BBa_M1458
1
BBa_M1458
Opioid constitutive
2012-04-23T11:00:00Z
2015-05-08T01:13:56Z
See information for BBa_M1456
Human Opioid receptor in a complete circuit to enable constitutive transcription
false
false
_768_
0
10129
9
Not in stock
false
Added constitutive promoter and RBS with double terminator
false
Eric B Anderson
component2173140
1
BBa_M1456
component2173133
1
BBa_J23100
component2173135
1
BBa_B0030
component2173143
1
BBa_B0012
component2173141
1
BBa_B0010
annotation2173135
1
BBa_B0030
range2173135
1
44
58
annotation2173143
1
BBa_B0012
range2173143
1
1364
1404
annotation2173133
1
BBa_J23100
range2173133
1
1
35
annotation2173140
1
BBa_M1456
range2173140
1
65
1267
annotation2173141
1
BBa_B0010
range2173141
1
1276
1355
BBa_M1456
1
BBa_M1456
Mu Opioid receptor (human)
2012-04-23T11:00:00Z
2015-05-08T01:13:56Z
From aa sequence found on NCBI, attr. to Mestek, A. et al.
Backtranslated human opioid receptor (mu type). It has been found to react with prolamines like gliadins (subunits of wheat protein gluten)
false
false
_768_
0
10129
9
Not in stock
false
E. Coli stop codon TGA added in silico to the end of the coding sequence.
false
Eric B Anderson
annotation2173111
1
Stop
range2173111
1
1201
1203
annotation2173109
1
Start
range2173109
1
1
3
annotation2173110
1
Coding
range2173110
1
4
1200
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_M1458_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M1456_sequence
1
atggatagctccgcagcacctacgaacgcatccaactgcaccgatgcgttagcgtactcatcctgttcgcccgcaccaagtcccggttcatgggtaaacctgtcgcatctggatggtaacttgagcgacccatgcggtccgaatcgaacagacctgggtggtcgtgatagcctttgcccgccgacaggttcaccgtctatgataaccgcaatcacaattatggcgctatacagtattgtctgcgttgtgggcctttttggcaattttctggttatgtatgtcattgtccggtacaccaaaatgaagaccgccacgaatatttacatttttaatttagcattagcggatgccctggctacatcaaccctgccatttcaatctgtcaattatctcatggggacatggccttttggtactatcctgtgcaagatcgttattagtattgattattataacatgtttacctctattttcaccctttgcaccatgtcagttgatcgatatatagctgtatgtcatcccgtcaaagcgttagactttcggactcctcggaatgccaaaatcataaacgtgtgcaattggatactgagttcagcaatcggcttgcctgtcatgttcatggcaacgacgaaatatcgtcaagggagcatcgattgcacactaacattctcccatccgacctggtactgggaaaatctgctgaagatatgtgttttcatatttgcatttatcatgccggtgctgattatcaccgtctgctatggactgatgattttacgccttaaatcggtgcgaatgctgtccggttctaaagaaaaggatagaaatttgagaagaattacgcggatggtgttagtggttgtggctgtgttcattgtctgctggacccctatccatatctacgtaataattaaagctctggtgacaatcccggaaacaacgtttcagacagtatcatggcacttttgtatagccctaggatatacaaatagctgcttaaaccctgtactgtacgcctttctggatgaaaacttcaagcgctgttttcgtgagttttgtatccccactagcagtaatatcgaacagcagaatagcacacgcattcgtcagaatacccgcgaccacccctcgacagccaacacggttgaccgtacaaatcatcagttagaaaacttagaagcggagacggcgcctctcccgtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z