BBa_M1408
type: ComponentDefinition
persistent identity: BBa M1408
display id: BBa_M1408
version: 1
was generated by: iGEM to SBOL conversion
description: ribB ribE ribC
created: 2011-04-23T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Ecoli
owned by: james
owned by: myers
mutable description: Composite part of rib B rib E and rib C with promoter, rbs and stop codon
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8954
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Asif Rahman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_M1410
component: BBa_B0030
component: BBa_M1411
component: BBa_M1440
component: BBa_M1406
sequence annotation: BBa_M1406
sequence annotation: BBa_M1440
sequence annotation: BBa_B0030
sequence annotation: BBa_M1411
sequence annotation: BBa_M1410
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1408_sequence/1

BBa_B0015
type: Component
persistent identity: component2117075
display id: component2117075
version: 1
top level: BBa_M1408
definition: BBa_B0015
access: public

BBa_M1410
type: Component
persistent identity: component2117068
display id: component2117068
version: 1
top level: BBa_M1408
definition: BBa_M1410
access: public

BBa_B0030
type: Component
persistent identity: component2117063
display id: component2117063
version: 1
top level: BBa_M1408
definition: BBa_B0030
access: public

BBa_M1411
type: Component
persistent identity: component2117061
display id: component2117061
version: 1
top level: BBa_M1408
definition: BBa_M1411
access: public

BBa_B0030
type: Component
persistent identity: component2117056
display id: component2117056
version: 1
top level: BBa_M1408
definition: BBa_B0030
access: public

BBa_M1440
type: Component
persistent identity: component2117054
display id: component2117054
version: 1
top level: BBa_M1408
definition: BBa_M1440
access: public

BBa_M1406
type: Component
persistent identity: component2117050
display id: component2117050
version: 1
top level: BBa_M1408
definition: BBa_M1406
access: public

BBa_M1406
type: SequenceAnnotation
persistent identity: annotation2117050
display id: annotation2117050
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117050/range2117050/1
component: BBa_M1406

https://synbiohub.org/public/igem/BBa_M1408/annotation2117050/range2117050/1
type: Range
persistent identity: range2117050
display id: range2117050
version: 1
top level: BBa_M1408
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_M1440
type: SequenceAnnotation
persistent identity: annotation2117054
display id: annotation2117054
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117054/range2117054/1
component: BBa_M1440

https://synbiohub.org/public/igem/BBa_M1408/annotation2117054/range2117054/1
type: Range
persistent identity: range2117054
display id: range2117054
version: 1
top level: BBa_M1408
direction: direction/reverse
start: 67
end: 720
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2117056
display id: annotation2117056
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117056/range2117056/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1408/annotation2117056/range2117056/1
type: Range
persistent identity: range2117056
display id: range2117056
version: 1
top level: BBa_M1408
direction: direction/forward
start: 729
end: 743
orientation: inline

BBa_M1411
type: SequenceAnnotation
persistent identity: annotation2117061
display id: annotation2117061
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117061/range2117061/1
component: BBa_M1411

https://synbiohub.org/public/igem/BBa_M1408/annotation2117061/range2117061/1
type: Range
persistent identity: range2117061
display id: range2117061
version: 1
top level: BBa_M1408
direction: direction/reverse
start: 750
end: 1220
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2117063
display id: annotation2117063
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117063/range2117063/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1408/annotation2117063/range2117063/1
type: Range
persistent identity: range2117063
display id: range2117063
version: 1
top level: BBa_M1408
direction: direction/forward
start: 1229
end: 1243
orientation: inline

BBa_M1410
type: SequenceAnnotation
persistent identity: annotation2117068
display id: annotation2117068
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117068/range2117068/1
component: BBa_M1410

https://synbiohub.org/public/igem/BBa_M1408/annotation2117068/range2117068/1
type: Range
persistent identity: range2117068
display id: range2117068
version: 1
top level: BBa_M1408
direction: direction/reverse
start: 1252
end: 1893
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2117075
display id: annotation2117075
version: 1
top level: BBa_M1408
location: https://synbiohub.org/public/igem/BBa_M1408/annotation2117075/range2117075/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1408/annotation2117075/range2117075/1
type: Range
persistent identity: range2117075
display id: range2117075
version: 1
top level: BBa_M1408
direction: direction/reverse
start: 1902
end: 2030
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_M1410
type: ComponentDefinition
persistent identity: BBa M1410
display id: BBa_M1410
version: 1
was generated by: iGEM to SBOL conversion
description: ribC gene
created: 2011-04-08T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Escherichia coli K-12 MG1655 genome, ribC-pykF region.
owned by: james
owned by: myers
mutable description: Codes for the enzyme ribflavin synthase that converts 6,7-dimethyl-8-(1-D-ribityl)lumazine to riboflavin, or vitamin B2.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8963
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Does not have start codon ATG.
star: false
creator: Sarah Prince
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1410_sequence/1

Coding
type: SequenceAnnotation
persistent identity: annotation2115806
display id: annotation2115806
version: 1
top level: BBa_M1410
location: https://synbiohub.org/public/igem/BBa_M1410/annotation2115806/range2115806/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1410/annotation2115806/range2115806/1
type: Range
persistent identity: range2115806
display id: range2115806
version: 1
top level: BBa_M1410
direction: direction/forward
start: 1
end: 642
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2115804
display id: annotation2115804
version: 1
top level: BBa_M1410
location: https://synbiohub.org/public/igem/BBa_M1410/annotation2115804/range2115804/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1410/annotation2115804/range2115804/1
type: Range
persistent identity: range2115804
display id: range2115804
version: 1
top level: BBa_M1410
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2115805
display id: annotation2115805
version: 1
top level: BBa_M1410
location: https://synbiohub.org/public/igem/BBa_M1410/annotation2115805/range2115805/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1410/annotation2115805/range2115805/1
type: Range
persistent identity: range2115805
display id: range2115805
version: 1
top level: BBa_M1410
direction: direction/forward
start: 640
end: 642
orientation: inline

BBa_M1411
type: ComponentDefinition
persistent identity: BBa M1411
display id: BBa_M1411
version: 1
was generated by: iGEM to SBOL conversion
description: ribE gene
created: 2011-04-08T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Escherichia coli str. K-12 substr. MG1655, complete genome.
owned by: james
owned by: myers
mutable description: Codes for enzyme 6,7-dimethyl-8-ribityllumazine synthase that converts 5-amino-6-(D-ribitylamino)uracil and 1-deoxy-L-glycero-tetrulose 4-phosphate into 6,7-dimethyl-8-(1-D-ribityl)lumazine. This biochemical pathway is in the formation of riboflavin or vitamin B2.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8963
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Sarah Prince
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1411_sequence/1

Coding
type: SequenceAnnotation
persistent identity: annotation2115809
display id: annotation2115809
version: 1
top level: BBa_M1411
location: https://synbiohub.org/public/igem/BBa_M1411/annotation2115809/range2115809/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1411/annotation2115809/range2115809/1
type: Range
persistent identity: range2115809
display id: range2115809
version: 1
top level: BBa_M1411
direction: direction/forward
start: 1
end: 471
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2115807
display id: annotation2115807
version: 1
top level: BBa_M1411
location: https://synbiohub.org/public/igem/BBa_M1411/annotation2115807/range2115807/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1411/annotation2115807/range2115807/1
type: Range
persistent identity: range2115807
display id: range2115807
version: 1
top level: BBa_M1411
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2115808
display id: annotation2115808
version: 1
top level: BBa_M1411
location: https://synbiohub.org/public/igem/BBa_M1411/annotation2115808/range2115808/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1411/annotation2115808/range2115808/1
type: Range
persistent identity: range2115808
display id: range2115808
version: 1
top level: BBa_M1411
direction: direction/forward
start: 469
end: 471
orientation: inline

BBa_M1440
type: ComponentDefinition
persistent identity: BBa M1440
display id: BBa_M1440
version: 1
was generated by: iGEM to SBOL conversion
description: ribB
created: 2011-04-18T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Escherichia coli K-12
owned by: james
owned by: myers
mutable description: RibB catalyzes the synthesis of the 4-carbon compound 3,4-dihydroxy-2-butanone-4-phosphate, a precursor of the terminal intermediate, 6,7-dimethyl-8-ribityllumazine, in the biosynthesis of riboflavin
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Candace Clark
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding Sequence
sequence annotation: Start
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1440_sequence/1

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2116399
display id: annotation2116399
version: 1
top level: BBa_M1440
location: https://synbiohub.org/public/igem/BBa_M1440/annotation2116399/range2116399/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1440/annotation2116399/range2116399/1
type: Range
persistent identity: range2116399
display id: range2116399
version: 1
top level: BBa_M1440
direction: direction/forward
start: 1
end: 654
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2116397
display id: annotation2116397
version: 1
top level: BBa_M1440
location: https://synbiohub.org/public/igem/BBa_M1440/annotation2116397/range2116397/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1440/annotation2116397/range2116397/1
type: Range
persistent identity: range2116397
display id: range2116397
version: 1
top level: BBa_M1440
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2116398
display id: annotation2116398
version: 1
top level: BBa_M1440
location: https://synbiohub.org/public/igem/BBa_M1440/annotation2116398/range2116398/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1440/annotation2116398/range2116398/1
type: Range
persistent identity: range2116398
display id: range2116398
version: 1
top level: BBa_M1440
direction: direction/forward
start: 652
end: 654
orientation: inline

BBa_M1406
type: ComponentDefinition
persistent identity: BBa M1406
display id: BBa_M1406
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter + RBS
created: 2011-04-22T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: Promoter (J23119) + RBS (B0030) composite
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8954
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Asif Rahman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_M1406_sequence/1

BBa_B0030
type: Component
persistent identity: component2117037
display id: component2117037
version: 1
top level: BBa_M1406
definition: BBa_B0030
access: public

BBa_J23119
type: Component
persistent identity: component2117035
display id: component2117035
version: 1
top level: BBa_M1406
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2117035
display id: annotation2117035
version: 1
top level: BBa_M1406
location: https://synbiohub.org/public/igem/BBa_M1406/annotation2117035/range2117035/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_M1406/annotation2117035/range2117035/1
type: Range
persistent identity: range2117035
display id: range2117035
version: 1
top level: BBa_M1406
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2117037
display id: annotation2117037
version: 1
top level: BBa_M1406
location: https://synbiohub.org/public/igem/BBa_M1406/annotation2117037/range2117037/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_M1406/annotation2117037/range2117037/1
type: Range
persistent identity: range2117037
display id: range2117037
version: 1
top level: BBa_M1406
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

https://synbiohub.org/public/igem/BBa_M1408_sequence/1
type: Sequence
persistent identity: BBa M1408 sequence
display id: BBa_M1408_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtcagctggctttacgctcatgtgcctgacggtatgccaccaggtcttcaatagtcacgagcgccatattgtgtttattggcaaactcaatacactctggtgcacgcgccatcgtgccatcgtcattagtcagctcacacagtacaccagccggtttaaagcctgccagcgtcatcagatcaatagttgcttcagtatgaccgccacgcgtcagtacaccacctgcctgagcgcgaagtgggaaaacgtggccaggacgattcagatctgacggttttgcgccatcggcaatcgctgcgcgaacggtcgtaatacggtcagcggcagaaacaccggtagtcacaccttcagctgcttcaatggtcacggtaaaaccggtgccataggcgctggtgttattttctaccatcattggcagatcgagttgtttacggcgatcttcagtaatgcacaggcaaacaataccgctaccgtggcgaatggtcagcgccatctgctcaacagtcatggtttctgccgggaagatcatatcaccttcgttttcacggtcttcatcatcaagcaccattacaccgcgtccttcacgcagcgcagccagtgcattttcaacacgttcgaaaggcgtaccaaaagaggaaagtagcgtctgattcattactagagattaaagaggagaaatactagatgaacattattgaagctaacgttgctaccccggacgctcgcgtcgccatcaccatcgcgcgtttcaacaactttatcaatgacagcctgctggaaggtgcaattgacgcactgaaacgtatcggtcaggtaaaagatgaaaacattaccgttgtttgggtgcctggtgcctatgagctgccgctggcggcgggtgcactggctaaaaccggtaaatacgacgcggtgattgcgctgggtacggttattcgtggtggcactgcccactttgaatatgtcgctggtggtgcaagcaacggcctggcgcatgttgcccaggacagcgaaattccggttgcttttggggttctgaccactgaaagcattgaacaagcgatcgaacgtgctggcaccaaagctggcaacaaaggtgcagaagctgcactgaccgcgcttgaaatgattaatgtattgaaagccatcaaggcctgatactagagattaaagaggagaaatactagagtcaggcttctgtgcctggttgattcatggcattttctcgtgccgccagcacacgttctaccgtatctaccactgcctgagtttgtggatcgatttcaatgttgacgcgtgcgccaagttttttcttcccaagagtcgtgcgttccagtgtttccggaattaaatggacgcaaaaacgcgttggcgtgacttcgccgacggtcaggctaataccgtcgatgccaataaatcctttgtacagaatatatttcatcaactgactatcctggactttaaaccagatctggcgattattttctgaggttaatattttcgccacttcagcagtggtcataatatgacctgacattaagtgtccgccaatttcatcactgaatttcgccgcacgctcaacgtttacccaatcccccacttttaaatcgccaagattggtaatgcgtaacgtttctttcatcaggtcaaaactgacatggttgccgttaatttccgtcacggtcaggcagcaaccgttatgcgccacggaagcaccggtttccaggccgtccagcatgtggtcgggtaactccaccacatgcgtacgaaaatttggtttctcgtcaatcgacaccagttttgcggtgccctgtacaatccccgtaaacattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1410_sequence/1
type: Sequence
persistent identity: BBa M1410 sequence
display id: BBa_M1410_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaggcttctgtgcctggttgattcatggcattttctcgtgccgccagcacacgttctaccgtatctaccactgcctgagtttgtggatcgatttcaatgttgacgcgtgcgccaagttttttcttcccaagagtcgtgcgttccagtgtttccggaattaaatggacgcaaaaacgcgttggcgtgacttcgccgacggtcaggctaataccgtcgatgccaataaatcctttgtacagaatatatttcatcaactgactatcctggactttaaaccagatctggcgattattttctgaggttaatattttcgccacttcagcagtggtcataatatgacctgacattaagtgtccgccaatttcatcactgaatttcgccgcacgctcaacgtttacccaatcccccacttttaaatcgccaagattggtaatgcgtaacgtttctttcatcaggtcaaaactgacatggttgccgttaatttccgtcacggtcaggcagcaaccgttatgcgccacggaagcaccggtttccaggccgtccagcatgtggtcgggtaactccaccacatgcgtacgaaaatttggtttctcgtcaatcgacaccagttttgcggtgccctgtacaatccccgtaaacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1411_sequence/1
type: Sequence
persistent identity: BBa M1411 sequence
display id: BBa_M1411_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacattattgaagctaacgttgctaccccggacgctcgcgtcgccatcaccatcgcgcgtttcaacaactttatcaatgacagcctgctggaaggtgcaattgacgcactgaaacgtatcggtcaggtaaaagatgaaaacattaccgttgtttgggtgcctggtgcctatgagctgccgctggcggcgggtgcactggctaaaaccggtaaatacgacgcggtgattgcgctgggtacggttattcgtggtggcactgcccactttgaatatgtcgctggtggtgcaagcaacggcctggcgcatgttgcccaggacagcgaaattccggttgcttttggggttctgaccactgaaagcattgaacaagcgatcgaacgtgctggcaccaaagctggcaacaaaggtgcagaagctgcactgaccgcgcttgaaatgattaatgtattgaaagccatcaaggcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1440_sequence/1
type: Sequence
persistent identity: BBa M1440 sequence
display id: BBa_M1440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagctggctttacgctcatgtgcctgacggtatgccaccaggtcttcaatagtcacgagcgccatattgtgtttattggcaaactcaatacactctggtgcacgcgccatcgtgccatcgtcattagtcagctcacacagtacaccagccggtttaaagcctgccagcgtcatcagatcaatagttgcttcagtatgaccgccacgcgtcagtacaccacctgcctgagcgcgaagtgggaaaacgtggccaggacgattcagatctgacggttttgcgccatcggcaatcgctgcgcgaacggtcgtaatacggtcagcggcagaaacaccggtagtcacaccttcagctgcttcaatggtcacggtaaaaccggtgccataggcgctggtgttattttctaccatcattggcagatcgagttgtttacggcgatcttcagtaatgcacaggcaaacaataccgctaccgtggcgaatggtcagcgccatctgctcaacagtcatggtttctgccgggaagatcatatcaccttcgttttcacggtcttcatcatcaagcaccattacaccgcgtccttcacgcagcgcagccagtgcattttcaacacgttcgaaaggcgtaccaaaagaggaaagtagcgtctgattcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1406_sequence/1
type: Sequence
persistent identity: BBa M1406 sequence
display id: BBa_M1406_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z