Composes all of the enzymes needed in the biological pathway to create riboflavin in E. coli.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
RibB catalyzes the synthesis of the 4-carbon compound 3,4-dihydroxy-2-butanone-4-phosphate, a precursor of the terminal intermediate, 6,7-dimethyl-8-ribityllumazine, in the biosynthesis of riboflavin
rib A gene, first gene used in the production of vitamin B2. Converts GTP to 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one. E.coli ribA gene for GTP cyclohydrolase II.
It has an illegal PstI site between 1087 to 1093, which has been mutated out.
Promoter (J23119) + RBS (B0030) composite
cof thiamin pyrimidine pyrophosphate hydrolase
Escherichia coli str. K-12 substr. MG1655 Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF. Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada.
Dephosphorylation of phosphoric acid ester. Shows high catalytic efficiency and affinity to a wide range of phosphorylated metabolites that are intermediates of various metabolic reactions.
have to remove one PstI site from the gene
Double terminator consisting of BBa_B0010 and BBa_B0012
Escherichia coli K-12 MG1655 genome, ribC-pykF region.
Codes for the enzyme ribflavin synthase that converts 6,7-dimethyl-8-(1-D-ribityl)lumazine to riboflavin, or vitamin B2.
Escherichia coli str. K-12 substr. MG1655, complete genome.
Codes for enzyme 6,7-dimethyl-8-ribityllumazine synthase that converts 5-amino-6-(D-ribitylamino)uracil and 1-deoxy-L-glycero-tetrulose 4-phosphate into 6,7-dimethyl-8-(1-D-ribityl)lumazine. This biochemical pathway is in the formation of riboflavin or vitamin B2.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Overlap extension of synthetic oligonucleotides
Conversion of the iGEM parts registry to SBOL2.1