126 1 BBa_B0012 component2172113 1 BBa_J23100 component2172119 1 BBa_K208003 component2172123 1 BBa_M1366 component2172116 1 BBa_B0040 component2172124 1 BBa_B0010 annotation2172124 1 BBa_B0010 range2172124 1 1108 1187 annotation2172123 1 BBa_M1366 range2172123 1 209 1099 annotation2172113 1 BBa_J23100 range2172113 1 1 35 annotation2172119 1 BBa_K208003 range2172119 1 140 202 annotation2172126 1 BBa_B0012 range2172126 1 1196 1236 annotation2172116 1 BBa_B0040 range2172116 1 64 133 annotation2172114 1 BBa_J61122 range2172114 1 44 55 BBa_M1367_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagagaggagctactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctactagatgaaaacgttcctgatcctggcactgttagctatcgttgcaacaacagctacaaccgcagtacgtgttccggtaccccaacctcaaccgcaaaatccgagtcaaccgcagccccagcgccaggttccgctcgtccaacagcaacaattccctggccagcagcagcagtttcccccgcagcagccttatccccagccccaaccttttccgagtcagcagccgtacctgcaattacagccgttcccacagccgcagccattccccccgcagttgccgtacccacaaccgccgcctttttcaccccagcaaccatacccgcagcctcaacctcaatacccccagccgcagcaacccattagtcaacagcaggcccaacaacagcagcaacagcagcagcaacaacaacaacagcagcaacagcagcagatcctgccccaaatcttacagcagcaactgataccatgtagagatgtcgtgttacagcagcacaacatcgcccatgccagatcacaggtcttacagcaatcaacctatcagccattacaacagctttgttgccagcagctttggcaaatccctgaacaatcacgctgccaggccatccataatgtagtgcacgccataatcctgcaccagcaacaacagcaacagcaaccgagcagccaggttagtttgcagcagccgcagcagcagtatccctcaggccaaggttttttccagcctagtcaacagaacccgcaggctcagggttcagtacagccacagcaactgccgcagttcgaggaaatccgtaacctggctttacagaccctgccgagaatgtgcaatgtgtatattcccccgtactgctcgacgacgaccgcaccctttggaatttttgggacaaattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0040_sequence 1 aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca BBa_M1366_sequence 1 atgaaaacgttcctgatcctggcactgttagctatcgttgcaacaacagctacaaccgcagtacgtgttccggtaccccaacctcaaccgcaaaatccgagtcaaccgcagccccagcgccaggttccgctcgtccaacagcaacaattccctggccagcagcagcagtttcccccgcagcagccttatccccagccccaaccttttccgagtcagcagccgtacctgcaattacagccgttcccacagccgcagccattccccccgcagttgccgtacccacaaccgccgcctttttcaccccagcaaccatacccgcagcctcaacctcaatacccccagccgcagcaacccattagtcaacagcaggcccaacaacagcagcaacagcagcagcaacaacaacaacagcagcaacagcagcagatcctgccccaaatcttacagcagcaactgataccatgtagagatgtcgtgttacagcagcacaacatcgcccatgccagatcacaggtcttacagcaatcaacctatcagccattacaacagctttgttgccagcagctttggcaaatccctgaacaatcacgctgccaggccatccataatgtagtgcacgccataatcctgcaccagcaacaacagcaacagcaaccgagcagccaggttagtttgcagcagccgcagcagcagtatccctcaggccaaggttttttccagcctagtcaacagaacccgcaggctcagggttcagtacagccacagcaactgccgcagttcgaggaaatccgtaacctggctttacagaccctgccgagaatgtgcaatgtgtatattcccccgtactgctcgacgacgaccgcaccctttggaatttttgggacaaattga BBa_K208003_sequence 1 atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcc BBa_J61122_sequence 1 aaagagaggagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z