##gff-version 3
##sequence-region BBa_M1420 1 5579
BBa_M1420	.	ribosome_entry_site	4278	4292	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1420	.	engineered_region	4278	4292	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	4285	4289	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	4278	4292	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	4778	4792	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1420	.	engineered_region	4778	4792	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	4785	4789	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	4778	4792	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	CDS	4801	5442	.	+	0	ID=BBa_M1410;Name=BBa_M1410
BBa_M1420	.	CDS	4801	5442	.	+	0	ID=annotation2115806;Name=Coding;Parent=BBa_M1410
BBa_M1420	.	stop_codon	5440	5442	.	+	0	ID=annotation2115805;Name=Stop;Parent=BBa_M1410
BBa_M1420	.	start_codon	4801	4803	.	+	0	ID=annotation2115804;Name=Start;Parent=BBa_M1410
BBa_M1420	.	CDS	2766	3584	.	+	0	ID=BBa_M1441;Name=BBa_M1441
BBa_M1420	.	stop_codon	3582	3584	.	+	0	ID=annotation2117041;Name=Stop;Parent=BBa_M1441
BBa_M1420	.	CDS	2766	3584	.	+	0	ID=annotation2117040;Name=Coding;Parent=BBa_M1441
BBa_M1420	.	start_codon	2766	2768	.	+	0	ID=annotation2117039;Name=Start;Parent=BBa_M1441
BBa_M1420	.	terminator	5451	5579	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1420	.	terminator	5451	5530	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1420	.	stem_loop	5462	5505	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1420	.	engineered_region	5451	5530	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1420	.	terminator	5539	5579	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1420	.	engineered_region	5539	5579	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1420	.	stop_codon	5572	5572	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1420	.	stem_loop	5546	5565	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1420	.	polyA_site	5566	5579	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1420	.	CDS	4299	4769	.	+	0	ID=BBa_M1411;Name=BBa_M1411
BBa_M1420	.	start_codon	4299	4301	.	+	0	ID=annotation2115807;Name=Start;Parent=BBa_M1411
BBa_M1420	.	stop_codon	4767	4769	.	+	0	ID=annotation2115808;Name=Stop;Parent=BBa_M1411
BBa_M1420	.	CDS	4299	4769	.	+	0	ID=annotation2115809;Name=Coding;Parent=BBa_M1411
BBa_M1420	.	CDS	67	1601	.	+	0	ID=BBa_M1405;Name=BBa_M1405
BBa_M1420	.	stop_codon	1537	1539	.	+	0	ID=annotation2117033;Name=stop;Parent=BBa_M1405
BBa_M1420	.	start_codon	72	74	.	+	0	ID=annotation2117032;Name=start;Parent=BBa_M1405
BBa_M1420	.	CDS	67	1601	.	+	0	ID=annotation2117031;Name=cds;Parent=BBa_M1405
BBa_M1420	.	ribosome_entry_site	1610	1624	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1420	.	engineered_region	1610	1624	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	1617	1621	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	1610	1624	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	engineered_region	1	58	.	+	0	ID=BBa_M1406;Name=BBa_M1406
BBa_M1420	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_M1406
BBa_M1420	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_M1406
BBa_M1420	.	ribosome_entry_site	3593	3607	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1420	.	engineered_region	3593	3607	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	3600	3604	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	3593	3607	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	2745	2759	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1420	.	engineered_region	2745	2759	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	2752	2756	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1420	.	ribosome_entry_site	2745	2759	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1420	.	CDS	3616	4269	.	+	0	ID=BBa_M1440;Name=BBa_M1440
BBa_M1420	.	stop_codon	4267	4269	.	+	0	ID=annotation2116398;Name=Stop;Parent=BBa_M1440
BBa_M1420	.	CDS	3616	4269	.	+	0	ID=annotation2116399;Name=Coding Sequence ;Parent=BBa_M1440
BBa_M1420	.	start_codon	3616	3618	.	+	0	ID=annotation2116397;Name=Start;Parent=BBa_M1440
BBa_M1420	.	CDS	1633	2736	.	+	0	ID=BBa_M1409;Name=BBa_M1409
BBa_M1420	.	stop_codon	2734	2736	.	+	0	ID=annotation2117077;Name=stop;Parent=BBa_M1409
BBa_M1420	.	CDS	1633	2736	.	+	0	ID=annotation2117078;Name=cds;Parent=BBa_M1409
BBa_M1420	.	start_codon	1633	1635	.	+	0	ID=annotation2117076;Name=start;Parent=BBa_M1409
>BBa_M1420
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagttcccatgactcc
cgttgcccagaccagcaagatagcaatggttaacgttcgccgacgcggccacagcaaaccaacggccagcagtgcccaa
ctggcagcaaacatcgtgtgaccggaaggaaaggcaaaccccgtctctttctgccagtgtgaacgcaaatattgtggga
tatttttctcttcagccaactgttctttcactagatttccgcgttctgctcgctttaaagtgtagaactcatcaaccgg
aatatgatgtgttttttccagccagataacaaaaggtcgtggttcctggactttgtctttgatccaggatttaacgcct
tgtcccacaaggattgcggccgccagaatggcaaataatacaaaggcagccttaatgcgaaaacgcagacaccagagaa
accagccgaataaaatcaaatgtgtaatgacgccccagggctgggtgacagtttcagtaacccaaaaagccgcttttag
tagccaactttgttctccaggttgccaacgccagccagaaatccatacggctactggcatgacaagcaatagtgcagct
cccactgcggtacgtctggcaatcgaacgcatggcctctcctttttgataagtcccacaatcataactgaaaacgccag
ttccaggaaaaattgacagatttgtgccattccgtgaacgatcgacgcgtcgtgattaggtgaaccccttctcgttatg
gcaaaataagccaatacagaaccagcattatctggagaatttcatgcagcttaaacgtgtggcagaagccaaactgcca
accccatggggcgatttcctgatggtgggatttgaagaactggcaaccggacacgatcatgtcgcgctagtctatggcg
atatttccgggcataccccggtacttgcgcgcgtccattccgaatgtctgaccggtgacgccctgttcagcttgcgctg
cgattgtggcttccagctcgaagcggcattgacgcaaattgccgaggaaggccgtggtattttgctgtatcaccgtcag
gaaggtcgtaacattggtctgctgaataaaatccgcgcttacgcactgtaggatcaaggttacgataccgtagaggcta
accaccagttaggcttcgccgctgatgagcgcgacttcactctttgcgctgatatgttcaaactccttggcgtcaatga
agtccgcttgttaaccaataacccgaaaaaagtcgaaattctgaccgaagcagggattaatattgttgaacgcgtacca
ttgattgtaggtcgtaaccccaataacgaacattatctcgataccaaagccgagaaaatgggccatttgctgaacaaat
aaccctcttgcattgtgtaattcatttgcttgccggaagcaaaataaccggcaagcaaatagttgttacttcaacatat
tacgaatgacataatgcaaaatgccgtcgttctggtagtaggtcaactccgtcgcggtgtcgatacgacaacggcaggg
tacgacttcctggctaccatctactagagattaaagaggagaaatactagaggtgcaggacgagtattacatggcgcgg
gcgctaaagctggcgcaacgaggacgttttaccacgcatcccaacccgaatgtcgggtgcgtcattgtcaaagatggcg
aaattgtcggtgaaggttaccaccaacgtgcgggtgaaccacatgccgaagtacacgcgttgcgtatggcgggtgaaaa
agccaaaggtgcgaccgcctatgtcacactcgaaccctgtagccatcatggtcgtacgccaccgtgctgtgacgcactc
atcgccgctggcgtagcgcgcgtggttgcctcgatgcaagatcctaacccgcaggtcgctgggcgtggactttaccgtc
tgcaacaggctggcattgacgtcagccacggcctgatgatgagtgaagccgagcaattgaataaaggctttctcaagcg
gatgcgcaccggctttccttatattcagttaaaacttggcgcatcgcttgatggtcgcacggcgatggcgagcggcgaa
agccagtggatcacttcgccccaggcgcggcgtgatgtacaactactgcgcgcgcaaagtcatgccattttaaccagca
gcgccacggtgctggcggatgatcctgccttaacggtgcgttggtctgaactggatgaacaaactcaggcgctctatcc
gcaacaaaatctccgtcagccgatacgtattgtgattgatagccaaaatcgcgtgacgccggtacatcgcattgtgcag
cagcccggcgaaacctggttcgcgcgtacgcaggaagattctcgtgagtggccggaaacggtgcgtaccttgctgattc
cagagcataaaggtcatctggatctggttgtactgatgatgcaactgggtaaacagcaaattaacagcatctgggtgga
agcggggccaacgctcgctggcgcattgctgcaagcgggtttagtcgatgagctgattgtctatatcgcacctaaacta
ttaggcagcgacgcccgcggattatgcacgctgccagggcttgagaaattagccgacgccccccaatttaaattcaaag
agatacgtcatgtaggcccggatgtttgcctgcatttagtgggtgcatgatactagagattaaagaggagaaatactag
atggctcgtctggcagcatttgatatggatggcactttattgatgcccgaccatcatttaggtgagaaaaccctctcta
ctttggcgcgactgcgtgaacgcgacattaccctcacttttgccacggggcgtcatgcgctggagatgcagcatattct
cggggcgctatcgctggatgcgtatttgattaccggcaacggaacgcgcgtgcattctctggaaggtgaacttttacat
cgtgatgatttacctgcggatgtcgcggagctggtgctgtatcagcaatgggatacccgagccagcatgcatatcttca
atgacgacggttggtttaccgggaaagagatccctgcgttgttgcaggcatttgtctatagcggttttcgttatcagat
aatcgatgtcaaaaaaatgccactcggcagcgtcaccaagatctgcttctgtggcgatcacgacgatcttacacgcttg
cagatccagctatacgaagcattaggcgagcgtgcacatttgtgtttttccgccacggattgcctcgaagtgctgccgg
tgggctgcaataaaggcgctgcattgacggtgctgacccaacatttaggtttatcgttgcgcgattgcatggcctttgg
tgatgcgatgaacgatcgcgaaatgttagtcagcgtcggtagcggatttattatgggcaatgcgatgccgcaactgcgc
gcggagctcccgcatttaccggtgattggacattgccgaaatcaggctgtctctcactatttgacgcactggctggact
atccacatctaccttattcccccgaataatactagagattaaagaggagaaatactagagtcagctggctttacgctca
tgtgcctgacggtatgccaccaggtcttcaatagtcacgagcgccatattgtgtttattggcaaactcaatacactctg
gtgcacgcgccatcgtgccatcgtcattagtcagctcacacagtacaccagccggtttaaagcctgccagcgtcatcag
atcaatagttgcttcagtatgaccgccacgcgtcagtacaccacctgcctgagcgcgaagtgggaaaacgtggccagga
cgattcagatctgacggttttgcgccatcggcaatcgctgcgcgaacggtcgtaatacggtcagcggcagaaacaccgg
tagtcacaccttcagctgcttcaatggtcacggtaaaaccggtgccataggcgctggtgttattttctaccatcattgg
cagatcgagttgtttacggcgatcttcagtaatgcacaggcaaacaataccgctaccgtggcgaatggtcagcgccatc
tgctcaacagtcatggtttctgccgggaagatcatatcaccttcgttttcacggtcttcatcatcaagcaccattacac
cgcgtccttcacgcagcgcagccagtgcattttcaacacgttcgaaaggcgtaccaaaagaggaaagtagcgtctgatt
cattactagagattaaagaggagaaatactagatgaacattattgaagctaacgttgctaccccggacgctcgcgtcgc
catcaccatcgcgcgtttcaacaactttatcaatgacagcctgctggaaggtgcaattgacgcactgaaacgtatcggt
caggtaaaagatgaaaacattaccgttgtttgggtgcctggtgcctatgagctgccgctggcggcgggtgcactggcta
aaaccggtaaatacgacgcggtgattgcgctgggtacggttattcgtggtggcactgcccactttgaatatgtcgctgg
tggtgcaagcaacggcctggcgcatgttgcccaggacagcgaaattccggttgcttttggggttctgaccactgaaagc
attgaacaagcgatcgaacgtgctggcaccaaagctggcaacaaaggtgcagaagctgcactgaccgcgcttgaaatga
ttaatgtattgaaagccatcaaggcctgatactagagattaaagaggagaaatactagagtcaggcttctgtgcctggt
tgattcatggcattttctcgtgccgccagcacacgttctaccgtatctaccactgcctgagtttgtggatcgatttcaa
tgttgacgcgtgcgccaagttttttcttcccaagagtcgtgcgttccagtgtttccggaattaaatggacgcaaaaacg
cgttggcgtgacttcgccgacggtcaggctaataccgtcgatgccaataaatcctttgtacagaatatatttcatcaac
tgactatcctggactttaaaccagatctggcgattattttctgaggttaatattttcgccacttcagcagtggtcataa
tatgacctgacattaagtgtccgccaatttcatcactgaatttcgccgcacgctcaacgtttacccaatcccccacttt
taaatcgccaagattggtaatgcgtaacgtttctttcatcaggtcaaaactgacatggttgccgttaatttccgtcacg
gtcaggcagcaaccgttatgcgccacggaagcaccggtttccaggccgtccagcatgtggtcgggtaactccaccacat
gcgtacgaaaatttggtttctcgtcaatcgacaccagttttgcggtgccctgtacaatccccgtaaacattactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata