BBa_M1405 1 BBa_M1405 ribA 2011-04-22T11:00:00Z 2015-05-08T01:13:56Z Escherichia coli. rib A gene, first gene used in the production of vitamin B2. Converts GTP to 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one. E.coli ribA gene for GTP cyclohydrolase II. false false _768_ 0 8954 9 Not in stock false It has an illegal PstI site between 1087 to 1093, which has been mutated out. false Asif Rahman annotation2117032 1 start range2117032 1 6 8 annotation2117033 1 stop range2117033 1 1471 1473 annotation2117031 1 cds range2117031 1 1 1535 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_M1409 1 BBa_M1409 ribD 2011-04-23T11:00:00Z 2015-05-08T01:13:56Z E.coli ribD gene for synthesizing riboflavin. false false _768_ 0 8958 9 Not in stock false have to remove one PstI site from the gene false Jia Zeng annotation2117076 1 start range2117076 1 1 3 annotation2117077 1 stop range2117077 1 1102 1104 annotation2117078 1 cds range2117078 1 1 1104 BBa_M1441 1 BBa_M1441 cof thiamin pyrimidine pyrophosphate hydrolase 2011-04-22T11:00:00Z 2015-05-08T01:13:56Z Escherichia coli str. K-12 substr. MG1655 Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF. Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada. Dephosphorylation of phosphoric acid ester. Shows high catalytic efficiency and affinity to a wide range of phosphorylated metabolites that are intermediates of various metabolic reactions. false false _768_ 0 8967 9 Not in stock false None false Candace Clark annotation2117040 1 Coding range2117040 1 1 819 annotation2117041 1 Stop range2117041 1 817 819 annotation2117039 1 Start range2117039 1 1 3 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_M1406 1 BBa_M1406 Promoter + RBS 2011-04-22T11:00:00Z 2015-05-08T01:13:56Z Registry Promoter (J23119) + RBS (B0030) composite false false _768_ 0 8954 9 Not in stock false None false Asif Rahman component2117037 1 BBa_B0030 component2117035 1 BBa_J23119 annotation2117035 1 BBa_J23119 range2117035 1 1 35 annotation2117037 1 BBa_B0030 range2117037 1 44 58 BBa_M1407 1 BBa_M1407 ribA ribD and PP 2011-04-23T11:00:00Z 2015-05-08T01:13:56Z E.coli ribA ribD and PP genes used for riboflavin synthesis false false _768_ 0 8954 9 Not in stock false none false Asif Rahman component2117100 1 BBa_B0030 component2117105 1 BBa_M1441 component2117098 1 BBa_M1409 component2117087 1 BBa_M1406 component2117093 1 BBa_B0030 component2117112 1 BBa_B0015 component2117091 1 BBa_M1405 annotation2117105 1 BBa_M1441 range2117105 1 2766 3584 annotation2117093 1 BBa_B0030 range2117093 1 1610 1624 annotation2117112 1 BBa_B0015 range2117112 1 3593 3721 annotation2117100 1 BBa_B0030 range2117100 1 2745 2759 annotation2117087 1 BBa_M1406 range2117087 1 1 58 annotation2117091 1 BBa_M1405 range2117091 1 67 1601 annotation2117098 1 BBa_M1409 range2117098 1 1633 2736 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M1409_sequence 1 gtgcaggacgagtattacatggcgcgggcgctaaagctggcgcaacgaggacgttttaccacgcatcccaacccgaatgtcgggtgcgtcattgtcaaagatggcgaaattgtcggtgaaggttaccaccaacgtgcgggtgaaccacatgccgaagtacacgcgttgcgtatggcgggtgaaaaagccaaaggtgcgaccgcctatgtcacactcgaaccctgtagccatcatggtcgtacgccaccgtgctgtgacgcactcatcgccgctggcgtagcgcgcgtggttgcctcgatgcaagatcctaacccgcaggtcgctgggcgtggactttaccgtctgcaacaggctggcattgacgtcagccacggcctgatgatgagtgaagccgagcaattgaataaaggctttctcaagcggatgcgcaccggctttccttatattcagttaaaacttggcgcatcgcttgatggtcgcacggcgatggcgagcggcgaaagccagtggatcacttcgccccaggcgcggcgtgatgtacaactactgcgcgcgcaaagtcatgccattttaaccagcagcgccacggtgctggcggatgatcctgccttaacggtgcgttggtctgaactggatgaacaaactcaggcgctctatccgcaacaaaatctccgtcagccgatacgtattgtgattgatagccaaaatcgcgtgacgccggtacatcgcattgtgcagcagcccggcgaaacctggttcgcgcgtacgcaggaagattctcgtgagtggccggaaacggtgcgtaccttgctgattccagagcataaaggtcatctggatctggttgtactgatgatgcaactgggtaaacagcaaattaacagcatctgggtggaagcggggccaacgctcgctggcgcattgctgcaagcgggtttagtcgatgagctgattgtctatatcgcacctaaactattaggcagcgacgcccgcggattatgcacgctgccagggcttgagaaattagccgacgccccccaatttaaattcaaagagatacgtcatgtaggcccggatgtttgcctgcatttagtgggtgcatga BBa_M1441_sequence 1 atggctcgtctggcagcatttgatatggatggcactttattgatgcccgaccatcatttaggtgagaaaaccctctctactttggcgcgactgcgtgaacgcgacattaccctcacttttgccacggggcgtcatgcgctggagatgcagcatattctcggggcgctatcgctggatgcgtatttgattaccggcaacggaacgcgcgtgcattctctggaaggtgaacttttacatcgtgatgatttacctgcggatgtcgcggagctggtgctgtatcagcaatgggatacccgagccagcatgcatatcttcaatgacgacggttggtttaccgggaaagagatccctgcgttgttgcaggcatttgtctatagcggttttcgttatcagataatcgatgtcaaaaaaatgccactcggcagcgtcaccaagatctgcttctgtggcgatcacgacgatcttacacgcttgcagatccagctatacgaagcattaggcgagcgtgcacatttgtgtttttccgccacggattgcctcgaagtgctgccggtgggctgcaataaaggcgctgcattgacggtgctgacccaacatttaggtttatcgttgcgcgattgcatggcctttggtgatgcgatgaacgatcgcgaaatgttagtcagcgtcggtagcggatttattatgggcaatgcgatgccgcaactgcgcgcggagctcccgcatttaccggtgattggacattgccgaaatcaggctgtctctcactatttgacgcactggctggactatccacatctaccttattcccccgaataa BBa_M1405_sequence 1 ttcccatgactcccgttgcccagaccagcaagatagcaatggttaacgttcgccgacgcggccacagcaaaccaacggccagcagtgcccaactggcagcaaacatcgtgtgaccggaaggaaaggcaaaccccgtctctttctgccagtgtgaacgcaaatattgtgggatatttttctcttcagccaactgttctttcactagatttccgcgttctgctcgctttaaagtgtagaactcatcaaccggaatatgatgtgttttttccagccagataacaaaaggtcgtggttcctggactttgtctttgatccaggatttaacgccttgtcccacaaggattgcggccgccagaatggcaaataatacaaaggcagccttaatgcgaaaacgcagacaccagagaaaccagccgaataaaatcaaatgtgtaatgacgccccagggctgggtgacagtttcagtaacccaaaaagccgcttttagtagccaactttgttctccaggttgccaacgccagccagaaatccatacggctactggcatgacaagcaatagtgcagctcccactgcggtacgtctggcaatcgaacgcatggcctctcctttttgataagtcccacaatcataactgaaaacgccagttccaggaaaaattgacagatttgtgccattccgtgaacgatcgacgcgtcgtgattaggtgaaccccttctcgttatggcaaaataagccaatacagaaccagcattatctggagaatttcatgcagcttaaacgtgtggcagaagccaaactgccaaccccatggggcgatttcctgatggtgggatttgaagaactggcaaccggacacgatcatgtcgcgctagtctatggcgatatttccgggcataccccggtacttgcgcgcgtccattccgaatgtctgaccggtgacgccctgttcagcttgcgctgcgattgtggcttccagctcgaagcggcattgacgcaaattgccgaggaaggccgtggtattttgctgtatcaccgtcaggaaggtcgtaacattggtctgctgaataaaatccgcgcttacgcactgtaggatcaaggttacgataccgtagaggctaaccaccagttaggcttcgccgctgatgagcgcgacttcactctttgcgctgatatgttcaaactccttggcgtcaatgaagtccgcttgttaaccaataacccgaaaaaagtcgaaattctgaccgaagcagggattaatattgttgaacgcgtaccattgattgtaggtcgtaaccccaataacgaacattatctcgataccaaagccgagaaaatgggccatttgctgaacaaataaccctcttgcattgtgtaattcatttgcttgccggaagcaaaataaccggcaagcaaatagttgttacttcaacatattacgaatgacataatgcaaaatgccgtcgttctggtagtaggtcaactccgtcgcggtgtcgatacgacaacggcagggtacgacttcctggctaccatc BBa_B0030_sequence 1 attaaagaggagaaa BBa_M1406_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaa BBa_M1407_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagttcccatgactcccgttgcccagaccagcaagatagcaatggttaacgttcgccgacgcggccacagcaaaccaacggccagcagtgcccaactggcagcaaacatcgtgtgaccggaaggaaaggcaaaccccgtctctttctgccagtgtgaacgcaaatattgtgggatatttttctcttcagccaactgttctttcactagatttccgcgttctgctcgctttaaagtgtagaactcatcaaccggaatatgatgtgttttttccagccagataacaaaaggtcgtggttcctggactttgtctttgatccaggatttaacgccttgtcccacaaggattgcggccgccagaatggcaaataatacaaaggcagccttaatgcgaaaacgcagacaccagagaaaccagccgaataaaatcaaatgtgtaatgacgccccagggctgggtgacagtttcagtaacccaaaaagccgcttttagtagccaactttgttctccaggttgccaacgccagccagaaatccatacggctactggcatgacaagcaatagtgcagctcccactgcggtacgtctggcaatcgaacgcatggcctctcctttttgataagtcccacaatcataactgaaaacgccagttccaggaaaaattgacagatttgtgccattccgtgaacgatcgacgcgtcgtgattaggtgaaccccttctcgttatggcaaaataagccaatacagaaccagcattatctggagaatttcatgcagcttaaacgtgtggcagaagccaaactgccaaccccatggggcgatttcctgatggtgggatttgaagaactggcaaccggacacgatcatgtcgcgctagtctatggcgatatttccgggcataccccggtacttgcgcgcgtccattccgaatgtctgaccggtgacgccctgttcagcttgcgctgcgattgtggcttccagctcgaagcggcattgacgcaaattgccgaggaaggccgtggtattttgctgtatcaccgtcaggaaggtcgtaacattggtctgctgaataaaatccgcgcttacgcactgtaggatcaaggttacgataccgtagaggctaaccaccagttaggcttcgccgctgatgagcgcgacttcactctttgcgctgatatgttcaaactccttggcgtcaatgaagtccgcttgttaaccaataacccgaaaaaagtcgaaattctgaccgaagcagggattaatattgttgaacgcgtaccattgattgtaggtcgtaaccccaataacgaacattatctcgataccaaagccgagaaaatgggccatttgctgaacaaataaccctcttgcattgtgtaattcatttgcttgccggaagcaaaataaccggcaagcaaatagttgttacttcaacatattacgaatgacataatgcaaaatgccgtcgttctggtagtaggtcaactccgtcgcggtgtcgatacgacaacggcagggtacgacttcctggctaccatctactagagattaaagaggagaaatactagaggtgcaggacgagtattacatggcgcgggcgctaaagctggcgcaacgaggacgttttaccacgcatcccaacccgaatgtcgggtgcgtcattgtcaaagatggcgaaattgtcggtgaaggttaccaccaacgtgcgggtgaaccacatgccgaagtacacgcgttgcgtatggcgggtgaaaaagccaaaggtgcgaccgcctatgtcacactcgaaccctgtagccatcatggtcgtacgccaccgtgctgtgacgcactcatcgccgctggcgtagcgcgcgtggttgcctcgatgcaagatcctaacccgcaggtcgctgggcgtggactttaccgtctgcaacaggctggcattgacgtcagccacggcctgatgatgagtgaagccgagcaattgaataaaggctttctcaagcggatgcgcaccggctttccttatattcagttaaaacttggcgcatcgcttgatggtcgcacggcgatggcgagcggcgaaagccagtggatcacttcgccccaggcgcggcgtgatgtacaactactgcgcgcgcaaagtcatgccattttaaccagcagcgccacggtgctggcggatgatcctgccttaacggtgcgttggtctgaactggatgaacaaactcaggcgctctatccgcaacaaaatctccgtcagccgatacgtattgtgattgatagccaaaatcgcgtgacgccggtacatcgcattgtgcagcagcccggcgaaacctggttcgcgcgtacgcaggaagattctcgtgagtggccggaaacggtgcgtaccttgctgattccagagcataaaggtcatctggatctggttgtactgatgatgcaactgggtaaacagcaaattaacagcatctgggtggaagcggggccaacgctcgctggcgcattgctgcaagcgggtttagtcgatgagctgattgtctatatcgcacctaaactattaggcagcgacgcccgcggattatgcacgctgccagggcttgagaaattagccgacgccccccaatttaaattcaaagagatacgtcatgtaggcccggatgtttgcctgcatttagtgggtgcatgatactagagattaaagaggagaaatactagatggctcgtctggcagcatttgatatggatggcactttattgatgcccgaccatcatttaggtgagaaaaccctctctactttggcgcgactgcgtgaacgcgacattaccctcacttttgccacggggcgtcatgcgctggagatgcagcatattctcggggcgctatcgctggatgcgtatttgattaccggcaacggaacgcgcgtgcattctctggaaggtgaacttttacatcgtgatgatttacctgcggatgtcgcggagctggtgctgtatcagcaatgggatacccgagccagcatgcatatcttcaatgacgacggttggtttaccgggaaagagatccctgcgttgttgcaggcatttgtctatagcggttttcgttatcagataatcgatgtcaaaaaaatgccactcggcagcgtcaccaagatctgcttctgtggcgatcacgacgatcttacacgcttgcagatccagctatacgaagcattaggcgagcgtgcacatttgtgtttttccgccacggattgcctcgaagtgctgccggtgggctgcaataaaggcgctgcattgacggtgctgacccaacatttaggtttatcgttgcgcgattgcatggcctttggtgatgcgatgaacgatcgcgaaatgttagtcagcgtcggtagcggatttattatgggcaatgcgatgccgcaactgcgcgcggagctcccgcatttaccggtgattggacattgccgaaatcaggctgtctctcactatttgacgcactggctggactatccacatctaccttattcccccgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z