BBa_M1457 1 BBa_M1457 Opioid + transmembrane 2012-04-23T11:00:00Z 2015-05-08T01:13:56Z From existing basic parts Human opioid receptor fusion with EnvZ trnasmembrane protein false false _768_ 0 10129 9 Not in stock false Some may need to have mutagenesis PCR reactions to remove illegal EcoR1 sites false Eric B Anderson component2173119 1 BBa_K136046 component2173129 1 BBa_B0012 component2173112 1 BBa_J23100 component2173127 1 BBa_B0010 component2173114 1 BBa_B0030 component2173121 1 BBa_B0030 component2173126 1 BBa_M1456 annotation2173121 1 BBa_B0030 range2173121 1 1426 1440 annotation2173114 1 BBa_B0030 range2173114 1 44 58 annotation2173126 1 BBa_M1456 range2173126 1 1447 2649 annotation2173127 1 BBa_B0010 range2173127 1 2658 2737 annotation2173119 1 BBa_K136046 range2173119 1 65 1417 annotation2173129 1 BBa_B0012 range2173129 1 2746 2786 annotation2173112 1 BBa_J23100 range2173112 1 1 35 BBa_K136046 1 BBa_K136046 envZ* 2008-10-24T11:00:00Z 2015-05-08T01:10:03Z bacterial genome of mutated (I281S) strain for EnvZ. EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal. false false _211_ 0 3215 9 It's complicated false PCR and ligation in pCR4Blunt-TOPO false alexandra bouaziz annotation1994303 1 TAA range1994303 1 1351 1353 annotation1994304 1 ATG range1994304 1 1 3 annotation1994273 1 I281S T->G range1994273 1 842 842 BBa_M1456 1 BBa_M1456 Mu Opioid receptor (human) 2012-04-23T11:00:00Z 2015-05-08T01:13:56Z From aa sequence found on NCBI, attr. to Mestek, A. et al. Backtranslated human opioid receptor (mu type). It has been found to react with prolamines like gliadins (subunits of wheat protein gluten) false false _768_ 0 10129 9 Not in stock false E. Coli stop codon TGA added in silico to the end of the coding sequence. false Eric B Anderson annotation2173111 1 Stop range2173111 1 1201 1203 annotation2173109 1 Start range2173109 1 1 3 annotation2173110 1 Coding range2173110 1 4 1200 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M1457_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagattaaagaggagaaatactagatggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_K136046_sequence 1 atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M1456_sequence 1 atggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z