LOCUS BBa_M1518 6792 bp DNA linear 22-Nov-2024 DEFINITION Antifreeze Protein (Variant YL2) ACCESSION BBa_M1518 VERSION BBa_M1518.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=Start misc_feature 1045..1047 /label=Stop CDS 1..1020 /label=Fatty Acyl ACP Reductase misc_feature 1021..1044 /label=8-His Tag CDS 1..1047 /label=BBa_K654062 misc_feature 1054..1056 /label=Start misc_feature 3063..3065 /label=Stop misc_feature 3039..3062 /label=8-His Tag misc_feature 2685..2685 /label=A>C CDS 1054..3038 /label=Fatty Acyl-CoA Reductase CDS 1054..3065 /label=BBa_K654060 promoter 3074..3108 /label=BBa_J23100 RBS 3117..3130 /label=BBa_B0035 misc_feature 3074..3130 /label=BBa_M1005 misc_feature 3137..3139 /label=Start Codon misc_feature 5258..5260 /label=Stop Codon CDS 3137..5257 /label=HlyB CDS 3137..5260 /label=BBa_M1003 RBS 5269..5282 /label=BBa_B0035 misc_feature 5289..5291 /label=Start Codon CDS 5289..6722 /label=HlyD misc_feature 6723..6725 /label=Stop Codon CDS 5289..6725 /label=BBa_M1004 misc_feature 6734..6772 /label=B1006 stem_loop 6743..6764 /label=modified thr terminator polyA_site 6765..6772 /label=PolyA polyA_site 6734..6742 /label=PolyA terminator 6734..6772 /label=BBa_B1006 misc_feature 3074..6772 /label=BBa_M1006 misc_feature 6785..6788 /label=conserved RBS 6781..6792 /label=BBa_B0034 ORIGIN 1 atgtttggtc ttattggtca tctcacgagt ttagaacacg cccaagcggt tgctgaagat 61 ttaggctatc ctgagtacgc caaccaaggc ctggattttt ggtgttcggc tcctccccaa 121 gtggttgata attttcaggt gaaaagtgtg acggggcagg tgattgaagg caaatatgtg 181 gagtcttgct ttttgccgga aatgttaacc caacggcgga tcaaagcggc cattcgtaaa 241 atcctcaatg ctatggccct ggcccaaaag gtgggcttgg atattacggc cctgggaggc 301 ttttcttcaa tcgtatttga agaatttaac ctcaagcaaa ataatcaagt ccgcaatgtg 361 gaactagatt ttcagcggtt caccactggt aatacccaca ccgcttatgt gatctgccgt 421 caggtcgagt ctggagctaa acagttgggt attgatctaa gtcaggcaac ggtagcggtt 481 tgtggcgcca cgggagatat tggtagcgcc gtatgtcgtt ggttagatag caaacatcaa 541 gttaaggaat tattgctaat tgcccgtaac cgccaaagat tggaaaatct ccaagaggaa 601 ttgggtcggg gcaaaattat ggatttggaa acagccctgc cccaggcaga tattattgtt 661 tgggtggcta gtatgcccaa gggggtagaa attgcggggg aaatgctgaa aaagccctgt 721 ttgattgtgg atgggggcta tcccaagaat ttagacacca gggtgaaagc ggatggggtg 781 catattctca agggggggat tgtagaacat tcccttgata ttacctggga aattatgaag 841 attgtggaga tggatattcc ctcccggcaa atgttcgcct gttttgcgga ggccattttg 901 ctagagtttg agggctggcg cactaatttt tcctggggcc gcaaccaaat ttccgttaat 961 aaaatggagg cgattggtga agcttctgtc aagcatggct tttgcccttt agtagctctt 1021 catcatcacc atcatcacca tcactagtac tagatgaatt atttcctgac aggcggcacc 1081 ggttttatcg gtcgttttct ggttgagaaa ctcttggcgc gcggcggcac cgtgtatgtt 1141 ctggttcgcg agcagtccca ggacaagctg gagcggctcc gggagcgctg gggtgcagac 1201 gacaagcaag tgaaggctgt gatcggcgac ctcaccagca aaaaccttgg tattgacgcg 1261 aaaacgctga aatcactgaa aggaaatatc gaccacgtat tccatcttgc cgcggtctac 1321 gacatgggcg cagacgaaga agcccaggcc gccaccaata tcgaaggcac cagggcggct 1381 gttcaggccg ccgaagccat gggcgccaag catttccatc atgtgtcatc catcgcggca 1441 gcgggtctgt tcaagggtat cttccgggag gatatgttcg aagaagccga gaagcttgat 1501 catccttacc tgcgcaccaa gcacgaatcc gaaaaagttg tgcgtgaaga atgcaaggtt 1561 ccgttccgca tctaccgccc tggtatggtc attggccatt cggaaaccgg cgaaatggac 1621 aaggttgacg ggccctatta cttcttcaag atgattcaga agatccgtca tgcgttgccc 1681 cagtgggtac ccaccatcgg tattgaaggt ggccggctga acattgtgcc ggtggatttc 1741 gtggtcgatg cactggatca cattgcccat ctggaaggcg aagatggcaa ctgtttccat 1801 ctggtggact ccgatccgta taaggtgggt gagatcctca atattttctg cgaggccggc 1861 catgcccccc gcatgggtat gcgcatcgat tcccggatgt tcggttttat tccgccgttt 1921 attcgccaga gcatcaagaa tctgcctccg gtcaagcgca ttactggtgc gcttctggat 1981 gacatgggca ttccgccctc ggtgatgtcc ttcattaatt acccgacccg ttttgatacc 2041 cgggagctgg agcgggttct gaagggcaca gacattgagg tgccgcgtct gccgtcctat 2101 gccccggtta tctgggacta ctgggagcgc aatctggacc cggacctgtt caaggaccgc 2161 accctcaagg gcacggttga aggtaaggtt tgcgtggtca ccggcgcgac ctcgggtatt 2221 ggcctggcaa cggcagagaa gctggcagag gccggtgcca ttctggtcat tggtgcgcgc 2281 accaaggaaa ctctggatga agtggcggcc agtctggagg ccaagggtgg caacgtgcat 2341 gcgtaccagt gcgacttttc ggacatggac gactgcgacc gctttgtgaa gacggtgctg 2401 gataatcacg gccacgtgga tgtactggtg aataacgcgg gtcgctccat ccgccgctcg 2461 ctggcgttgt cttttgaccg gttccacgat tttgagcgga ccatgcagct gaactacttt 2521 ggctccgttc ggctgatcat gggctttgcg ccagccatgc tggagcgtcg ccgcgggcac 2581 gtggtgaata tttcttccat cggggtactt accaacgctc cgcgtttctc ggcctatgtc 2641 tcctcgaaat ccgcactgga cgcgttcagc cgctgtgccg ctgccgaatg gtcggatcgc 2701 aacgtgacct tcaccaccat caacatgccg ttggtgaaaa cgccgatgat cgcgcccacc 2761 aagatctacg attccgtgcc gacgctgacg ccggatgaag ccgcccagat ggtggcggat 2821 gcgattgtgt accggcccaa gcgcattgcc acccgtcttg gcgtgttcgc gcaggttctg 2881 catgcgctgg caccgaagat gggtgagatc attatgaaca ctggctaccg gatgttcccg 2941 gattctccag cagccgctgg cagcaagtcc ggcgaaaagc cgaaagtctc taccgagcag 3001 gtggcctttg cggcgattat gcgggggata tactggagca tcatcaccat catcaccatc 3061 actaatacta gagttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta 3121 aagaggagaa tactagatgg attcctgtca caagattgat tacggcctgt atgcactgga 3181 aatcctggca caatatcata acgtctccgt caacccggag gaaattaaac atcgcttcga 3241 tactgacggc actggcctgg gtctgaccag ctggctgctg gcagcgaagt ccctggagct 3301 gaaggtaaag caagtgaaaa agacgattga tcgtctgaac ttcatcagcc tgccggcact 3361 ggtttggcgt gaagatggcc gtcacttcat cctgacgaaa gtgtccaaag aagcgaaccg 3421 ctacctgatc tttgatctgg agcagcgcaa cccgcgcgta ctggaacaga gcgagttcga 3481 ggctctgtat cagggccaca tcattctgat tgcatctcgt tcttctgttg caggtaaact 3541 ggcgaaattc gacttcacct ggttcattcc ggctattatc aagtaccgtc gtattttcat 3601 cgaaaccctg gttgtatctg ttttcctgca actgttcgct ctgattactc cgctgttctt 3661 tcaggttgtc atggacaaag ttctggttca ccgtggcttt tccaccctga acgtcattac 3721 tgttgcgctg agcgtggttg ttgtgttcga aatcattctg tccggtctgc gtacctacat 3781 cttcgcgcac tctacctccc gcatcgacgt tgaactgggt gcgaagctgt tccgtcacct 3841 gctggcactg cctatcagct acttcgaatc tcgtcgtgtg ggcgatactg tggcccgtgt 3901 tcgtgaactg gatcagatcc gcaactttct gaccggccag gccctgacca gcgttctgga 3961 tctgctgttc agctttatct tcttcgcagt aatgtggtac tacagcccga agctgactct 4021 ggtgattctg ttctctctgc cgtgttacgc agcttggtcc gtcttcatct ctccaatcct 4081 gcgtcgccgt ctggacgaca aatttagccg taatgcggat aatcagtcct tcctggtcga 4141 atctgtgact gctatcaaca ctatcaaagc catggcggtt tctccacaga tgactaacat 4201 ctgggataag cagctggcag gttacgttgc tgcgggcttc aaagtgaccg ttctggcaac 4261 cattggtcaa cagggcattc agctgattca gaaaaccgtg atgatcatta acctgtggct 4321 gggtgcgcac ctggtcatca gcggtgacct gtccattggc cagctgatcg ctttcaacat 4381 gctggcgggt caaattgttg ccccagtaat ccgtctggcc cagatctggc aggacttcca 4441 gcaggtcggt atttctgtga cccgcctggg tgacgtcctg aactctccga ccgaaagcta 4501 ccacggtaaa ctggctctgc cggagatcaa cggcgatatt actttccgta acatccgttt 4561 tcgttacaaa ccggattccc cggtaatcct ggataacatc aatctgtcta tcaaacaggg 4621 cgaagtcatc ggcatcgtgg gccgtagcgg cagcggcaag tccactctga ctaaactgat 4681 tcagcgcttt tacattccag aaaacggtca ggttctgatt gacggtcacg acctggctct 4741 ggcggacccg aactggctgc gtcgtcaggt gggcgtagtg ctgcaagata acgtgctgct 4801 gaaccgtagc atcatcgata acatcagcct ggctaacccg ggcatgtccg tggaaaaagt 4861 tatctatgct gcgaaactgg caggcgcgca cgatttcatc tctgaactgc gtgaaggcta 4921 caacaccatc gtaggcgaac agggcgctgg cctgtccggc ggtcaacgtc agcgtatcgc 4981 tattgcacgt gcactggtga acaacccgaa aatcctgatc ttcgacgagg ccacttctgc 5041 tctggattac gaatctgaac atattatcat gcgtaatatg cacaagatct gcaaaggtcg 5101 caccgtaatc atcatcgccc accgtctgag caccgtaaag aacgccgacc gcatcatcgt 5161 gatggaaaaa ggtaagatcg ttgaacaggg taaacataaa gagctgctgt ccgaaccgga 5221 atctctgtat tcttatctgt accagctgca atctgattaa tactagagat taaagaggag 5281 aatactagat gaaaacttgg ctgatgggct tctctgagtt cctgctgcgt tataagctgg 5341 tatggagcga aacttggaaa atccgtaagc aactggatac cccggtacgt gaaaaagacg 5401 aaaacgaatt tctgccagca catctggaac tgattgagac gccggtttct cgccgtccac 5461 gtctggttgc ttacttcatt atgggcttcc tggttattgc tgtaatcctg tctgttctgg 5521 gtcaggtgga aatcgttgcg accgcgaatg gtaaactgac cctgtccggt cgttctaaag 5581 aaatcaaacc gatcgaaaac tccatcgtta aagagattat cgttaaagaa ggtgaatccg 5641 tacgcaaagg cgatgttctg ctgaaactga ccgcactggg cgcagaagcg gacactctga 5701 aaactcagtc ctctctgctg caaacccgtc tggaacagac ccgttaccaa atcctgagcc 5761 gttctattga actgaacaaa ctgccggaac tgaaactgcc ggatgaacca tacttccaga 5821 acgtaagcga ggaggaagtt ctgcgcctga cttccctgat caaagagcag ttctctactt 5881 ggcagaacca gaaataccaa aaggaactga acctggacaa aaagcgtgcg gagcgcctga 5941 ctatcctggc ccgcatcaac cgttacgaga acctgtctcg tgttgaaaaa tctcgcctgg 6001 acgacttccg tagcctgctg cacaaacagg ccatcgcaaa acacgctgtg ctggaacagg 6061 aaaataaata cgtggaagct gccaacgaac tgcgtgtata taaaagccag ctggagcaga 6121 tcgagagcga aatcctgtct gcgaaggagg agtaccagct ggtgacgcag ctgttcaaaa 6181 atgaaatcct ggacaaactg cgtcaaacca cggactctat cgaactgctg accctggaac 6241 tggagaaaaa cgaggagcgc cagcaggcca gcgtcatccg cgctccggta tctggtaaag 6301 ttcagcaact gaaagttcac accgaaggtg gcgttgttac caccgcagaa accctgatgg 6361 ttattgtgcc ggaagatgac accctggaag ttaccgctct ggtgcagaac aaagatatcg 6421 gtttcatcaa cgtcggtcag aacgcgatca tcaaagtaga ggctttcccg tacactcgtt 6481 acggctacct ggtgggcaag gtcaaaaaca tcaacctgga cgctatcgaa gatcagaaac 6541 tgggcctggt gttcaacgtg atcgttagcg tagaagagaa cgatctgtct accggtaaca 6601 aacacattcc actgtcctcc ggcatggcgg ttactgctga aatcaaaacc ggtatgcgta 6661 gcgtgatctc ctatctgctg tctccgctgg aagaaagcgt caccgaatct ctgcacgaac 6721 gctaatacta gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tttactagag 6781 aaagaggaga aa //