##gff-version 3
##sequence-region BBa_M1408 1 2030
BBa_M1408	.	ribosome_entry_site	729	743	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1408	.	ribosome_entry_site	736	740	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1408	.	ribosome_entry_site	729	743	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1408	.	engineered_region	729	743	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1408	.	ribosome_entry_site	1229	1243	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1408	.	ribosome_entry_site	1236	1240	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1408	.	ribosome_entry_site	1229	1243	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1408	.	engineered_region	1229	1243	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1408	.	CDS	67	720	.	+	0	ID=BBa_M1440;Name=BBa_M1440
BBa_M1408	.	stop_codon	718	720	.	+	0	ID=annotation2116398;Name=Stop;Parent=BBa_M1440
BBa_M1408	.	CDS	67	720	.	+	0	ID=annotation2116399;Name=Coding Sequence ;Parent=BBa_M1440
BBa_M1408	.	start_codon	67	69	.	+	0	ID=annotation2116397;Name=Start;Parent=BBa_M1440
BBa_M1408	.	engineered_region	1	58	.	+	0	ID=BBa_M1406;Name=BBa_M1406
BBa_M1408	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_M1406
BBa_M1408	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1408	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1408	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1408	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_M1406
BBa_M1408	.	CDS	750	1220	.	+	0	ID=BBa_M1411;Name=BBa_M1411
BBa_M1408	.	start_codon	750	752	.	+	0	ID=annotation2115807;Name=Start;Parent=BBa_M1411
BBa_M1408	.	stop_codon	1218	1220	.	+	0	ID=annotation2115808;Name=Stop;Parent=BBa_M1411
BBa_M1408	.	CDS	750	1220	.	+	0	ID=annotation2115809;Name=Coding;Parent=BBa_M1411
BBa_M1408	.	CDS	1252	1893	.	+	0	ID=BBa_M1410;Name=BBa_M1410
BBa_M1408	.	stop_codon	1891	1893	.	+	0	ID=annotation2115805;Name=Stop;Parent=BBa_M1410
BBa_M1408	.	start_codon	1252	1254	.	+	0	ID=annotation2115804;Name=Start;Parent=BBa_M1410
BBa_M1408	.	CDS	1252	1893	.	+	0	ID=annotation2115806;Name=Coding;Parent=BBa_M1410
BBa_M1408	.	terminator	1902	2030	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1408	.	terminator	1990	2030	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1408	.	polyA_site	2017	2030	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1408	.	stem_loop	1997	2016	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1408	.	engineered_region	1990	2030	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1408	.	stop_codon	2023	2023	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1408	.	terminator	1902	1981	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1408	.	stem_loop	1913	1956	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1408	.	engineered_region	1902	1981	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_M1408
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtcagctggcttta
cgctcatgtgcctgacggtatgccaccaggtcttcaatagtcacgagcgccatattgtgtttattggcaaactcaatac
actctggtgcacgcgccatcgtgccatcgtcattagtcagctcacacagtacaccagccggtttaaagcctgccagcgt
catcagatcaatagttgcttcagtatgaccgccacgcgtcagtacaccacctgcctgagcgcgaagtgggaaaacgtgg
ccaggacgattcagatctgacggttttgcgccatcggcaatcgctgcgcgaacggtcgtaatacggtcagcggcagaaa
caccggtagtcacaccttcagctgcttcaatggtcacggtaaaaccggtgccataggcgctggtgttattttctaccat
cattggcagatcgagttgtttacggcgatcttcagtaatgcacaggcaaacaataccgctaccgtggcgaatggtcagc
gccatctgctcaacagtcatggtttctgccgggaagatcatatcaccttcgttttcacggtcttcatcatcaagcacca
ttacaccgcgtccttcacgcagcgcagccagtgcattttcaacacgttcgaaaggcgtaccaaaagaggaaagtagcgt
ctgattcattactagagattaaagaggagaaatactagatgaacattattgaagctaacgttgctaccccggacgctcg
cgtcgccatcaccatcgcgcgtttcaacaactttatcaatgacagcctgctggaaggtgcaattgacgcactgaaacgt
atcggtcaggtaaaagatgaaaacattaccgttgtttgggtgcctggtgcctatgagctgccgctggcggcgggtgcac
tggctaaaaccggtaaatacgacgcggtgattgcgctgggtacggttattcgtggtggcactgcccactttgaatatgt
cgctggtggtgcaagcaacggcctggcgcatgttgcccaggacagcgaaattccggttgcttttggggttctgaccact
gaaagcattgaacaagcgatcgaacgtgctggcaccaaagctggcaacaaaggtgcagaagctgcactgaccgcgcttg
aaatgattaatgtattgaaagccatcaaggcctgatactagagattaaagaggagaaatactagagtcaggcttctgtg
cctggttgattcatggcattttctcgtgccgccagcacacgttctaccgtatctaccactgcctgagtttgtggatcga
tttcaatgttgacgcgtgcgccaagttttttcttcccaagagtcgtgcgttccagtgtttccggaattaaatggacgca
aaaacgcgttggcgtgacttcgccgacggtcaggctaataccgtcgatgccaataaatcctttgtacagaatatatttc
atcaactgactatcctggactttaaaccagatctggcgattattttctgaggttaatattttcgccacttcagcagtgg
tcataatatgacctgacattaagtgtccgccaatttcatcactgaatttcgccgcacgctcaacgtttacccaatcccc
cacttttaaatcgccaagattggtaatgcgtaacgtttctttcatcaggtcaaaactgacatggttgccgttaatttcc
gtcacggtcaggcagcaaccgttatgcgccacggaagcaccggtttccaggccgtccagcatgtggtcgggtaactcca
ccacatgcgtacgaaaatttggtttctcgtcaatcgacaccagttttgcggtgccctgtacaatccccgtaaacattac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata