BBa_M1516
type: ComponentDefinition
persistent identity: BBa M1516
display id: BBa_M1516
version: 1
was generated by: iGEM to SBOL conversion
description: Antifreeze Protein (Variant YL2)
created: 2012-04-16T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: There are 4 different variants of antifreeze proteins. This is YL2, the second variant.
owned by: james
owned by: myers
mutable description: There are 4 different variants of antifreeze proteins. This is YL2, the second variant.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: There are 4 different variants of antifreeze proteins. This is YL2, the second variant.
star: false
creator: sarah guzman
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K654060
component: BBa_B0015
component: BBa_M1528
component: BBa_K654062
component: BBa_M1520
component: BBa_K208010
sequence annotation: BBa_M1520
sequence annotation: BBa_K654062
sequence annotation: BBa_K654060
sequence annotation: BBa_K208010
sequence annotation: BBa_M1528
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M1516_sequence/1

BBa_K654060
type: Component
persistent identity: component2172363
display id: component2172363
version: 1
top level: BBa_M1516
definition: BBa_K654060
access: public

BBa_B0015
type: Component
persistent identity: component2172384
display id: component2172384
version: 1
top level: BBa_M1516
definition: BBa_B0015
access: public

BBa_M1528
type: Component
persistent identity: component2172377
display id: component2172377
version: 1
top level: BBa_M1516
definition: BBa_M1528
access: public

BBa_K654062
type: Component
persistent identity: component2172357
display id: component2172357
version: 1
top level: BBa_M1516
definition: BBa_K654062
access: public

BBa_M1520
type: Component
persistent identity: component2172352
display id: component2172352
version: 1
top level: BBa_M1516
definition: BBa_M1520
access: public

BBa_K208010
type: Component
persistent identity: component2172366
display id: component2172366
version: 1
top level: BBa_M1516
definition: BBa_K208010
access: public

BBa_M1520
type: SequenceAnnotation
persistent identity: annotation2172352
display id: annotation2172352
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172352/range2172352/1
component: BBa_M1520

https://synbiohub.org/public/igem/BBa_M1516/annotation2172352/range2172352/1
type: Range
persistent identity: range2172352
display id: range2172352
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_K654062
type: SequenceAnnotation
persistent identity: annotation2172357
display id: annotation2172357
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172357/range2172357/1
component: BBa_K654062

https://synbiohub.org/public/igem/BBa_M1516/annotation2172357/range2172357/1
type: Range
persistent identity: range2172357
display id: range2172357
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 96
end: 1142
orientation: inline

BBa_K654060
type: SequenceAnnotation
persistent identity: annotation2172363
display id: annotation2172363
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172363/range2172363/1
component: BBa_K654060

https://synbiohub.org/public/igem/BBa_M1516/annotation2172363/range2172363/1
type: Range
persistent identity: range2172363
display id: range2172363
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 1149
end: 3160
orientation: inline

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2172366
display id: annotation2172366
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172366/range2172366/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_M1516/annotation2172366/range2172366/1
type: Range
persistent identity: range2172366
display id: range2172366
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 3169
end: 3388
orientation: inline

BBa_M1528
type: SequenceAnnotation
persistent identity: annotation2172377
display id: annotation2172377
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172377/range2172377/1
component: BBa_M1528

https://synbiohub.org/public/igem/BBa_M1516/annotation2172377/range2172377/1
type: Range
persistent identity: range2172377
display id: range2172377
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 3397
end: 7005
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2172384
display id: annotation2172384
version: 1
top level: BBa_M1516
location: https://synbiohub.org/public/igem/BBa_M1516/annotation2172384/range2172384/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M1516/annotation2172384/range2172384/1
type: Range
persistent identity: range2172384
display id: range2172384
version: 1
top level: BBa_M1516
direction: direction/reverse
start: 7014
end: 7142
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K208010
type: ComponentDefinition
persistent identity: BBa K208010
display id: BBa_K208010
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Promoter (BBa_R0010) and RBS (B0034)
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lac + rbs
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Elisabeth Linton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K208010_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2034626
display id: annotation2034626
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
type: Range
persistent identity: range2034626
display id: range2034626
version: 1
top level: BBa_K208010
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2034625
display id: annotation2034625
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
type: Range
persistent identity: range2034625
display id: range2034625
version: 1
top level: BBa_K208010
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_M1520
type: ComponentDefinition
persistent identity: BBa M1520
display id: BBa_M1520
version: 1
was generated by: iGEM to SBOL conversion
description: Antifreeze Protein (Variant YL2)
created: 2012-04-16T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: YL2 is a antifreeze protein. It is the second of a total of 4th.
owned by: james
owned by: myers
mutable description: YL2 is a antifreeze protein. It is the second of a total of 4th.
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: YL2 is a antifreeze protein. It is the second of a total of 4th.
star: false
creator: sarah guzman
type: DnaRegion
role: partType/Temporary
role: SO:0000110
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M1520_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2172168
display id: annotation2172168
version: 1
top level: BBa_M1520
location: https://synbiohub.org/public/igem/BBa_M1520/annotation2172168/range2172168/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1520/annotation2172168/range2172168/1
type: Range
persistent identity: range2172168
display id: range2172168
version: 1
top level: BBa_M1520
direction: direction/forward
start: 80
end: 88
orientation: inline

BBa_M1528
type: ComponentDefinition
persistent identity: BBa M1528
display id: BBa_M1528
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + HlyB + RBS + HlyD
created: 2012-04-16T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: Composite part for high levels of production of the HlyB and HlyD membrane proteins.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 12290
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Sarah Allred
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M1003
component: BBa_B0035
component: BBa_M1004
sequence annotation: BBa_B0035
sequence annotation: BBa_M1003
sequence annotation: BBa_M1004
sequence: https://synbiohub.org/public/igem/BBa_M1528_sequence/1

BBa_M1003
type: Component
persistent identity: component2172159
display id: component2172159
version: 1
top level: BBa_M1528
definition: BBa_M1003
access: public

BBa_B0035
type: Component
persistent identity: component2172155
display id: component2172155
version: 1
top level: BBa_M1528
definition: BBa_B0035
access: public

BBa_M1004
type: Component
persistent identity: component2172164
display id: component2172164
version: 1
top level: BBa_M1528
definition: BBa_M1004
access: public

BBa_B0035
type: Component
persistent identity: component2172160
display id: component2172160
version: 1
top level: BBa_M1528
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172155
display id: annotation2172155
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172155/range2172155/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1528/annotation2172155/range2172155/1
type: Range
persistent identity: range2172155
display id: range2172155
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2172159
display id: annotation2172159
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172159/range2172159/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1528/annotation2172159/range2172159/1
type: Range
persistent identity: range2172159
display id: range2172159
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 21
end: 2144
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2172160
display id: annotation2172160
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172160/range2172160/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1528/annotation2172160/range2172160/1
type: Range
persistent identity: range2172160
display id: range2172160
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 2153
end: 2166
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2172164
display id: annotation2172164
version: 1
top level: BBa_M1528
location: https://synbiohub.org/public/igem/BBa_M1528/annotation2172164/range2172164/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1528/annotation2172164/range2172164/1
type: Range
persistent identity: range2172164
display id: range2172164
version: 1
top level: BBa_M1528
direction: direction/reverse
start: 2173
end: 3609
orientation: inline

BBa_K654060
type: ComponentDefinition
persistent identity: BBa K654060
display id: BBa_K654060
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl-CoA Reductase with 8-His Tag
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: Marinobacter aquaeolei genomic DNA
owned by: james
owned by: myers
mutable description: Maqu_2507 Fatty Acyl CoA Reductase (From Marinobacter aquaeolei) EC 1.2.1.50
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: mutagenesis removal of PstI site, without altering amino acid sequence
star: false
creator: Cody Tramp
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Fatty Acyl-CoA Reductase
sequence annotation: Start
sequence annotation: A>C
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654060_sequence/1

Fatty Acyl-CoA Reductase
type: SequenceAnnotation
persistent identity: annotation2141924
display id: annotation2141924
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141924/range2141924/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K654060/annotation2141924/range2141924/1
type: Range
persistent identity: range2141924
display id: range2141924
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1
end: 1985
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2141920
display id: annotation2141920
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141920/range2141920/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K654060/annotation2141920/range2141920/1
type: Range
persistent identity: range2141920
display id: range2141920
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1
end: 3
orientation: inline

A>C
type: SequenceAnnotation
persistent identity: annotation2141923
display id: annotation2141923
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141923/range2141923/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K654060/annotation2141923/range2141923/1
type: Range
persistent identity: range2141923
display id: range2141923
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1632
end: 1632
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2141922
display id: annotation2141922
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141922/range2141922/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K654060/annotation2141922/range2141922/1
type: Range
persistent identity: range2141922
display id: range2141922
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1986
end: 2009
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2141921
display id: annotation2141921
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141921/range2141921/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K654060/annotation2141921/range2141921/1
type: Range
persistent identity: range2141921
display id: range2141921
version: 1
top level: BBa_K654060
direction: direction/forward
start: 2010
end: 2012
orientation: inline

BBa_K654062
type: ComponentDefinition
persistent identity: BBa K654062
display id: BBa_K654062
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl ACP Reductase with 8-His Tag
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: Synechocystis sp. PCC 6803 genomic DNA
owned by: james
owned by: myers
mutable description: sll0209 Fatty Acyl ACP Reductase (from Synechocystis sp. PCC 6803) EC 1.3.1.10
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Fatty Acyl ACP Reductase
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654062_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2141929
display id: annotation2141929
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141929/range2141929/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K654062/annotation2141929/range2141929/1
type: Range
persistent identity: range2141929
display id: range2141929
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1
end: 3
orientation: inline

Fatty Acyl ACP Reductase
type: SequenceAnnotation
persistent identity: annotation2141940
display id: annotation2141940
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141940/range2141940/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K654062/annotation2141940/range2141940/1
type: Range
persistent identity: range2141940
display id: range2141940
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1
end: 1020
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2141941
display id: annotation2141941
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141941/range2141941/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K654062/annotation2141941/range2141941/1
type: Range
persistent identity: range2141941
display id: range2141941
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1021
end: 1044
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2141930
display id: annotation2141930
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141930/range2141930/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K654062/annotation2141930/range2141930/1
type: Range
persistent identity: range2141930
display id: range2141930
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1045
end: 1047
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

https://synbiohub.org/public/igem/BBa_M1516_sequence/1
type: Sequence
persistent identity: BBa M1516 sequence
display id: BBa_M1516_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcgacctccctggctctactgcgcatctcaatctcatctgtctatctcatctgtcattctcattctgctattcttcatacttgcgtagtactagatgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagatttaggctatcctgagtacgccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtgaaaagtgtgacggggcaggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggatcaaagcggccattcgtaaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggcttttcttcaatcgtatttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggttcaccactggtaatacccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatctaagtcaggcaacggtagcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacatcaagttaaggaattattgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaattatggatttggaaacagccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgcgggggaaatgctgaaaaagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggatggggtgcatattctcaagggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatggatattccctcccggcaaatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttcctggggccgcaaccaaatttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgccctttagtagctcttcatcatcaccatcatcaccatcactagtactagatgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208010_sequence/1
type: Sequence
persistent identity: BBa K208010 sequence
display id: BBa_K208010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1520_sequence/1
type: Sequence
persistent identity: BBa M1520 sequence
display id: BBa_M1520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcgacctccctggctctactgcgcatctcaatctcatctgtctatctcatctgtcattctcattctgctattcttcatacttgcgtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1528_sequence/1
type: Sequence
persistent identity: BBa M1528 sequence
display id: BBa_M1528_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654060_sequence/1
type: Sequence
persistent identity: BBa K654060 sequence
display id: BBa_K654060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttctggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgatacccgggagctggagcgggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctgggactactgggagcgcaatctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtggtcaccggcgcgacctcgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtgcgcgcaccaaggaaactctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcgacttttcggacatggacgactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaataacgcgggtcgctccatccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctgaactactttggctccgttcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatatttcttccatcggggtacttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagccgctgtgccgctgccgaatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatgatcgcgcccaccaagatctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgtgtaccggcccaagcgcattgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgagatcattatgaacactggctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaagtctctaccgagcaggtggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654062_sequence/1
type: Sequence
persistent identity: BBa K654062 sequence
display id: BBa_K654062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagatttaggctatcctgagtacgccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtgaaaagtgtgacggggcaggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggatcaaagcggccattcgtaaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggcttttcttcaatcgtatttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggttcaccactggtaatacccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatctaagtcaggcaacggtagcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacatcaagttaaggaattattgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaattatggatttggaaacagccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgcgggggaaatgctgaaaaagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggatggggtgcatattctcaagggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatggatattccctcccggcaaatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttcctggggccgcaaccaaatttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgccctttagtagctcttcatcatcaccatcatcaccatcactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z