##gff-version 3
##sequence-region BBa_M1407 1 3721
BBa_M1407	.	engineered_region	1	58	.	+	0	ID=BBa_M1406;Name=BBa_M1406
BBa_M1407	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_M1406
BBa_M1407	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1407	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1407	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1407	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_M1406
BBa_M1407	.	CDS	2766	3584	.	+	0	ID=BBa_M1441;Name=BBa_M1441
BBa_M1407	.	start_codon	2766	2768	.	+	0	ID=annotation2117039;Name=Start;Parent=BBa_M1441
BBa_M1407	.	stop_codon	3582	3584	.	+	0	ID=annotation2117041;Name=Stop;Parent=BBa_M1441
BBa_M1407	.	CDS	2766	3584	.	+	0	ID=annotation2117040;Name=Coding;Parent=BBa_M1441
BBa_M1407	.	CDS	67	1601	.	+	0	ID=BBa_M1405;Name=BBa_M1405
BBa_M1407	.	start_codon	72	74	.	+	0	ID=annotation2117032;Name=start;Parent=BBa_M1405
BBa_M1407	.	stop_codon	1537	1539	.	+	0	ID=annotation2117033;Name=stop;Parent=BBa_M1405
BBa_M1407	.	CDS	67	1601	.	+	0	ID=annotation2117031;Name=cds;Parent=BBa_M1405
BBa_M1407	.	ribosome_entry_site	2745	2759	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1407	.	ribosome_entry_site	2752	2756	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1407	.	ribosome_entry_site	2745	2759	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1407	.	engineered_region	2745	2759	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1407	.	CDS	1633	2736	.	+	0	ID=BBa_M1409;Name=BBa_M1409
BBa_M1407	.	stop_codon	2734	2736	.	+	0	ID=annotation2117077;Name=stop;Parent=BBa_M1409
BBa_M1407	.	start_codon	1633	1635	.	+	0	ID=annotation2117076;Name=start;Parent=BBa_M1409
BBa_M1407	.	CDS	1633	2736	.	+	0	ID=annotation2117078;Name=cds;Parent=BBa_M1409
BBa_M1407	.	ribosome_entry_site	1610	1624	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1407	.	ribosome_entry_site	1617	1621	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1407	.	ribosome_entry_site	1610	1624	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1407	.	engineered_region	1610	1624	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1407	.	terminator	3593	3721	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1407	.	terminator	3681	3721	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1407	.	polyA_site	3708	3721	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1407	.	stem_loop	3688	3707	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1407	.	engineered_region	3681	3721	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1407	.	stop_codon	3714	3714	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1407	.	terminator	3593	3672	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1407	.	stem_loop	3604	3647	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1407	.	engineered_region	3593	3672	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_M1407
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagttcccatgactcc
cgttgcccagaccagcaagatagcaatggttaacgttcgccgacgcggccacagcaaaccaacggccagcagtgcccaa
ctggcagcaaacatcgtgtgaccggaaggaaaggcaaaccccgtctctttctgccagtgtgaacgcaaatattgtggga
tatttttctcttcagccaactgttctttcactagatttccgcgttctgctcgctttaaagtgtagaactcatcaaccgg
aatatgatgtgttttttccagccagataacaaaaggtcgtggttcctggactttgtctttgatccaggatttaacgcct
tgtcccacaaggattgcggccgccagaatggcaaataatacaaaggcagccttaatgcgaaaacgcagacaccagagaa
accagccgaataaaatcaaatgtgtaatgacgccccagggctgggtgacagtttcagtaacccaaaaagccgcttttag
tagccaactttgttctccaggttgccaacgccagccagaaatccatacggctactggcatgacaagcaatagtgcagct
cccactgcggtacgtctggcaatcgaacgcatggcctctcctttttgataagtcccacaatcataactgaaaacgccag
ttccaggaaaaattgacagatttgtgccattccgtgaacgatcgacgcgtcgtgattaggtgaaccccttctcgttatg
gcaaaataagccaatacagaaccagcattatctggagaatttcatgcagcttaaacgtgtggcagaagccaaactgcca
accccatggggcgatttcctgatggtgggatttgaagaactggcaaccggacacgatcatgtcgcgctagtctatggcg
atatttccgggcataccccggtacttgcgcgcgtccattccgaatgtctgaccggtgacgccctgttcagcttgcgctg
cgattgtggcttccagctcgaagcggcattgacgcaaattgccgaggaaggccgtggtattttgctgtatcaccgtcag
gaaggtcgtaacattggtctgctgaataaaatccgcgcttacgcactgtaggatcaaggttacgataccgtagaggcta
accaccagttaggcttcgccgctgatgagcgcgacttcactctttgcgctgatatgttcaaactccttggcgtcaatga
agtccgcttgttaaccaataacccgaaaaaagtcgaaattctgaccgaagcagggattaatattgttgaacgcgtacca
ttgattgtaggtcgtaaccccaataacgaacattatctcgataccaaagccgagaaaatgggccatttgctgaacaaat
aaccctcttgcattgtgtaattcatttgcttgccggaagcaaaataaccggcaagcaaatagttgttacttcaacatat
tacgaatgacataatgcaaaatgccgtcgttctggtagtaggtcaactccgtcgcggtgtcgatacgacaacggcaggg
tacgacttcctggctaccatctactagagattaaagaggagaaatactagaggtgcaggacgagtattacatggcgcgg
gcgctaaagctggcgcaacgaggacgttttaccacgcatcccaacccgaatgtcgggtgcgtcattgtcaaagatggcg
aaattgtcggtgaaggttaccaccaacgtgcgggtgaaccacatgccgaagtacacgcgttgcgtatggcgggtgaaaa
agccaaaggtgcgaccgcctatgtcacactcgaaccctgtagccatcatggtcgtacgccaccgtgctgtgacgcactc
atcgccgctggcgtagcgcgcgtggttgcctcgatgcaagatcctaacccgcaggtcgctgggcgtggactttaccgtc
tgcaacaggctggcattgacgtcagccacggcctgatgatgagtgaagccgagcaattgaataaaggctttctcaagcg
gatgcgcaccggctttccttatattcagttaaaacttggcgcatcgcttgatggtcgcacggcgatggcgagcggcgaa
agccagtggatcacttcgccccaggcgcggcgtgatgtacaactactgcgcgcgcaaagtcatgccattttaaccagca
gcgccacggtgctggcggatgatcctgccttaacggtgcgttggtctgaactggatgaacaaactcaggcgctctatcc
gcaacaaaatctccgtcagccgatacgtattgtgattgatagccaaaatcgcgtgacgccggtacatcgcattgtgcag
cagcccggcgaaacctggttcgcgcgtacgcaggaagattctcgtgagtggccggaaacggtgcgtaccttgctgattc
cagagcataaaggtcatctggatctggttgtactgatgatgcaactgggtaaacagcaaattaacagcatctgggtgga
agcggggccaacgctcgctggcgcattgctgcaagcgggtttagtcgatgagctgattgtctatatcgcacctaaacta
ttaggcagcgacgcccgcggattatgcacgctgccagggcttgagaaattagccgacgccccccaatttaaattcaaag
agatacgtcatgtaggcccggatgtttgcctgcatttagtgggtgcatgatactagagattaaagaggagaaatactag
atggctcgtctggcagcatttgatatggatggcactttattgatgcccgaccatcatttaggtgagaaaaccctctcta
ctttggcgcgactgcgtgaacgcgacattaccctcacttttgccacggggcgtcatgcgctggagatgcagcatattct
cggggcgctatcgctggatgcgtatttgattaccggcaacggaacgcgcgtgcattctctggaaggtgaacttttacat
cgtgatgatttacctgcggatgtcgcggagctggtgctgtatcagcaatgggatacccgagccagcatgcatatcttca
atgacgacggttggtttaccgggaaagagatccctgcgttgttgcaggcatttgtctatagcggttttcgttatcagat
aatcgatgtcaaaaaaatgccactcggcagcgtcaccaagatctgcttctgtggcgatcacgacgatcttacacgcttg
cagatccagctatacgaagcattaggcgagcgtgcacatttgtgtttttccgccacggattgcctcgaagtgctgccgg
tgggctgcaataaaggcgctgcattgacggtgctgacccaacatttaggtttatcgttgcgcgattgcatggcctttgg
tgatgcgatgaacgatcgcgaaatgttagtcagcgtcggtagcggatttattatgggcaatgcgatgccgcaactgcgc
gcggagctcccgcatttaccggtgattggacattgccgaaatcaggctgtctctcactatttgacgcactggctggact
atccacatctaccttattcccccgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata