LOCUS BBa_M1516 7142 bp DNA linear 18-Feb-2025 DEFINITION Antifreeze Protein (Variant YL2) ACCESSION BBa_M1516 VERSION BBa_M1516.1 FEATURES Location/Qualifiers misc_feature 80..88 /label=stop misc_feature 1..89 /label=BBa_M1520 misc_feature 96..98 /label=Start misc_feature 1140..1142 /label=Stop CDS 96..1115 /label=Fatty Acyl ACP Reductase misc_feature 1116..1139 /label=8-His Tag CDS 96..1142 /label=BBa_K654062 misc_feature 1149..1151 /label=Start misc_feature 3158..3160 /label=Stop misc_feature 3134..3157 /label=8-His Tag misc_feature 2780..2780 /label=A>C CDS 1149..3133 /label=Fatty Acyl-CoA Reductase CDS 1149..3160 /label=BBa_K654060 RBS 3377..3388 /label=BBa_B0034 promoter 3169..3368 /label=BBa_R0010 promoter 3169..3388 /label=BBa_K208010 RBS 3397..3410 /label=BBa_B0035 misc_feature 3417..3419 /label=Start Codon misc_feature 5538..5540 /label=Stop Codon CDS 3417..5537 /label=HlyB CDS 3417..5540 /label=BBa_M1003 RBS 5549..5562 /label=BBa_B0035 misc_feature 5569..5571 /label=Start Codon CDS 5569..7002 /label=HlyD misc_feature 7003..7005 /label=Stop Codon CDS 5569..7005 /label=BBa_M1004 misc_feature 3397..7005 /label=BBa_M1528 stem_loop 7025..7068 /label=stem_loop misc_feature 7014..7093 /label=BBa_B0010 terminator 7014..7093 /label=BBa_B0010 stem_loop 7109..7128 /label=T7 TE misc_feature 7135..7135 /label=stop polyA_site 7129..7142 /label=polya misc_feature 7102..7142 /label=BBa_B0012 terminator 7102..7142 /label=BBa_B0012 terminator 7014..7142 /label=BBa_B0015 ORIGIN 1 atcgacctcc ctggctctac tgcgcatctc aatctcatct gtctatctca tctgtcattc 61 tcattctgct attcttcata cttgcgtagt actagatgtt tggtcttatt ggtcatctca 121 cgagtttaga acacgcccaa gcggttgctg aagatttagg ctatcctgag tacgccaacc 181 aaggcctgga tttttggtgt tcggctcctc cccaagtggt tgataatttt caggtgaaaa 241 gtgtgacggg gcaggtgatt gaaggcaaat atgtggagtc ttgctttttg ccggaaatgt 301 taacccaacg gcggatcaaa gcggccattc gtaaaatcct caatgctatg gccctggccc 361 aaaaggtggg cttggatatt acggccctgg gaggcttttc ttcaatcgta tttgaagaat 421 ttaacctcaa gcaaaataat caagtccgca atgtggaact agattttcag cggttcacca 481 ctggtaatac ccacaccgct tatgtgatct gccgtcaggt cgagtctgga gctaaacagt 541 tgggtattga tctaagtcag gcaacggtag cggtttgtgg cgccacggga gatattggta 601 gcgccgtatg tcgttggtta gatagcaaac atcaagttaa ggaattattg ctaattgccc 661 gtaaccgcca aagattggaa aatctccaag aggaattggg tcggggcaaa attatggatt 721 tggaaacagc cctgccccag gcagatatta ttgtttgggt ggctagtatg cccaaggggg 781 tagaaattgc gggggaaatg ctgaaaaagc cctgtttgat tgtggatggg ggctatccca 841 agaatttaga caccagggtg aaagcggatg gggtgcatat tctcaagggg gggattgtag 901 aacattccct tgatattacc tgggaaatta tgaagattgt ggagatggat attccctccc 961 ggcaaatgtt cgcctgtttt gcggaggcca ttttgctaga gtttgagggc tggcgcacta 1021 atttttcctg gggccgcaac caaatttccg ttaataaaat ggaggcgatt ggtgaagctt 1081 ctgtcaagca tggcttttgc cctttagtag ctcttcatca tcaccatcat caccatcact 1141 agtactagat gaattatttc ctgacaggcg gcaccggttt tatcggtcgt tttctggttg 1201 agaaactctt ggcgcgcggc ggcaccgtgt atgttctggt tcgcgagcag tcccaggaca 1261 agctggagcg gctccgggag cgctggggtg cagacgacaa gcaagtgaag gctgtgatcg 1321 gcgacctcac cagcaaaaac cttggtattg acgcgaaaac gctgaaatca ctgaaaggaa 1381 atatcgacca cgtattccat cttgccgcgg tctacgacat gggcgcagac gaagaagccc 1441 aggccgccac caatatcgaa ggcaccaggg cggctgttca ggccgccgaa gccatgggcg 1501 ccaagcattt ccatcatgtg tcatccatcg cggcagcggg tctgttcaag ggtatcttcc 1561 gggaggatat gttcgaagaa gccgagaagc ttgatcatcc ttacctgcgc accaagcacg 1621 aatccgaaaa agttgtgcgt gaagaatgca aggttccgtt ccgcatctac cgccctggta 1681 tggtcattgg ccattcggaa accggcgaaa tggacaaggt tgacgggccc tattacttct 1741 tcaagatgat tcagaagatc cgtcatgcgt tgccccagtg ggtacccacc atcggtattg 1801 aaggtggccg gctgaacatt gtgccggtgg atttcgtggt cgatgcactg gatcacattg 1861 cccatctgga aggcgaagat ggcaactgtt tccatctggt ggactccgat ccgtataagg 1921 tgggtgagat cctcaatatt ttctgcgagg ccggccatgc cccccgcatg ggtatgcgca 1981 tcgattcccg gatgttcggt tttattccgc cgtttattcg ccagagcatc aagaatctgc 2041 ctccggtcaa gcgcattact ggtgcgcttc tggatgacat gggcattccg ccctcggtga 2101 tgtccttcat taattacccg acccgttttg atacccggga gctggagcgg gttctgaagg 2161 gcacagacat tgaggtgccg cgtctgccgt cctatgcccc ggttatctgg gactactggg 2221 agcgcaatct ggacccggac ctgttcaagg accgcaccct caagggcacg gttgaaggta 2281 aggtttgcgt ggtcaccggc gcgacctcgg gtattggcct ggcaacggca gagaagctgg 2341 cagaggccgg tgccattctg gtcattggtg cgcgcaccaa ggaaactctg gatgaagtgg 2401 cggccagtct ggaggccaag ggtggcaacg tgcatgcgta ccagtgcgac ttttcggaca 2461 tggacgactg cgaccgcttt gtgaagacgg tgctggataa tcacggccac gtggatgtac 2521 tggtgaataa cgcgggtcgc tccatccgcc gctcgctggc gttgtctttt gaccggttcc 2581 acgattttga gcggaccatg cagctgaact actttggctc cgttcggctg atcatgggct 2641 ttgcgccagc catgctggag cgtcgccgcg ggcacgtggt gaatatttct tccatcgggg 2701 tacttaccaa cgctccgcgt ttctcggcct atgtctcctc gaaatccgca ctggacgcgt 2761 tcagccgctg tgccgctgcc gaatggtcgg atcgcaacgt gaccttcacc accatcaaca 2821 tgccgttggt gaaaacgccg atgatcgcgc ccaccaagat ctacgattcc gtgccgacgc 2881 tgacgccgga tgaagccgcc cagatggtgg cggatgcgat tgtgtaccgg cccaagcgca 2941 ttgccacccg tcttggcgtg ttcgcgcagg ttctgcatgc gctggcaccg aagatgggtg 3001 agatcattat gaacactggc taccggatgt tcccggattc tccagcagcc gctggcagca 3061 agtccggcga aaagccgaaa gtctctaccg agcaggtggc ctttgcggcg attatgcggg 3121 ggatatactg gagcatcatc accatcatca ccatcactaa tactagagca atacgcaaac 3181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 3241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 3301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 3361 ttcacacata ctagagaaag aggagaaata ctagagatta aagaggagaa tactagatgg 3421 attcctgtca caagattgat tacggcctgt atgcactgga aatcctggca caatatcata 3481 acgtctccgt caacccggag gaaattaaac atcgcttcga tactgacggc actggcctgg 3541 gtctgaccag ctggctgctg gcagcgaagt ccctggagct gaaggtaaag caagtgaaaa 3601 agacgattga tcgtctgaac ttcatcagcc tgccggcact ggtttggcgt gaagatggcc 3661 gtcacttcat cctgacgaaa gtgtccaaag aagcgaaccg ctacctgatc tttgatctgg 3721 agcagcgcaa cccgcgcgta ctggaacaga gcgagttcga ggctctgtat cagggccaca 3781 tcattctgat tgcatctcgt tcttctgttg caggtaaact ggcgaaattc gacttcacct 3841 ggttcattcc ggctattatc aagtaccgtc gtattttcat cgaaaccctg gttgtatctg 3901 ttttcctgca actgttcgct ctgattactc cgctgttctt tcaggttgtc atggacaaag 3961 ttctggttca ccgtggcttt tccaccctga acgtcattac tgttgcgctg agcgttgttg 4021 ttgtgttcga aatcattctg tccggtctgc gtacctacat cttcgcgcac tctacctccc 4081 gcatcgacgt tgaactgggt gcgaagctgt tccgtcacct gctggcactg cctatcagct 4141 acttcgaatc tcgtcgtgtg ggcgatactg tggcccgtgt tcgtgaactg gatcagatcc 4201 gcaactttct gaccggccag gccctgacca gcgttctgga tctgctgttc agctttatct 4261 tcttcgcagt aatgtggtac tacagcccga agctgactct ggtgattctg ttctctctgc 4321 cgtgttacgc agcttggtcc gtcttcatct ctccaatcct gcgtcgccgt ctggacgaca 4381 aatttagccg taatgcggat aatcagtcct tcctggtcga atctgtgact gctatcaaca 4441 ctatcaaagc catggcggtt tctccacaga tgactaacat ctgggataag cagctggcag 4501 gttacgttgc tgcgggcttc aaagtgaccg ttctggcaac cattggtcaa cagggcattc 4561 agctgattca gaaaaccgtg atgatcatta acctgtggct gggtgcgcac ctggtcatca 4621 gcggtgacct gtccattggc cagctgatcg ctttcaacat gctggcgggt caaattgttg 4681 ccccagtaat ccgtctggcc cagatctggc aggacttcca gcaggtcggt atttctgtga 4741 cccgcctggg tgacgtcctg aactctccga ccgaaagcta ccacggtaaa ctggctctgc 4801 cggagatcaa cggcgatatt actttccgta acatccgttt tcgttacaaa ccggattccc 4861 cggtaatcct ggataacatc aatctgtcta tcaaacaggg cgaagtcatc ggcatcgtgg 4921 gccgtagcgg cagcggcaag tccactctga ctaaactgat tcagcgcttt tacattccag 4981 aaaacggtca ggttctgatt gacggtcacg acctggctct ggcggacccg aactggctgc 5041 gtcgtcaggt gggcgtagtg ctgcaagata acgtgctgct gaaccgtagc atcatcgata 5101 acatcagcct ggctaacccg ggcatgtccg tggaaaaagt tatctatgct gcgaaactgg 5161 caggcgcgca cgatttcatc tctgaactgc gtgaaggcta caacaccatc gtaggcgaac 5221 agggcgctgg cctgtccggc ggtcaacgtc agcgtatcgc tattgcacgt gcactggtga 5281 acaacccgaa aatcctgatc ttcgacgagg ccacttctgc tctggattac gaatctgaac 5341 atattatcat gcgtaatatg cacaagatct gcaaaggtcg caccgtaatc atcatcgccc 5401 accgtctgag caccgtaaag aacgccgacc gcatcatcgt gatggaaaaa ggtaagatcg 5461 ttgaacaggg taaacataaa gagctgctgt ccgaaccgga atctctgtat tcttatctgt 5521 accagctgca atctgattaa tactagagat taaagaggag aatactagat gaaaacttgg 5581 ctgatgggct tctctgagtt cctgctgcgt tataagctgg tatggagcga aacttggaaa 5641 atccgtaagc aactggatac cccggtacgt gaaaaagacg aaaacgaatt tctgccagca 5701 catctggaac tgattgagac gccggtttct cgccgtccac gtctggttgc ttacttcatt 5761 atgggcttcc tggttattgc tgtaatcctg tctgttctgg gtcaggtgga aatcgttgcg 5821 accgcgaatg gtaaactgac cctgtccggt cgttctaaag aaatcaaacc gatcgaaaac 5881 tccatcgtta aagagattat cgttaaagaa ggtgaatccg tacgcaaagg cgatgttctg 5941 ctgaaactga ccgcactggg cgcagaagcg gacactctga aaactcagtc ctctctgctg 6001 caaacccgtc tggaacagac ccgttaccaa atcctgagcc gttctattga actgaacaaa 6061 ctgccggaac tgaaactgcc ggatgaacca tacttccaga acgtaagcga ggaggaagtt 6121 ctgcgcctga cttccctgat caaagagcag ttctctactt ggcagaacca gaaataccaa 6181 aaggaactga acctggacaa aaagcgtgcg gagcgcctga ctatcctggc ccgcatcaac 6241 cgttacgaga acctgtctcg tgttgaaaaa tctcgcctgg acgacttccg tagcctgctg 6301 cacaaacagg ccatcgcaaa acacgctgtg ctggaacagg aaaataaata cgtggaagct 6361 gccaacgaac tgcgtgtata taaaagccag ctggagcaga tcgagagcga aatcctgtct 6421 gcgaaggagg agtaccagct ggtgacgcag ctgttcaaaa atgaaatcct ggacaaactg 6481 cgtcaaacca cggactctat cgaactgctg accctggaac tggagaaaaa cgaggagcgc 6541 cagcaggcca gcgtcatccg cgctccggta tctggtaaag ttcagcaact gaaagttcac 6601 accgaaggtg gcgttgttac caccgcagaa accctgatgg ttattgtgcc ggaagatgac 6661 accctggaag ttaccgctct ggtgcagaac aaagatatcg gtttcatcaa cgtcggtcag 6721 aacgcgatca tcaaagtaga ggctttcccg tacactcgtt acggctacct ggtgggcaag 6781 gtcaaaaaca tcaacctgga cgctatcgaa gatcagaaac tgggcctggt gttcaacgtg 6841 atcgttagcg tagaagagaa cgatctgtct accggtaaca aacacattcc actgtcctcc 6901 ggcatggcgg ttactgctga aatcaaaacc ggtatgcgta gcgtgatctc ctatctgctg 6961 tctccgctgg aagaaagcgt caccgaatct ctgcacgaac gctaatacta gagccaggca 7021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 7081 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 7141 ta //