BBa_M1515
type: ComponentDefinition
persistent identity: BBa M1515
display id: BBa_M1515
version: 1
was generated by: iGEM to SBOL conversion
description: Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP
created: 2012-04-18T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: See subparts.
owned by: james
owned by: myers
mutable description: This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL3. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12287
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: See subparts. PstI site was removed
star: false
creator: Brian Smith
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K208010
component: BBa_B0034
component: BBa_K208006
component: BBa_M1528
component: BBa_M1521
component: BBa_K208000
component: BBa_B0015
sequence annotation: BBa_K208010
sequence annotation: BBa_K208006
sequence annotation: BBa_B0034
sequence annotation: BBa_M1521
sequence annotation: BBa_M1528
sequence annotation: BBa_K208000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1515_sequence/1

BBa_K208010
type: Component
persistent identity: component2172607
display id: component2172607
version: 1
top level: BBa_M1515
definition: BBa_K208010
access: public

BBa_B0034
type: Component
persistent identity: component2172612
display id: component2172612
version: 1
top level: BBa_M1515
definition: BBa_B0034
access: public

BBa_K208006
type: Component
persistent identity: component2172610
display id: component2172610
version: 1
top level: BBa_M1515
definition: BBa_K208006
access: public

BBa_M1528
type: Component
persistent identity: component2172628
display id: component2172628
version: 1
top level: BBa_M1515
definition: BBa_M1528
access: public

BBa_M1521
type: Component
persistent identity: component2172617
display id: component2172617
version: 1
top level: BBa_M1515
definition: BBa_M1521
access: public

BBa_K208006
type: Component
persistent identity: component2172633
display id: component2172633
version: 1
top level: BBa_M1515
definition: BBa_K208006
access: public

BBa_B0034
type: Component
persistent identity: component2172630
display id: component2172630
version: 1
top level: BBa_M1515
definition: BBa_B0034
access: public

BBa_K208000
type: Component
persistent identity: component2172638
display id: component2172638
version: 1
top level: BBa_M1515
definition: BBa_K208000
access: public

BBa_B0034
type: Component
persistent identity: component2172635
display id: component2172635
version: 1
top level: BBa_M1515
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2172656
display id: component2172656
version: 1
top level: BBa_M1515
definition: BBa_B0015
access: public

BBa_M1528
type: Component
persistent identity: component2172649
display id: component2172649
version: 1
top level: BBa_M1515
definition: BBa_M1528
access: public

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2172607
display id: annotation2172607
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172607/range2172607/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_M1515/annotation2172607/range2172607/1
type: Range
persistent identity: range2172607
display id: range2172607
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172610
display id: annotation2172610
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172610/range2172610/1
component: BBa_K208006

https://synbiohub.org/public/igem/BBa_M1515/annotation2172610/range2172610/1
type: Range
persistent identity: range2172610
display id: range2172610
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 229
end: 414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172612
display id: annotation2172612
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172612/range2172612/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1515/annotation2172612/range2172612/1
type: Range
persistent identity: range2172612
display id: range2172612
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 423
end: 434
orientation: inline

BBa_M1521
type: SequenceAnnotation
persistent identity: annotation2172617
display id: annotation2172617
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172617/range2172617/1
component: BBa_M1521

https://synbiohub.org/public/igem/BBa_M1515/annotation2172617/range2172617/1
type: Range
persistent identity: range2172617
display id: range2172617
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 441
end: 839
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172628
display id: annotation2172628
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172628/range2172628/1
component: BBa_M1528

https://synbiohub.org/public/igem/BBa_M1515/annotation2172628/range2172628/1
type: Range
persistent identity: range2172628
display id: range2172628
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 848
end: 4456
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172630
display id: annotation2172630
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172630/range2172630/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1515/annotation2172630/range2172630/1
type: Range
persistent identity: range2172630
display id: range2172630
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 4465
end: 4476
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172633
display id: annotation2172633
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172633/range2172633/1
component: BBa_K208006

https://synbiohub.org/public/igem/BBa_M1515/annotation2172633/range2172633/1
type: Range
persistent identity: range2172633
display id: range2172633
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 4485
end: 4670
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172635
display id: annotation2172635
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172635/range2172635/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1515/annotation2172635/range2172635/1
type: Range
persistent identity: range2172635
display id: range2172635
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 4679
end: 4690
orientation: inline

BBa_K208000
type: SequenceAnnotation
persistent identity: annotation2172638
display id: annotation2172638
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172638/range2172638/1
component: BBa_K208000

https://synbiohub.org/public/igem/BBa_M1515/annotation2172638/range2172638/1
type: Range
persistent identity: range2172638
display id: range2172638
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 4697
end: 5416
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172649
display id: annotation2172649
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172649/range2172649/1
component: BBa_M1528

https://synbiohub.org/public/igem/BBa_M1515/annotation2172649/range2172649/1
type: Range
persistent identity: range2172649
display id: range2172649
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 5425
end: 9033
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2172656
display id: annotation2172656
version: 1
top level: BBa_M1515
location: https://synbiohub.org/public/igem/BBa_M1515/annotation2172656/range2172656/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1515/annotation2172656/range2172656/1
type: Range
persistent identity: range2172656
display id: range2172656
version: 1
top level: BBa_M1515
direction: direction/reverse
start: 9042
end: 9170
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K208000
type: ComponentDefinition
persistent identity: BBa K208000
display id: BBa_K208000
version: 1
was generated by: iGEM to SBOL conversion
description: GFP
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: plasmid
owned by: james
owned by: myers
mutable description: GFP
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 2425
owning group id: 303
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Mutate PstI sites
star: false
creator: Elisabeth Linton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: GFP Coding Region
sequence: https://synbiohub.org/public/igem/BBa_K208000_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2062216
display id: annotation2062216
version: 1
top level: BBa_K208000
location: https://synbiohub.org/public/igem/BBa_K208000/annotation2062216/range2062216/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K208000/annotation2062216/range2062216/1
type: Range
persistent identity: range2062216
display id: range2062216
version: 1
top level: BBa_K208000
direction: direction/forward
start: 1
end: 3
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2062217
display id: annotation2062217
version: 1
top level: BBa_K208000
location: https://synbiohub.org/public/igem/BBa_K208000/annotation2062217/range2062217/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K208000/annotation2062217/range2062217/1
type: Range
persistent identity: range2062217
display id: range2062217
version: 1
top level: BBa_K208000
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K208006
type: ComponentDefinition
persistent identity: BBa K208006
display id: BBa_K208006
version: 1
was generated by: iGEM to SBOL conversion
description: HlyA Signal Peptide - Silver Fusion Compatible
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: Synthetic
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: HlyA
discontinued: false
dominant: true
experience: experience/None
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Silver Fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HlyA
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K208006_sequence/1

HlyA
type: SequenceAnnotation
persistent identity: annotation2034624
display id: annotation2034624
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
type: Range
persistent identity: range2034624
display id: range2034624
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 186
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2062223
display id: annotation2062223
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
type: Range
persistent identity: range2062223
display id: range2062223
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K208010
type: ComponentDefinition
persistent identity: BBa K208010
display id: BBa_K208010
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Promoter (BBa_R0010) and RBS (B0034)
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lac + rbs
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Elisabeth Linton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K208010_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2034626
display id: annotation2034626
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
type: Range
persistent identity: range2034626
display id: range2034626
version: 1
top level: BBa_K208010
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2034625
display id: annotation2034625
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
type: Range
persistent identity: range2034625
display id: range2034625
version: 1
top level: BBa_K208010
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_M1521
type: ComponentDefinition
persistent identity: BBa M1521
display id: BBa_M1521
version: 1
was generated by: iGEM to SBOL conversion
description: Thermal Hysteresis (Antifreeze) Protein (variant YL3)
created: 2012-04-10T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).
owned by: james
owned by: myers
mutable description: This is a thermal hysteresis (antifreeze) protein. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL3.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12287
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The codons were optimized to be used in E. coli.
star: false
creator: Brian Smith
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Translation Start
sequence annotation: Coding Sequence
sequence: https://synbiohub.org/public/igem/BBa_M1521_sequence/1

Translation Start
type: SequenceAnnotation
persistent identity: annotation2172664
display id: annotation2172664
version: 1
top level: BBa_M1521
location: https://synbiohub.org/public/igem/BBa_M1521/annotation2172664/range2172664/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1521/annotation2172664/range2172664/1
type: Range
persistent identity: range2172664
display id: range2172664
version: 1
top level: BBa_M1521
direction: direction/forward
start: 1
end: 3
orientation: inline

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2172665
display id: annotation2172665
version: 1
top level: BBa_M1521
location: https://synbiohub.org/public/igem/BBa_M1521/annotation2172665/range2172665/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1521/annotation2172665/range2172665/1
type: Range
persistent identity: range2172665
display id: range2172665
version: 1
top level: BBa_M1521
direction: direction/forward
start: 1
end: 372
orientation: inline

BBa_M1528
type: ComponentDefinition
persistent identity: BBa M1528
display id: BBa_M1528
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + HlyB + RBS + HlyD
created: 2012-04-16T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: Composite part for high levels of production of the HlyB and HlyD membrane proteins.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12290
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Sarah Allred
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1003
component: BBa_B0035
component: BBa_M1004
sequence annotation: BBa_B0035
sequence annotation: BBa_M1003
sequence annotation: BBa_M1004
sequence: https://synbiohub.org/public/igem/BBa_M1528_sequence/1

BBa_M1003
type: Component
persistent identity: component2172159
display id: component2172159
version: 1
top level: BBa_M1528
definition: BBa_M1003
access: public

BBa_B0035
type: Component
persistent identity: component2172155
display id: component2172155
version: 1
top level: BBa_M1528
definition: BBa_B0035
access: public

BBa_M1004
type: Component
persistent identity: component2172164
display id: component2172164
version: 1
top level: BBa_M1528
definition: BBa_M1004
access: public

BBa_B0035
type: Component
persistent identity: component2172160
display id: component2172160
version: 1
top level: BBa_M1528
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172155
display id: annotation2172155
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172155/range2172155/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1528/annotation2172155/range2172155/1
type: Range
persistent identity: range2172155
display id: range2172155
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172159
display id: annotation2172159
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172159/range2172159/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1528/annotation2172159/range2172159/1
type: Range
persistent identity: range2172159
display id: range2172159
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 21
end: 2144
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172160
display id: annotation2172160
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172160/range2172160/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1528/annotation2172160/range2172160/1
type: Range
persistent identity: range2172160
display id: range2172160
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 2153
end: 2166
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172164
display id: annotation2172164
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172164/range2172164/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1528/annotation2172164/range2172164/1
type: Range
persistent identity: range2172164
display id: range2172164
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 2173
end: 3609
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

https://synbiohub.org/public/igem/BBa_M1515_sequence/1
type: Sequence
persistent identity: BBa M1515 sequence
display id: BBa_M1515_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataatactagagaaagaggagaaatactagatggcctttaaaacatgcggatttagtaaaaaatggttaatcatagccgttattgtgatgtgcttatgtacagaatgctattgccagtgcacaggaggtgctgattgtaccagttgcaccgctgcctgcacgggctgcggctcttgtcccaatgctcatacgtgtacagattcaaaaaattgtgttcgtgcagaaacctgtacagattccgaaaattgcgtgaaagcccatacttgtacgggttcccgcaattgcaataccgcaatgacgtgtacaaactcgaaagactgctttgaagcaaaaacctgtaccgattccaccaattgttataaggcaaccgcgtgtactaattctaccgggtgtccaggacatcatcatcaccatcatcaccatcactagtactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208000_sequence/1
type: Sequence
persistent identity: BBa K208000 sequence
display id: BBa_K208000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208006_sequence/1
type: Sequence
persistent identity: BBa K208006 sequence
display id: BBa_K208006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208010_sequence/1
type: Sequence
persistent identity: BBa K208010 sequence
display id: BBa_K208010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1521_sequence/1
type: Sequence
persistent identity: BBa M1521 sequence
display id: BBa_M1521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcctttaaaacatgcggatttagtaaaaaatggttaatcatagccgttattgtgatgtgcttatgtacagaatgctattgccagtgcacaggaggtgctgattgtaccagttgcaccgctgcctgcacgggctgcggctcttgtcccaatgctcatacgtgtacagattcaaaaaattgtgttcgtgcagaaacctgtacagattccgaaaattgcgtgaaagcccatacttgtacgggttcccgcaattgcaataccgcaatgacgtgtacaaactcgaaagactgctttgaagcaaaaacctgtaccgattccaccaattgttataaggcaaccgcgtgtactaattctaccgggtgtccaggacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1528_sequence/1
type: Sequence
persistent identity: BBa M1528 sequence
display id: BBa_M1528_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z