##gff-version 3
##sequence-region BBa_M1515 1 9170
BBa_M1515	.	engineered_region	848	4456	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1515	.	CDS	868	2991	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1515	.	CDS	868	2988	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1515	.	start_codon	868	870	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1515	.	stop_codon	2989	2991	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1515	.	ribosome_entry_site	848	861	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1515	.	ribosome_entry_site	3000	3013	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1515	.	CDS	3020	4456	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1515	.	CDS	3020	4453	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1515	.	start_codon	3020	3022	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1515	.	stop_codon	4454	4456	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1515	.	CDS	441	839	.	+	0	ID=BBa_M1521;Name=BBa_M1521
BBa_M1515	.	CDS	441	812	.	+	0	ID=annotation2172665;Name=Coding Sequence;Parent=BBa_M1521
BBa_M1515	.	start_codon	441	443	.	+	0	ID=annotation2172664;Name=Translation Start;Parent=BBa_M1521
BBa_M1515	.	ribosome_entry_site	4465	4476	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1515	.	conserved_region	4469	4472	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1515	.	CDS	4697	5416	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1515	.	CDS	4697	5416	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1515	.	start_codon	4697	4699	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1515	.	tag	229	414	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1515	.	start_codon	229	231	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1515	.	tag	229	414	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1515	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1515	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1515	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1515	.	ribosome_entry_site	4679	4690	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1515	.	conserved_region	4683	4686	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1515	.	tag	4485	4670	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1515	.	start_codon	4485	4487	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1515	.	tag	4485	4670	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1515	.	engineered_region	5425	9033	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1515	.	CDS	5445	7568	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1515	.	CDS	5445	7565	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1515	.	start_codon	5445	5447	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1515	.	stop_codon	7566	7568	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1515	.	ribosome_entry_site	5425	5438	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1515	.	ribosome_entry_site	7577	7590	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1515	.	CDS	7597	9033	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1515	.	CDS	7597	9030	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1515	.	start_codon	7597	7599	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1515	.	stop_codon	9031	9033	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1515	.	terminator	9042	9170	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1515	.	terminator	9130	9170	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1515	.	engineered_region	9130	9170	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1515	.	stem_loop	9137	9156	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1515	.	stop_codon	9163	9163	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1515	.	polyA_site	9157	9170	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1515	.	terminator	9042	9121	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1515	.	engineered_region	9042	9121	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1515	.	stem_loop	9053	9096	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1515	.	ribosome_entry_site	423	434	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1515	.	conserved_region	427	430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_M1515
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctat
ggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaag
aggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccct
gaccacatcagcataataatactagagaaagaggagaaatactagatggcctttaaaacatgcggatttagtaaaaaat
ggttaatcatagccgttattgtgatgtgcttatgtacagaatgctattgccagtgcacaggaggtgctgattgtaccag
ttgcaccgctgcctgcacgggctgcggctcttgtcccaatgctcatacgtgtacagattcaaaaaattgtgttcgtgca
gaaacctgtacagattccgaaaattgcgtgaaagcccatacttgtacgggttcccgcaattgcaataccgcaatgacgt
gtacaaactcgaaagactgctttgaagcaaaaacctgtaccgattccaccaattgttataaggcaaccgcgtgtactaa
ttctaccgggtgtccaggacatcatcatcaccatcatcaccatcactagtactagagattaaagaggagaatactagat
ggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccg
gaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctgg
agctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaaga
tggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccg
cgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcag
gtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggt
tgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcac
cgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgc
gtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcact
gcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaacttt
ctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcc
cgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcg
ccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatc
aaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaag
tgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctggg
tgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgcc
ccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctga
actctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttt
tcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtg
ggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctga
ttgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgct
gctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaa
ctggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgt
ccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccac
ttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatc
atcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggta
aacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagatt
aaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcga
aacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaa
ctgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcc
tgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaa
accgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaa
ctgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgtt
accaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgt
aagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaag
gaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtg
ttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacagga
aaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtct
gcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactcta
tcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaa
agttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagat
gacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaag
tagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatca
gaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactg
tcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaag
aaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggagaaatactagagttagcctatggaagtcagg
gtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaac
cgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatca
gcataataatactagagaaagaggagaaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaat
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacgga
aagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatg
gtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgt
acaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgataccctt
gttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactata
actcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattga
agatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaac
cattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaa
ctgctgctgggattacacatggcatggatgagctctacaaataatactagagattaaagaggagaatactagatggatt
cctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggagga
aattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctg
aaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggcc
gtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgt
actggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaa
ctggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtat
ctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtgg
cttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacc
tacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgccta
tcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgac
cggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaag
ctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtc
tggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagc
catggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgacc
gttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgc
acctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagt
aatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactct
ccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgtt
acaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccg
tagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgac
ggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctga
accgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggc
aggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggc
ggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctg
ctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgc
ccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacat
aaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagattaaaga
ggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaactt
ggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgat
tgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtct
gttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccga
tcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgac
cgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaa
atcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcg
aggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaact
gaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaa
aaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaata
aatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaa
ggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaa
ctgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttc
agcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacac
cctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagag
gctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaac
tgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctc
cggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagc
gtcaccgaatctctgcacgaacgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata