##gff-version 3
##sequence-region BBa_M1458 1 1404
BBa_M1458	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M1458	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M1458	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M1458	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M1458	.	CDS	65	1267	.	+	0	ID=BBa_M1456;Name=BBa_M1456
BBa_M1458	.	start_codon	65	67	.	+	0	ID=annotation2173109;Name=Start;Parent=BBa_M1456
BBa_M1458	.	stop_codon	1265	1267	.	+	0	ID=annotation2173111;Name=Stop;Parent=BBa_M1456
BBa_M1458	.	CDS	68	1264	.	+	0	ID=annotation2173110;Name=Coding;Parent=BBa_M1456
BBa_M1458	.	terminator	1276	1355	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1458	.	stem_loop	1287	1330	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1458	.	engineered_region	1276	1355	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1458	.	terminator	1364	1404	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1458	.	stop_codon	1397	1397	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1458	.	stem_loop	1371	1390	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1458	.	engineered_region	1364	1404	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1458	.	polyA_site	1391	1404	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1458	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_M1458
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggatagctccgca
gcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatggg
taaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcct
ttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggc
ctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacattttta
atttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttt
tggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatg
tcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataa
acgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggag
catcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgca
tttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccg
gttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctg
gacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcac
ttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgct
gttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcga
ccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccg
tgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata