LOCUS       BBa_M1457               2786 bp    DNA     linear       23-Dec-2024
DEFINITION  Opioid + transmembrane
ACCESSION   BBa_M1457
VERSION     BBa_M1457.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    906..906
                     /label=I281S T->G
     misc_feature    1415..1417
                     /label=TAA
     misc_feature    65..67
                     /label=ATG
     CDS             65..1417
                     /label=BBa_K136046
     RBS             1426..1440
                     /label=RBS-1\Strong
     RBS             1433..1437
                     /label=RBS
     misc_feature    1426..1440
                     /label=BBa_B0030
     RBS             1426..1440
                     /label=BBa_B0030
     misc_feature    1447..1449
                     /label=Start
     CDS             1450..2646
                     /label=Coding
     misc_feature    2647..2649
                     /label=Stop
     CDS             1447..2649
                     /label=BBa_M1456
     stem_loop       2669..2712
                     /label=stem_loop
     misc_feature    2658..2737
                     /label=BBa_B0010
     terminator      2658..2737
                     /label=BBa_B0010
     stem_loop       2753..2772
                     /label=T7 TE
     misc_feature    2779..2779
                     /label=stop
     polyA_site      2773..2786
                     /label=polya
     misc_feature    2746..2786
                     /label=BBa_B0012
     terminator      2746..2786
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgagg cgattgcgct tctcgccacg aagttcattt gcccgtacgt tattgctcat
      121 cgtcaccttg ctgttcgcca gcctggtgac gacttatctg gtggtgctga acttcgcgat
      181 tttgccgagc ctccagcagt ttaataaagt cctcgcgtac gaagtgcgta tgttgatgac
      241 cgacaaactg caactggagg acggcacgca gttggttgtg cctcccgctt tccgtcggga
      301 gatctaccgt gagctgggga tctctctcta ctccaacgag gctgccgaag aggcaggtct
      361 gcgttgggcg caacactatg aattcttaag ccatcagatg gcgcagcaac tgggcggccc
      421 gacggaagtg cgcgttgagg tcaacaaaag ttcgcctgtc gtctggctga aaacctggct
      481 gtcgcccaat atctgggtac gcgtgccgct gaccgaaatt catcagggcg atttctctcc
      541 gctgttccgc tatacgctgg cgattatgct attggcgata ggcggggcgt ggctgtttat
      601 tcgtatccag aaccgaccgt tggtcgatct cgaacacgca gccttgcagg ttggtaaagg
      661 gattattccg ccgccgctgc gtgagtatgg cgcttcggag gtgcgttccg ttacccgtgc
      721 ctttaaccat atggcggctg gtgttaagca actggcggat gaccgcacgc tgctgatggc
      781 gggggtaagt cacgacttgc gcacgccgct gacgcgtatt cgcctggcga ctgagatgat
      841 gagcgagcag gatggctatc tggcagaatc gatcaataaa gatatcgaag agtgcaacgc
      901 catcagtgag cagtttatcg actacctgcg caccgggcag gagatgccga tggaaatggc
      961 ggatcttaat gcagtactcg gtgaggtgat tgctgccgaa agtggctatg agcgggaaat
     1021 tgaaaccgcg ctttaccccg gcagcattga agtgaaaatg cacccgctgt cgatcaaacg
     1081 cgcggtggcg aatatggtgg tcaacgccgc ccgttatggc aatggctgga tcaaagtcag
     1141 cagcggaacg gagccgaatc gcgcctggtt ccaggtggaa gatgacggtc cgggaattgc
     1201 gccggaacaa cgtaagcacc tgttccagcc gtttgtccgc ggcgacagtg cgcgcaccat
     1261 tagcggcacg ggattagggc tggcaattgt gcagcgtatc gtggataacc ataacgggat
     1321 gctggagctt ggcaccagcg agcggggcgg gctttccatt cgcgcctggc tgccagtgcc
     1381 ggtaacgcgg gcgcagggca cgacaaaaga agggtaatac tagagattaa agaggagaaa
     1441 tactagatgg atagctccgc agcacctacg aacgcatcca actgcaccga tgcgttagcg
     1501 tactcatcct gttcgcccgc accaagtccc ggttcatggg taaacctgtc gcatctggat
     1561 ggtaacttga gcgacccatg cggtccgaat cgaacagacc tgggtggtcg tgatagcctt
     1621 tgcccgccga caggttcacc gtctatgata accgcaatca caattatggc gctatacagt
     1681 attgtctgcg ttgtgggcct ttttggcaat tttctggtta tgtatgtcat tgtccggtac
     1741 accaaaatga agaccgccac gaatatttac atttttaatt tagcattagc ggatgccctg
     1801 gctacatcaa ccctgccatt tcaatctgtc aattatctca tggggacatg gccttttggt
     1861 actatcctgt gcaagatcgt tattagtatt gattattata acatgtttac ctctattttc
     1921 accctttgca ccatgtcagt tgatcgatat atagctgtat gtcatcccgt caaagcgtta
     1981 gactttcgga ctcctcggaa tgccaaaatc ataaacgtgt gcaattggat actgagttca
     2041 gcaatcggct tgcctgtcat gttcatggca acgacgaaat atcgtcaagg gagcatcgat
     2101 tgcacactaa cattctccca tccgacctgg tactgggaaa atctgctgaa gatatgtgtt
     2161 ttcatatttg catttatcat gccggtgctg attatcaccg tctgctatgg actgatgatt
     2221 ttacgcctta aatcggtgcg aatgctgtcc ggttctaaag aaaaggatag aaatttgaga
     2281 agaattacgc ggatggtgtt agtggttgtg gctgtgttca ttgtctgctg gacccctatc
     2341 catatctacg taataattaa agctctggtg acaatcccgg aaacaacgtt tcagacagta
     2401 tcatggcact tttgtatagc cctaggatat acaaatagct gcttaaaccc tgtactgtac
     2461 gcctttctgg atgaaaactt caagcgctgt tttcgtgagt tttgtatccc cactagcagt
     2521 aatatcgaac agcagaatag cacacgcatt cgtcagaata cccgcgacca cccctcgaca
     2581 gccaacacgg ttgaccgtac aaatcatcag ttagaaaact tagaagcgga gacggcgcct
     2641 ctcccgtgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2701 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2761 ttcgggtggg cctttctgcg tttata
//