BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_M1457
1
BBa_M1457
Opioid + transmembrane
2012-04-23T11:00:00Z
2015-05-08T01:13:56Z
From existing basic parts
Human opioid receptor fusion with EnvZ trnasmembrane protein
false
false
_768_
0
10129
9
Not in stock
false
Some may need to have mutagenesis PCR reactions to remove illegal EcoR1 sites
false
Eric B Anderson
component2173121
1
BBa_B0030
component2173127
1
BBa_B0010
component2173119
1
BBa_K136046
component2173126
1
BBa_M1456
component2173129
1
BBa_B0012
component2173114
1
BBa_B0030
component2173112
1
BBa_J23100
annotation2173126
1
BBa_M1456
range2173126
1
1447
2649
annotation2173129
1
BBa_B0012
range2173129
1
2746
2786
annotation2173114
1
BBa_B0030
range2173114
1
44
58
annotation2173121
1
BBa_B0030
range2173121
1
1426
1440
annotation2173119
1
BBa_K136046
range2173119
1
65
1417
annotation2173127
1
BBa_B0010
range2173127
1
2658
2737
annotation2173112
1
BBa_J23100
range2173112
1
1
35
BBa_K136046
1
BBa_K136046
envZ*
2008-10-24T11:00:00Z
2015-05-08T01:10:03Z
bacterial genome of mutated (I281S) strain for EnvZ.
EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
false
false
_211_
0
3215
9
It's complicated
false
PCR and ligation in pCR4Blunt-TOPO
false
alexandra bouaziz
annotation1994303
1
TAA
range1994303
1
1351
1353
annotation1994273
1
I281S T->G
range1994273
1
842
842
annotation1994304
1
ATG
range1994304
1
1
3
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_M1456
1
BBa_M1456
Mu Opioid receptor (human)
2012-04-23T11:00:00Z
2015-05-08T01:13:56Z
From aa sequence found on NCBI, attr. to Mestek, A. et al.
Backtranslated human opioid receptor (mu type). It has been found to react with prolamines like gliadins (subunits of wheat protein gluten)
false
false
_768_
0
10129
9
Not in stock
false
E. Coli stop codon TGA added in silico to the end of the coding sequence.
false
Eric B Anderson
annotation2173109
1
Start
range2173109
1
1
3
annotation2173110
1
Coding
range2173110
1
4
1200
annotation2173111
1
Stop
range2173111
1
1201
1203
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M1457_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagattaaagaggagaaatactagatggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K136046_sequence
1
atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M1456_sequence
1
atggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z