##gff-version 3
##sequence-region BBa_M1516 1 7142
BBa_M1516	.	engineered_region	3397	7005	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1516	.	ribosome_entry_site	5549	5562	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1516	.	ribosome_entry_site	3397	3410	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1516	.	CDS	5569	7005	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1516	.	CDS	5569	7002	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1516	.	start_codon	5569	5571	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1516	.	stop_codon	7003	7005	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1516	.	CDS	3417	5540	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1516	.	start_codon	3417	3419	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1516	.	stop_codon	5538	5540	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1516	.	CDS	3417	5537	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1516	.	terminator	7014	7142	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1516	.	terminator	7014	7093	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1516	.	stem_loop	7025	7068	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1516	.	engineered_region	7014	7093	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1516	.	terminator	7102	7142	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1516	.	engineered_region	7102	7142	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1516	.	stop_codon	7135	7135	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1516	.	stem_loop	7109	7128	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1516	.	polyA_site	7129	7142	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1516	.	CDS	1149	3160	.	+	0	ID=BBa_K654060;Name=BBa_K654060
BBa_M1516	.	stop_codon	3158	3160	.	+	0	ID=annotation2141921;Name=Stop;Parent=BBa_K654060
BBa_M1516	.	sequence_alteration	2780	2780	.	+	0	ID=annotation2141923;Name=A>C;Parent=BBa_K654060
BBa_M1516	.	tag	3134	3157	.	+	0	ID=annotation2141922;Name=8-His Tag;Parent=BBa_K654060
BBa_M1516	.	start_codon	1149	1151	.	+	0	ID=annotation2141920;Name=Start;Parent=BBa_K654060
BBa_M1516	.	CDS	1149	3133	.	+	0	ID=annotation2141924;Name=Fatty Acyl-CoA Reductase;Parent=BBa_K654060
BBa_M1516	.	CDS	96	1142	.	+	0	ID=BBa_K654062;Name=BBa_K654062
BBa_M1516	.	CDS	96	1115	.	+	0	ID=annotation2141940;Name=Fatty Acyl ACP Reductase;Parent=BBa_K654062
BBa_M1516	.	stop_codon	1140	1142	.	+	0	ID=annotation2141930;Name=Stop;Parent=BBa_K654062
BBa_M1516	.	tag	1116	1139	.	+	0	ID=annotation2141941;Name=8-His Tag;Parent=BBa_K654062
BBa_M1516	.	start_codon	96	98	.	+	0	ID=annotation2141929;Name=Start;Parent=BBa_K654062
BBa_M1516	.	sequence_feature	1	89	.	+	0	ID=BBa_M1520;Name=BBa_M1520
BBa_M1516	.	stop_codon	80	88	.	+	0	ID=annotation2172168;Name=stop;Parent=BBa_M1520
BBa_M1516	.	promoter	3169	3388	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1516	.	ribosome_entry_site	3377	3388	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1516	.	promoter	3169	3368	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
>BBa_M1516
atcgacctccctggctctactgcgcatctcaatctcatctgtctatctcatctgtcattctcattctgctattcttcat
acttgcgtagtactagatgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagattta
ggctatcctgagtacgccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtga
aaagtgtgacggggcaggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggat
caaagcggccattcgtaaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggc
ttttcttcaatcgtatttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggt
tcaccactggtaatacccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatct
aagtcaggcaacggtagcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacat
caagttaaggaattattgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaa
ttatggatttggaaacagccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgc
gggggaaatgctgaaaaagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggat
ggggtgcatattctcaagggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatgg
atattccctcccggcaaatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttc
ctggggccgcaaccaaatttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgcccttta
gtagctcttcatcatcaccatcatcaccatcactagtactagatgaattatttcctgacaggcggcaccggttttatcg
gtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagct
ggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaacctt
ggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgg
gcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgc
caagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaa
gccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgt
tccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattactt
cttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaac
attgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatc
tggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtat
gcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgc
attactggtgcgcttctggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgata
cccgggagctggagcgggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctggga
ctactgggagcgcaatctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtg
gtcaccggcgcgacctcgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtg
cgcgcaccaaggaaactctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcga
cttttcggacatggacgactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaat
aacgcgggtcgctccatccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctga
actactttggctccgttcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatat
ttcttccatcggggtacttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagc
cgctgtgccgctgccgaatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatga
tcgcgcccaccaagatctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgt
gtaccggcccaagcgcattgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgag
atcattatgaacactggctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaag
tctctaccgagcaggtggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcatcaccatcactaa
tactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgc
ttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaga
ttaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatat
cataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggc
tgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgcc
ggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatcttt
gatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattg
catctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcg
tattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtc
atggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcg
aaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagct
gttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaa
ctggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcg
cagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtctt
catctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatct
gtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggtt
acgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgat
gatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatg
ctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtga
cccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatat
tactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacag
ggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattc
cagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgt
agtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaa
aaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtag
gcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaat
cctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgc
aaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaag
gtaagatcgttgaacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgca
atctgattaatactagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcg
ttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaa
tttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggct
tcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtc
cggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgc
aaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaa
cccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccgga
tgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttgg
cagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaacc
gttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaa
acacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcag
atcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaac
tgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcat
ccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctg
atggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcg
gtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaa
cctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctacc
ggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcct
atctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata