LOCUS BBa_M1420 5579 bp DNA linear 22-Dec-2024 DEFINITION Riboflavin Gene Circuit ACCESSION BBa_M1420 VERSION BBa_M1420.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 1..58 /label=BBa_M1406 CDS 67..1601 /label=cds misc_feature 72..74 /label=start misc_feature 1537..1539 /label=stop CDS 67..1601 /label=BBa_M1405 RBS 1610..1624 /label=RBS-1\Strong RBS 1617..1621 /label=RBS misc_feature 1610..1624 /label=BBa_B0030 RBS 1610..1624 /label=BBa_B0030 misc_feature 1633..1635 /label=start misc_feature 2734..2736 /label=stop CDS 1633..2736 /label=cds CDS 1633..2736 /label=BBa_M1409 RBS 2745..2759 /label=RBS-1\Strong RBS 2752..2756 /label=RBS misc_feature 2745..2759 /label=BBa_B0030 RBS 2745..2759 /label=BBa_B0030 misc_feature 2766..2768 /label=Start CDS 2766..3584 /label=Coding misc_feature 3582..3584 /label=Stop CDS 2766..3584 /label=BBa_M1441 RBS 3593..3607 /label=RBS-1\Strong RBS 3600..3604 /label=RBS misc_feature 3593..3607 /label=BBa_B0030 RBS 3593..3607 /label=BBa_B0030 misc_feature 3616..3618 /label=Start misc_feature 4267..4269 /label=Stop CDS 3616..4269 /label=Coding Sequence CDS 3616..4269 /label=BBa_M1440 RBS 4278..4292 /label=RBS-1\Strong RBS 4285..4289 /label=RBS misc_feature 4278..4292 /label=BBa_B0030 RBS 4278..4292 /label=BBa_B0030 misc_feature 4299..4301 /label=Start misc_feature 4767..4769 /label=Stop CDS 4299..4769 /label=Coding CDS 4299..4769 /label=BBa_M1411 RBS 4778..4792 /label=RBS-1\Strong RBS 4785..4789 /label=RBS misc_feature 4778..4792 /label=BBa_B0030 RBS 4778..4792 /label=BBa_B0030 misc_feature 4801..4803 /label=Start misc_feature 5440..5442 /label=Stop CDS 4801..5442 /label=Coding CDS 4801..5442 /label=BBa_M1410 stem_loop 5462..5505 /label=stem_loop misc_feature 5451..5530 /label=BBa_B0010 terminator 5451..5530 /label=BBa_B0010 stem_loop 5546..5565 /label=T7 TE misc_feature 5572..5572 /label=stop polyA_site 5566..5579 /label=polya misc_feature 5539..5579 /label=BBa_B0012 terminator 5539..5579 /label=BBa_B0012 terminator 5451..5579 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata 61 ctagagttcc catgactccc gttgcccaga ccagcaagat agcaatggtt aacgttcgcc 121 gacgcggcca cagcaaacca acggccagca gtgcccaact ggcagcaaac atcgtgtgac 181 cggaaggaaa ggcaaacccc gtctctttct gccagtgtga acgcaaatat tgtgggatat 241 ttttctcttc agccaactgt tctttcacta gatttccgcg ttctgctcgc tttaaagtgt 301 agaactcatc aaccggaata tgatgtgttt tttccagcca gataacaaaa ggtcgtggtt 361 cctggacttt gtctttgatc caggatttaa cgccttgtcc cacaaggatt gcggccgcca 421 gaatggcaaa taatacaaag gcagccttaa tgcgaaaacg cagacaccag agaaaccagc 481 cgaataaaat caaatgtgta atgacgcccc agggctgggt gacagtttca gtaacccaaa 541 aagccgcttt tagtagccaa ctttgttctc caggttgcca acgccagcca gaaatccata 601 cggctactgg catgacaagc aatagtgcag ctcccactgc ggtacgtctg gcaatcgaac 661 gcatggcctc tcctttttga taagtcccac aatcataact gaaaacgcca gttccaggaa 721 aaattgacag atttgtgcca ttccgtgaac gatcgacgcg tcgtgattag gtgaacccct 781 tctcgttatg gcaaaataag ccaatacaga accagcatta tctggagaat ttcatgcagc 841 ttaaacgtgt ggcagaagcc aaactgccaa ccccatgggg cgatttcctg atggtgggat 901 ttgaagaact ggcaaccgga cacgatcatg tcgcgctagt ctatggcgat atttccgggc 961 ataccccggt acttgcgcgc gtccattccg aatgtctgac cggtgacgcc ctgttcagct 1021 tgcgctgcga ttgtggcttc cagctcgaag cggcattgac gcaaattgcc gaggaaggcc 1081 gtggtatttt gctgtatcac cgtcaggaag gtcgtaacat tggtctgctg aataaaatcc 1141 gcgcttacgc actgtaggat caaggttacg ataccgtaga ggctaaccac cagttaggct 1201 tcgccgctga tgagcgcgac ttcactcttt gcgctgatat gttcaaactc cttggcgtca 1261 atgaagtccg cttgttaacc aataacccga aaaaagtcga aattctgacc gaagcaggga 1321 ttaatattgt tgaacgcgta ccattgattg taggtcgtaa ccccaataac gaacattatc 1381 tcgataccaa agccgagaaa atgggccatt tgctgaacaa ataaccctct tgcattgtgt 1441 aattcatttg cttgccggaa gcaaaataac cggcaagcaa atagttgtta cttcaacata 1501 ttacgaatga cataatgcaa aatgccgtcg ttctggtagt aggtcaactc cgtcgcggtg 1561 tcgatacgac aacggcaggg tacgacttcc tggctaccat ctactagaga ttaaagagga 1621 gaaatactag aggtgcagga cgagtattac atggcgcggg cgctaaagct ggcgcaacga 1681 ggacgtttta ccacgcatcc caacccgaat gtcgggtgcg tcattgtcaa agatggcgaa 1741 attgtcggtg aaggttacca ccaacgtgcg ggtgaaccac atgccgaagt acacgcgttg 1801 cgtatggcgg gtgaaaaagc caaaggtgcg accgcctatg tcacactcga accctgtagc 1861 catcatggtc gtacgccacc gtgctgtgac gcactcatcg ccgctggcgt agcgcgcgtg 1921 gttgcctcga tgcaagatcc taacccgcag gtcgctgggc gtggacttta ccgtctgcaa 1981 caggctggca ttgacgtcag ccacggcctg atgatgagtg aagccgagca attgaataaa 2041 ggctttctca agcggatgcg caccggcttt ccttatattc agttaaaact tggcgcatcg 2101 cttgatggtc gcacggcgat ggcgagcggc gaaagccagt ggatcacttc gccccaggcg 2161 cggcgtgatg tacaactact gcgcgcgcaa agtcatgcca ttttaaccag cagcgccacg 2221 gtgctggcgg atgatcctgc cttaacggtg cgttggtctg aactggatga acaaactcag 2281 gcgctctatc cgcaacaaaa tctccgtcag ccgatacgta ttgtgattga tagccaaaat 2341 cgcgtgacgc cggtacatcg cattgtgcag cagcccggcg aaacctggtt cgcgcgtacg 2401 caggaagatt ctcgtgagtg gccggaaacg gtgcgtacct tgctgattcc agagcataaa 2461 ggtcatctgg atctggttgt actgatgatg caactgggta aacagcaaat taacagcatc 2521 tgggtggaag cggggccaac gctcgctggc gcattgctgc aagcgggttt agtcgatgag 2581 ctgattgtct atatcgcacc taaactatta ggcagcgacg cccgcggatt atgcacgctg 2641 ccagggcttg agaaattagc cgacgccccc caatttaaat tcaaagagat acgtcatgta 2701 ggcccggatg tttgcctgca tttagtgggt gcatgatact agagattaaa gaggagaaat 2761 actagatggc tcgtctggca gcatttgata tggatggcac tttattgatg cccgaccatc 2821 atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac attaccctca 2881 cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg ctatcgctgg 2941 atgcgtattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt gaacttttac 3001 atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa tgggataccc 3061 gagccagcat gcatatcttc aatgacgacg gttggtttac cgggaaagag atccctgcgt 3121 tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc aaaaaaatgc 3181 cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt acacgcttgc 3241 agatccagct atacgaagca ttaggcgagc gtgcacattt gtgtttttcc gccacggatt 3301 gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc attgacggtg ctgacccaac 3361 atttaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa 3421 tgttagtcag cgtcggtagc ggatttatta tgggcaatgc gatgccgcaa ctgcgcgcgg 3481 agctcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct cactatttga 3541 cgcactggct ggactatcca catctacctt attcccccga ataatactag agattaaaga 3601 ggagaaatac tagagtcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc 3661 ttcaatagtc acgagcgcca tattgtgttt attggcaaac tcaatacact ctggtgcacg 3721 cgccatcgtg ccatcgtcat tagtcagctc acacagtaca ccagccggtt taaagcctgc 3781 cagcgtcatc agatcaatag ttgcttcagt atgaccgcca cgcgtcagta caccacctgc 3841 ctgagcgcga agtgggaaaa cgtggccagg acgattcaga tctgacggtt ttgcgccatc 3901 ggcaatcgct gcgcgaacgg tcgtaatacg gtcagcggca gaaacaccgg tagtcacacc 3961 ttcagctgct tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac 4021 catcattggc agatcgagtt gtttacggcg atcttcagta atgcacaggc aaacaatacc 4081 gctaccgtgg cgaatggtca gcgccatctg ctcaacagtc atggtttctg ccgggaagat 4141 catatcacct tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg 4201 cagcgcagcc agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt 4261 ctgattcatt actagagatt aaagaggaga aatactagat gaacattatt gaagctaacg 4321 ttgctacccc ggacgctcgc gtcgccatca ccatcgcgcg tttcaacaac tttatcaatg 4381 acagcctgct ggaaggtgca attgacgcac tgaaacgtat cggtcaggta aaagatgaaa 4441 acattaccgt tgtttgggtg cctggtgcct atgagctgcc gctggcggcg ggtgcactgg 4501 ctaaaaccgg taaatacgac gcggtgattg cgctgggtac ggttattcgt ggtggcactg 4561 cccactttga atatgtcgct ggtggtgcaa gcaacggcct ggcgcatgtt gcccaggaca 4621 gcgaaattcc ggttgctttt ggggttctga ccactgaaag cattgaacaa gcgatcgaac 4681 gtgctggcac caaagctggc aacaaaggtg cagaagctgc actgaccgcg cttgaaatga 4741 ttaatgtatt gaaagccatc aaggcctgat actagagatt aaagaggaga aatactagag 4801 tcaggcttct gtgcctggtt gattcatggc attttctcgt gccgccagca cacgttctac 4861 cgtatctacc actgcctgag tttgtggatc gatttcaatg ttgacgcgtg cgccaagttt 4921 tttcttccca agagtcgtgc gttccagtgt ttccggaatt aaatggacgc aaaaacgcgt 4981 tggcgtgact tcgccgacgg tcaggctaat accgtcgatg ccaataaatc ctttgtacag 5041 aatatatttc atcaactgac tatcctggac tttaaaccag atctggcgat tattttctga 5101 ggttaatatt ttcgccactt cagcagtggt cataatatga cctgacatta agtgtccgcc 5161 aatttcatca ctgaatttcg ccgcacgctc aacgtttacc caatccccca cttttaaatc 5221 gccaagattg gtaatgcgta acgtttcttt catcaggtca aaactgacat ggttgccgtt 5281 aatttccgtc acggtcaggc agcaaccgtt atgcgccacg gaagcaccgg tttccaggcc 5341 gtccagcatg tggtcgggta actccaccac atgcgtacga aaatttggtt tctcgtcaat 5401 cgacaccagt tttgcggtgc cctgtacaat ccccgtaaac attactagag ccaggcatca 5461 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 5521 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //