LOCUS BBa_M1517 3388 bp DNA linear 26-Nov-2024 DEFINITION Antifreeze Protein (Variant YL2) ACCESSION BBa_M1517 VERSION BBa_M1517.1 FEATURES Location/Qualifiers misc_feature 80..88 /label=stop misc_feature 1..89 /label=BBa_M1520 misc_feature 96..98 /label=Start misc_feature 1140..1142 /label=Stop CDS 96..1115 /label=Fatty Acyl ACP Reductase misc_feature 1116..1139 /label=8-His Tag CDS 96..1142 /label=BBa_K654062 misc_feature 1149..1151 /label=Start misc_feature 3158..3160 /label=Stop misc_feature 3134..3157 /label=8-His Tag misc_feature 2780..2780 /label=A>C CDS 1149..3133 /label=Fatty Acyl-CoA Reductase CDS 1149..3160 /label=BBa_K654060 RBS 3377..3388 /label=BBa_B0034 promoter 3169..3368 /label=BBa_R0010 promoter 3169..3388 /label=BBa_K208010 ORIGIN 1 atcgacctcc ctggctctac tgcgcatctc aatctcatct gtctatctca tctgtcattc 61 tcattctgct attcttcata cttgcgtagt actagatgtt tggtcttatt ggtcatctca 121 cgagtttaga acacgcccaa gcggttgctg aagatttagg ctatcctgag tacgccaacc 181 aaggcctgga tttttggtgt tcggctcctc cccaagtggt tgataatttt caggtgaaaa 241 gtgtgacggg gcaggtgatt gaaggcaaat atgtggagtc ttgctttttg ccggaaatgt 301 taacccaacg gcggatcaaa gcggccattc gtaaaatcct caatgctatg gccctggccc 361 aaaaggtggg cttggatatt acggccctgg gaggcttttc ttcaatcgta tttgaagaat 421 ttaacctcaa gcaaaataat caagtccgca atgtggaact agattttcag cggttcacca 481 ctggtaatac ccacaccgct tatgtgatct gccgtcaggt cgagtctgga gctaaacagt 541 tgggtattga tctaagtcag gcaacggtag cggtttgtgg cgccacggga gatattggta 601 gcgccgtatg tcgttggtta gatagcaaac atcaagttaa ggaattattg ctaattgccc 661 gtaaccgcca aagattggaa aatctccaag aggaattggg tcggggcaaa attatggatt 721 tggaaacagc cctgccccag gcagatatta ttgtttgggt ggctagtatg cccaaggggg 781 tagaaattgc gggggaaatg ctgaaaaagc cctgtttgat tgtggatggg ggctatccca 841 agaatttaga caccagggtg aaagcggatg gggtgcatat tctcaagggg gggattgtag 901 aacattccct tgatattacc tgggaaatta tgaagattgt ggagatggat attccctccc 961 ggcaaatgtt cgcctgtttt gcggaggcca ttttgctaga gtttgagggc tggcgcacta 1021 atttttcctg gggccgcaac caaatttccg ttaataaaat ggaggcgatt ggtgaagctt 1081 ctgtcaagca tggcttttgc cctttagtag ctcttcatca tcaccatcat caccatcact 1141 agtactagat gaattatttc ctgacaggcg gcaccggttt tatcggtcgt tttctggttg 1201 agaaactctt ggcgcgcggc ggcaccgtgt atgttctggt tcgcgagcag tcccaggaca 1261 agctggagcg gctccgggag cgctggggtg cagacgacaa gcaagtgaag gctgtgatcg 1321 gcgacctcac cagcaaaaac cttggtattg acgcgaaaac gctgaaatca ctgaaaggaa 1381 atatcgacca cgtattccat cttgccgcgg tctacgacat gggcgcagac gaagaagccc 1441 aggccgccac caatatcgaa ggcaccaggg cggctgttca ggccgccgaa gccatgggcg 1501 ccaagcattt ccatcatgtg tcatccatcg cggcagcggg tctgttcaag ggtatcttcc 1561 gggaggatat gttcgaagaa gccgagaagc ttgatcatcc ttacctgcgc accaagcacg 1621 aatccgaaaa agttgtgcgt gaagaatgca aggttccgtt ccgcatctac cgccctggta 1681 tggtcattgg ccattcggaa accggcgaaa tggacaaggt tgacgggccc tattacttct 1741 tcaagatgat tcagaagatc cgtcatgcgt tgccccagtg ggtacccacc atcggtattg 1801 aaggtggccg gctgaacatt gtgccggtgg atttcgtggt cgatgcactg gatcacattg 1861 cccatctgga aggcgaagat ggcaactgtt tccatctggt ggactccgat ccgtataagg 1921 tgggtgagat cctcaatatt ttctgcgagg ccggccatgc cccccgcatg ggtatgcgca 1981 tcgattcccg gatgttcggt tttattccgc cgtttattcg ccagagcatc aagaatctgc 2041 ctccggtcaa gcgcattact ggtgcgcttc tggatgacat gggcattccg ccctcggtga 2101 tgtccttcat taattacccg acccgttttg atacccggga gctggagcgg gttctgaagg 2161 gcacagacat tgaggtgccg cgtctgccgt cctatgcccc ggttatctgg gactactggg 2221 agcgcaatct ggacccggac ctgttcaagg accgcaccct caagggcacg gttgaaggta 2281 aggtttgcgt ggtcaccggc gcgacctcgg gtattggcct ggcaacggca gagaagctgg 2341 cagaggccgg tgccattctg gtcattggtg cgcgcaccaa ggaaactctg gatgaagtgg 2401 cggccagtct ggaggccaag ggtggcaacg tgcatgcgta ccagtgcgac ttttcggaca 2461 tggacgactg cgaccgcttt gtgaagacgg tgctggataa tcacggccac gtggatgtac 2521 tggtgaataa cgcgggtcgc tccatccgcc gctcgctggc gttgtctttt gaccggttcc 2581 acgattttga gcggaccatg cagctgaact actttggctc cgttcggctg atcatgggct 2641 ttgcgccagc catgctggag cgtcgccgcg ggcacgtggt gaatatttct tccatcgggg 2701 tacttaccaa cgctccgcgt ttctcggcct atgtctcctc gaaatccgca ctggacgcgt 2761 tcagccgctg tgccgctgcc gaatggtcgg atcgcaacgt gaccttcacc accatcaaca 2821 tgccgttggt gaaaacgccg atgatcgcgc ccaccaagat ctacgattcc gtgccgacgc 2881 tgacgccgga tgaagccgcc cagatggtgg cggatgcgat tgtgtaccgg cccaagcgca 2941 ttgccacccg tcttggcgtg ttcgcgcagg ttctgcatgc gctggcaccg aagatgggtg 3001 agatcattat gaacactggc taccggatgt tcccggattc tccagcagcc gctggcagca 3061 agtccggcga aaagccgaaa gtctctaccg agcaggtggc ctttgcggcg attatgcggg 3121 ggatatactg gagcatcatc accatcatca ccatcactaa tactagagca atacgcaaac 3181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 3241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 3301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 3361 ttcacacata ctagagaaag aggagaaa //