LOCUS       BBa_M1458               1404 bp    DNA     linear       23-Dec-2024
DEFINITION  Opioid constitutive
ACCESSION   BBa_M1458
VERSION     BBa_M1458.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    65..67
                     /label=Start
     CDS             68..1264
                     /label=Coding
     misc_feature    1265..1267
                     /label=Stop
     CDS             65..1267
                     /label=BBa_M1456
     stem_loop       1287..1330
                     /label=stem_loop
     misc_feature    1276..1355
                     /label=BBa_B0010
     terminator      1276..1355
                     /label=BBa_B0010
     stem_loop       1371..1390
                     /label=T7 TE
     misc_feature    1397..1397
                     /label=stop
     polyA_site      1391..1404
                     /label=polya
     misc_feature    1364..1404
                     /label=BBa_B0012
     terminator      1364..1404
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggat agctccgcag cacctacgaa cgcatccaac tgcaccgatg cgttagcgta
      121 ctcatcctgt tcgcccgcac caagtcccgg ttcatgggta aacctgtcgc atctggatgg
      181 taacttgagc gacccatgcg gtccgaatcg aacagacctg ggtggtcgtg atagcctttg
      241 cccgccgaca ggttcaccgt ctatgataac cgcaatcaca attatggcgc tatacagtat
      301 tgtctgcgtt gtgggccttt ttggcaattt tctggttatg tatgtcattg tccggtacac
      361 caaaatgaag accgccacga atatttacat ttttaattta gcattagcgg atgccctggc
      421 tacatcaacc ctgccatttc aatctgtcaa ttatctcatg gggacatggc cttttggtac
      481 tatcctgtgc aagatcgtta ttagtattga ttattataac atgtttacct ctattttcac
      541 cctttgcacc atgtcagttg atcgatatat agctgtatgt catcccgtca aagcgttaga
      601 ctttcggact cctcggaatg ccaaaatcat aaacgtgtgc aattggatac tgagttcagc
      661 aatcggcttg cctgtcatgt tcatggcaac gacgaaatat cgtcaaggga gcatcgattg
      721 cacactaaca ttctcccatc cgacctggta ctgggaaaat ctgctgaaga tatgtgtttt
      781 catatttgca tttatcatgc cggtgctgat tatcaccgtc tgctatggac tgatgatttt
      841 acgccttaaa tcggtgcgaa tgctgtccgg ttctaaagaa aaggatagaa atttgagaag
      901 aattacgcgg atggtgttag tggttgtggc tgtgttcatt gtctgctgga cccctatcca
      961 tatctacgta ataattaaag ctctggtgac aatcccggaa acaacgtttc agacagtatc
     1021 atggcacttt tgtatagccc taggatatac aaatagctgc ttaaaccctg tactgtacgc
     1081 ctttctggat gaaaacttca agcgctgttt tcgtgagttt tgtatcccca ctagcagtaa
     1141 tatcgaacag cagaatagca cacgcattcg tcagaatacc cgcgaccacc cctcgacagc
     1201 caacacggtt gaccgtacaa atcatcagtt agaaaactta gaagcggaga cggcgcctct
     1261 cccgtgatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1321 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1381 cgggtgggcc tttctgcgtt tata
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