LOCUS BBa_M1515 9170 bp DNA linear 26-Dec-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1515 VERSION BBa_M1515.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 229..414 /label=HlyA misc_feature 229..231 /label=Start misc_feature 229..414 /label=BBa_K208006 misc_feature 427..430 /label=conserved RBS 423..434 /label=BBa_B0034 misc_feature 441..443 /label=Translation Start CDS 441..812 /label=Coding Sequence CDS 441..839 /label=BBa_M1521 RBS 848..861 /label=BBa_B0035 misc_feature 868..870 /label=Start Codon misc_feature 2989..2991 /label=Stop Codon CDS 868..2988 /label=HlyB CDS 868..2991 /label=BBa_M1003 RBS 3000..3013 /label=BBa_B0035 misc_feature 3020..3022 /label=Start Codon CDS 3020..4453 /label=HlyD misc_feature 4454..4456 /label=Stop Codon CDS 3020..4456 /label=BBa_M1004 misc_feature 848..4456 /label=BBa_M1528 misc_feature 4469..4472 /label=conserved RBS 4465..4476 /label=BBa_B0034 misc_feature 4485..4670 /label=HlyA misc_feature 4485..4487 /label=Start misc_feature 4485..4670 /label=BBa_K208006 misc_feature 4683..4686 /label=conserved RBS 4679..4690 /label=BBa_B0034 misc_feature 4697..4699 /label=Start CDS 4697..5416 /label=GFP Coding Region CDS 4697..5416 /label=BBa_K208000 RBS 5425..5438 /label=BBa_B0035 misc_feature 5445..5447 /label=Start Codon misc_feature 7566..7568 /label=Stop Codon CDS 5445..7565 /label=HlyB CDS 5445..7568 /label=BBa_M1003 RBS 7577..7590 /label=BBa_B0035 misc_feature 7597..7599 /label=Start Codon CDS 7597..9030 /label=HlyD misc_feature 9031..9033 /label=Stop Codon CDS 7597..9033 /label=BBa_M1004 misc_feature 5425..9033 /label=BBa_M1528 stem_loop 9053..9096 /label=stem_loop misc_feature 9042..9121 /label=BBa_B0010 terminator 9042..9121 /label=BBa_B0010 stem_loop 9137..9156 /label=T7 TE misc_feature 9163..9163 /label=stop polyA_site 9157..9170 /label=polya misc_feature 9130..9170 /label=BBa_B0012 terminator 9130..9170 /label=BBa_B0012 terminator 9042..9170 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt agcctatgga 241 agtcagggtg atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt 301 agcttcgatg ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc 361 agtgattttt catatggacg gaactcaata accctgacca catcagcata ataatactag 421 agaaagagga gaaatactag atggccttta aaacatgcgg atttagtaaa aaatggttaa 481 tcatagccgt tattgtgatg tgcttatgta cagaatgcta ttgccagtgc acaggaggtg 541 ctgattgtac cagttgcacc gctgcctgca cgggctgcgg ctcttgtccc aatgctcata 601 cgtgtacaga ttcaaaaaat tgtgttcgtg cagaaacctg tacagattcc gaaaattgcg 661 tgaaagccca tacttgtacg ggttcccgca attgcaatac cgcaatgacg tgtacaaact 721 cgaaagactg ctttgaagca aaaacctgta ccgattccac caattgttat aaggcaaccg 781 cgtgtactaa ttctaccggg tgtccaggac atcatcatca ccatcatcac catcactagt 841 actagagatt aaagaggaga atactagatg gattcctgtc acaagattga ttacggcctg 901 tatgcactgg aaatcctggc acaatatcat aacgtctccg tcaacccgga ggaaattaaa 961 catcgcttcg atactgacgg cactggcctg ggtctgacca gctggctgct ggcagcgaag 1021 tccctggagc tgaaggtaaa gcaagtgaaa aagacgattg atcgtctgaa cttcatcagc 1081 ctgccggcac tggtttggcg tgaagatggc cgtcacttca tcctgacgaa agtgtccaaa 1141 gaagcgaacc gctacctgat ctttgatctg gagcagcgca acccgcgcgt actggaacag 1201 agcgagttcg aggctctgta tcagggccac atcattctga ttgcatctcg ttcttctgtt 1261 gcaggtaaac tggcgaaatt cgacttcacc tggttcattc cggctattat caagtaccgt 1321 cgtattttca tcgaaaccct ggttgtatct gttttcctgc aactgttcgc tctgattact 1381 ccgctgttct ttcaggttgt catggacaaa gttctggttc accgtggctt ttccaccctg 1441 aacgtcatta ctgttgcgct gagcgttgtt gttgtgttcg aaatcattct gtccggtctg 1501 cgtacctaca tcttcgcgca ctctacctcc cgcatcgacg ttgaactggg tgcgaagctg 1561 ttccgtcacc tgctggcact gcctatcagc tacttcgaat ctcgtcgtgt gggcgatact 1621 gtggcccgtg ttcgtgaact ggatcagatc cgcaactttc tgaccggcca ggccctgacc 1681 agcgttctgg atctgctgtt cagctttatc ttcttcgcag taatgtggta ctacagcccg 1741 aagctgactc tggtgattct gttctctctg ccgtgttacg cagcttggtc cgtcttcatc 1801 tctccaatcc tgcgtcgccg tctggacgac aaatttagcc gtaatgcgga taatcagtcc 1861 ttcctggtcg aatctgtgac tgctatcaac actatcaaag ccatggcggt ttctccacag 1921 atgactaaca tctgggataa gcagctggca ggttacgttg ctgcgggctt caaagtgacc 1981 gttctggcaa ccattggtca acagggcatt cagctgattc agaaaaccgt gatgatcatt 2041 aacctgtggc tgggtgcgca cctggtcatc agcggtgacc tgtccattgg ccagctgatc 2101 gctttcaaca tgctggcggg tcaaattgtt gccccagtaa tccgtctggc ccagatctgg 2161 caggacttcc agcaggtcgg tatttctgtg acccgcctgg gtgacgtcct gaactctccg 2221 accgaaagct accacggtaa actggctctg ccggagatca acggcgatat tactttccgt 2281 aacatccgtt ttcgttacaa accggattcc ccggtaatcc tggataacat caatctgtct 2341 atcaaacagg gcgaagtcat cggcatcgtg ggccgtagcg gcagcggcaa gtccactctg 2401 actaaactga ttcagcgctt ttacattcca gaaaacggtc aggttctgat tgacggtcac 2461 gacctggctc tggcggaccc gaactggctg cgtcgtcagg tgggcgtagt gctgcaagat 2521 aacgtgctgc tgaaccgtag catcatcgat aacatcagcc tggctaaccc gggcatgtcc 2581 gtggaaaaag ttatctatgc tgcgaaactg gcaggcgcgc acgatttcat ctctgaactg 2641 cgtgaaggct acaacaccat cgtaggcgaa cagggcgctg gcctgtccgg cggtcaacgt 2701 cagcgtatcg ctattgcacg tgcactggtg aacaacccga aaatcctgat cttcgacgag 2761 gccacttctg ctctggatta cgaatctgaa catattatca tgcgtaatat gcacaagatc 2821 tgcaaaggtc gcaccgtaat catcatcgcc caccgtctga gcaccgtaaa gaacgccgac 2881 cgcatcatcg tgatggaaaa aggtaagatc gttgaacagg gtaaacataa agagctgctg 2941 tccgaaccgg aatctctgta ttcttatctg taccagctgc aatctgatta atactagaga 3001 ttaaagagga gaatactaga tgaaaacttg gctgatgggc ttctctgagt tcctgctgcg 3061 ttataagctg gtatggagcg aaacttggaa aatccgtaag caactggata ccccggtacg 3121 tgaaaaagac gaaaacgaat ttctgccagc acatctggaa ctgattgaga cgccggtttc 3181 tcgccgtcca cgtctggttg cttacttcat tatgggcttc ctggttattg ctgtaatcct 3241 gtctgttctg ggtcaggtgg aaatcgttgc gaccgcgaat ggtaaactga ccctgtccgg 3301 tcgttctaaa gaaatcaaac cgatcgaaaa ctccatcgtt aaagagatta tcgttaaaga 3361 aggtgaatcc gtacgcaaag gcgatgttct gctgaaactg accgcactgg gcgcagaagc 3421 ggacactctg aaaactcagt cctctctgct gcaaacccgt ctggaacaga cccgttacca 3481 aatcctgagc cgttctattg aactgaacaa actgccggaa ctgaaactgc cggatgaacc 3541 atacttccag aacgtaagcg aggaggaagt tctgcgcctg acttccctga tcaaagagca 3601 gttctctact tggcagaacc agaaatacca aaaggaactg aacctggaca aaaagcgtgc 3661 ggagcgcctg actatcctgg cccgcatcaa ccgttacgag aacctgtctc gtgttgaaaa 3721 atctcgcctg gacgacttcc gtagcctgct gcacaaacag gccatcgcaa aacacgctgt 3781 gctggaacag gaaaataaat acgtggaagc tgccaacgaa ctgcgtgtat ataaaagcca 3841 gctggagcag atcgagagcg aaatcctgtc tgcgaaggag gagtaccagc tggtgacgca 3901 gctgttcaaa aatgaaatcc tggacaaact gcgtcaaacc acggactcta tcgaactgct 3961 gaccctggaa ctggagaaaa acgaggagcg ccagcaggcc agcgtcatcc gcgctccggt 4021 atctggtaaa gttcagcaac tgaaagttca caccgaaggt ggcgttgtta ccaccgcaga 4081 aaccctgatg gttattgtgc cggaagatga caccctggaa gttaccgctc tggtgcagaa 4141 caaagatatc ggtttcatca acgtcggtca gaacgcgatc atcaaagtag aggctttccc 4201 gtacactcgt tacggctacc tggtgggcaa ggtcaaaaac atcaacctgg acgctatcga 4261 agatcagaaa ctgggcctgg tgttcaacgt gatcgttagc gtagaagaga acgatctgtc 4321 taccggtaac aaacacattc cactgtcctc cggcatggcg gttactgctg aaatcaaaac 4381 cggtatgcgt agcgtgatct cctatctgct gtctccgctg gaagaaagcg tcaccgaatc 4441 tctgcacgaa cgctaatact agagaaagag gagaaatact agagttagcc tatggaagtc 4501 agggtgatct taatccatta attaatgaaa tcagcaaaat catttcagca gcaggtagct 4561 tcgatgttaa agaggaaaga accgcagctt ctttattgca gttgtccggt aatgccagtg 4621 atttttcata tggacggaac tcaataaccc tgaccacatc agcataataa tactagagaa 4681 agaggagaaa tactagatgg ctagcaaagg agaagaactt ttcactggag ttgtcccaat 4741 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga 4801 aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg gaaaactacc 4861 tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcccgtta 4921 tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca 4981 ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg aagtcaagtt 5041 tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta aagaagatgg 5101 aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat acatcacggc 5161 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg 5221 atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct 5281 tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa 5341 gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga 5401 tgagctctac aaataatact agagattaaa gaggagaata ctagatggat tcctgtcaca 5461 agattgatta cggcctgtat gcactggaaa tcctggcaca atatcataac gtctccgtca 5521 acccggagga aattaaacat cgcttcgata ctgacggcac tggcctgggt ctgaccagct 5581 ggctgctggc agcgaagtcc ctggagctga aggtaaagca agtgaaaaag acgattgatc 5641 gtctgaactt catcagcctg ccggcactgg tttggcgtga agatggccgt cacttcatcc 5701 tgacgaaagt gtccaaagaa gcgaaccgct acctgatctt tgatctggag cagcgcaacc 5761 cgcgcgtact ggaacagagc gagttcgagg ctctgtatca gggccacatc attctgattg 5821 catctcgttc ttctgttgca ggtaaactgg cgaaattcga cttcacctgg ttcattccgg 5881 ctattatcaa gtaccgtcgt attttcatcg aaaccctggt tgtatctgtt ttcctgcaac 5941 tgttcgctct gattactccg ctgttctttc aggttgtcat ggacaaagtt ctggttcacc 6001 gtggcttttc caccctgaac gtcattactg ttgcgctgag cgttgttgtt gtgttcgaaa 6061 tcattctgtc cggtctgcgt acctacatct tcgcgcactc tacctcccgc atcgacgttg 6121 aactgggtgc gaagctgttc cgtcacctgc tggcactgcc tatcagctac ttcgaatctc 6181 gtcgtgtggg cgatactgtg gcccgtgttc gtgaactgga tcagatccgc aactttctga 6241 ccggccaggc cctgaccagc gttctggatc tgctgttcag ctttatcttc ttcgcagtaa 6301 tgtggtacta cagcccgaag ctgactctgg tgattctgtt ctctctgccg tgttacgcag 6361 cttggtccgt cttcatctct ccaatcctgc gtcgccgtct ggacgacaaa tttagccgta 6421 atgcggataa tcagtccttc ctggtcgaat ctgtgactgc tatcaacact atcaaagcca 6481 tggcggtttc tccacagatg actaacatct gggataagca gctggcaggt tacgttgctg 6541 cgggcttcaa agtgaccgtt ctggcaacca ttggtcaaca gggcattcag ctgattcaga 6601 aaaccgtgat gatcattaac ctgtggctgg gtgcgcacct ggtcatcagc ggtgacctgt 6661 ccattggcca gctgatcgct ttcaacatgc tggcgggtca aattgttgcc ccagtaatcc 6721 gtctggccca gatctggcag gacttccagc aggtcggtat ttctgtgacc cgcctgggtg 6781 acgtcctgaa ctctccgacc gaaagctacc acggtaaact ggctctgccg gagatcaacg 6841 gcgatattac tttccgtaac atccgttttc gttacaaacc ggattccccg gtaatcctgg 6901 ataacatcaa tctgtctatc aaacagggcg aagtcatcgg catcgtgggc cgtagcggca 6961 gcggcaagtc cactctgact aaactgattc agcgctttta cattccagaa aacggtcagg 7021 ttctgattga cggtcacgac ctggctctgg cggacccgaa ctggctgcgt cgtcaggtgg 7081 gcgtagtgct gcaagataac gtgctgctga accgtagcat catcgataac atcagcctgg 7141 ctaacccggg catgtccgtg gaaaaagtta tctatgctgc gaaactggca ggcgcgcacg 7201 atttcatctc tgaactgcgt gaaggctaca acaccatcgt aggcgaacag ggcgctggcc 7261 tgtccggcgg tcaacgtcag cgtatcgcta ttgcacgtgc actggtgaac aacccgaaaa 7321 tcctgatctt cgacgaggcc acttctgctc tggattacga atctgaacat attatcatgc 7381 gtaatatgca caagatctgc aaaggtcgca ccgtaatcat catcgcccac cgtctgagca 7441 ccgtaaagaa cgccgaccgc atcatcgtga tggaaaaagg taagatcgtt gaacagggta 7501 aacataaaga gctgctgtcc gaaccggaat ctctgtattc ttatctgtac cagctgcaat 7561 ctgattaata ctagagatta aagaggagaa tactagatga aaacttggct gatgggcttc 7621 tctgagttcc tgctgcgtta taagctggta tggagcgaaa cttggaaaat ccgtaagcaa 7681 ctggataccc cggtacgtga aaaagacgaa aacgaatttc tgccagcaca tctggaactg 7741 attgagacgc cggtttctcg ccgtccacgt ctggttgctt acttcattat gggcttcctg 7801 gttattgctg taatcctgtc tgttctgggt caggtggaaa tcgttgcgac cgcgaatggt 7861 aaactgaccc tgtccggtcg ttctaaagaa atcaaaccga tcgaaaactc catcgttaaa 7921 gagattatcg ttaaagaagg tgaatccgta cgcaaaggcg atgttctgct gaaactgacc 7981 gcactgggcg cagaagcgga cactctgaaa actcagtcct ctctgctgca aacccgtctg 8041 gaacagaccc gttaccaaat cctgagccgt tctattgaac tgaacaaact gccggaactg 8101 aaactgccgg atgaaccata cttccagaac gtaagcgagg aggaagttct gcgcctgact 8161 tccctgatca aagagcagtt ctctacttgg cagaaccaga aataccaaaa ggaactgaac 8221 ctggacaaaa agcgtgcgga gcgcctgact atcctggccc gcatcaaccg ttacgagaac 8281 ctgtctcgtg ttgaaaaatc tcgcctggac gacttccgta gcctgctgca caaacaggcc 8341 atcgcaaaac acgctgtgct ggaacaggaa aataaatacg tggaagctgc caacgaactg 8401 cgtgtatata aaagccagct ggagcagatc gagagcgaaa tcctgtctgc gaaggaggag 8461 taccagctgg tgacgcagct gttcaaaaat gaaatcctgg acaaactgcg tcaaaccacg 8521 gactctatcg aactgctgac cctggaactg gagaaaaacg aggagcgcca gcaggccagc 8581 gtcatccgcg ctccggtatc tggtaaagtt cagcaactga aagttcacac cgaaggtggc 8641 gttgttacca ccgcagaaac cctgatggtt attgtgccgg aagatgacac cctggaagtt 8701 accgctctgg tgcagaacaa agatatcggt ttcatcaacg tcggtcagaa cgcgatcatc 8761 aaagtagagg ctttcccgta cactcgttac ggctacctgg tgggcaaggt caaaaacatc 8821 aacctggacg ctatcgaaga tcagaaactg ggcctggtgt tcaacgtgat cgttagcgta 8881 gaagagaacg atctgtctac cggtaacaaa cacattccac tgtcctccgg catggcggtt 8941 actgctgaaa tcaaaaccgg tatgcgtagc gtgatctcct atctgctgtc tccgctggaa 9001 gaaagcgtca ccgaatctct gcacgaacgc taatactaga gccaggcatc aaataaaacg 9061 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 9121 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //