LOCUS BBa_M1519 7214 bp DNA linear 29-Nov-2024 DEFINITION Antifreeze Protein (Variant YL2) ACCESSION BBa_M1519 VERSION BBa_M1519.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=Start misc_feature 1045..1047 /label=Stop CDS 1..1020 /label=Fatty Acyl ACP Reductase misc_feature 1021..1044 /label=8-His Tag CDS 1..1047 /label=BBa_K654062 misc_feature 1054..1056 /label=Start misc_feature 3063..3065 /label=Stop misc_feature 3039..3062 /label=8-His Tag misc_feature 2685..2685 /label=A>C CDS 1054..3038 /label=Fatty Acyl-CoA Reductase CDS 1054..3065 /label=BBa_K654060 RBS 3282..3293 /label=BBa_B0034 promoter 3074..3273 /label=BBa_R0010 promoter 3074..3293 /label=BBa_K208010 misc_feature 3302..3487 /label=HlyA misc_feature 3302..3304 /label=Start misc_feature 3302..3487 /label=BBa_K208006 promoter 3496..3530 /label=BBa_J23100 RBS 3539..3552 /label=BBa_B0035 misc_feature 3496..3552 /label=BBa_M1005 misc_feature 3559..3561 /label=Start Codon misc_feature 5680..5682 /label=Stop Codon CDS 3559..5679 /label=HlyB CDS 3559..5682 /label=BBa_M1003 RBS 5691..5704 /label=BBa_B0035 misc_feature 5711..5713 /label=Start Codon CDS 5711..7144 /label=HlyD misc_feature 7145..7147 /label=Stop Codon CDS 5711..7147 /label=BBa_M1004 misc_feature 7156..7194 /label=B1006 stem_loop 7165..7186 /label=modified thr terminator polyA_site 7187..7194 /label=PolyA polyA_site 7156..7164 /label=PolyA terminator 7156..7194 /label=BBa_B1006 misc_feature 3496..7194 /label=BBa_M1006 misc_feature 7207..7210 /label=conserved RBS 7203..7214 /label=BBa_B0034 ORIGIN 1 atgtttggtc ttattggtca tctcacgagt ttagaacacg cccaagcggt tgctgaagat 61 ttaggctatc ctgagtacgc caaccaaggc ctggattttt ggtgttcggc tcctccccaa 121 gtggttgata attttcaggt gaaaagtgtg acggggcagg tgattgaagg caaatatgtg 181 gagtcttgct ttttgccgga aatgttaacc caacggcgga tcaaagcggc cattcgtaaa 241 atcctcaatg ctatggccct ggcccaaaag gtgggcttgg atattacggc cctgggaggc 301 ttttcttcaa tcgtatttga agaatttaac ctcaagcaaa ataatcaagt ccgcaatgtg 361 gaactagatt ttcagcggtt caccactggt aatacccaca ccgcttatgt gatctgccgt 421 caggtcgagt ctggagctaa acagttgggt attgatctaa gtcaggcaac ggtagcggtt 481 tgtggcgcca cgggagatat tggtagcgcc gtatgtcgtt ggttagatag caaacatcaa 541 gttaaggaat tattgctaat tgcccgtaac cgccaaagat tggaaaatct ccaagaggaa 601 ttgggtcggg gcaaaattat ggatttggaa acagccctgc cccaggcaga tattattgtt 661 tgggtggcta gtatgcccaa gggggtagaa attgcggggg aaatgctgaa aaagccctgt 721 ttgattgtgg atgggggcta tcccaagaat ttagacacca gggtgaaagc ggatggggtg 781 catattctca agggggggat tgtagaacat tcccttgata ttacctggga aattatgaag 841 attgtggaga tggatattcc ctcccggcaa atgttcgcct gttttgcgga ggccattttg 901 ctagagtttg agggctggcg cactaatttt tcctggggcc gcaaccaaat ttccgttaat 961 aaaatggagg cgattggtga agcttctgtc aagcatggct tttgcccttt agtagctctt 1021 catcatcacc atcatcacca tcactagtac tagatgaatt atttcctgac aggcggcacc 1081 ggttttatcg gtcgttttct ggttgagaaa ctcttggcgc gcggcggcac cgtgtatgtt 1141 ctggttcgcg agcagtccca ggacaagctg gagcggctcc gggagcgctg gggtgcagac 1201 gacaagcaag tgaaggctgt gatcggcgac ctcaccagca aaaaccttgg tattgacgcg 1261 aaaacgctga aatcactgaa aggaaatatc gaccacgtat tccatcttgc cgcggtctac 1321 gacatgggcg cagacgaaga agcccaggcc gccaccaata tcgaaggcac cagggcggct 1381 gttcaggccg ccgaagccat gggcgccaag catttccatc atgtgtcatc catcgcggca 1441 gcgggtctgt tcaagggtat cttccgggag gatatgttcg aagaagccga gaagcttgat 1501 catccttacc tgcgcaccaa gcacgaatcc gaaaaagttg tgcgtgaaga atgcaaggtt 1561 ccgttccgca tctaccgccc tggtatggtc attggccatt cggaaaccgg cgaaatggac 1621 aaggttgacg ggccctatta cttcttcaag atgattcaga agatccgtca tgcgttgccc 1681 cagtgggtac ccaccatcgg tattgaaggt ggccggctga acattgtgcc ggtggatttc 1741 gtggtcgatg cactggatca cattgcccat ctggaaggcg aagatggcaa ctgtttccat 1801 ctggtggact ccgatccgta taaggtgggt gagatcctca atattttctg cgaggccggc 1861 catgcccccc gcatgggtat gcgcatcgat tcccggatgt tcggttttat tccgccgttt 1921 attcgccaga gcatcaagaa tctgcctccg gtcaagcgca ttactggtgc gcttctggat 1981 gacatgggca ttccgccctc ggtgatgtcc ttcattaatt acccgacccg ttttgatacc 2041 cgggagctgg agcgggttct gaagggcaca gacattgagg tgccgcgtct gccgtcctat 2101 gccccggtta tctgggacta ctgggagcgc aatctggacc cggacctgtt caaggaccgc 2161 accctcaagg gcacggttga aggtaaggtt tgcgtggtca ccggcgcgac ctcgggtatt 2221 ggcctggcaa cggcagagaa gctggcagag gccggtgcca ttctggtcat tggtgcgcgc 2281 accaaggaaa ctctggatga agtggcggcc agtctggagg ccaagggtgg caacgtgcat 2341 gcgtaccagt gcgacttttc ggacatggac gactgcgacc gctttgtgaa gacggtgctg 2401 gataatcacg gccacgtgga tgtactggtg aataacgcgg gtcgctccat ccgccgctcg 2461 ctggcgttgt cttttgaccg gttccacgat tttgagcgga ccatgcagct gaactacttt 2521 ggctccgttc ggctgatcat gggctttgcg ccagccatgc tggagcgtcg ccgcgggcac 2581 gtggtgaata tttcttccat cggggtactt accaacgctc cgcgtttctc ggcctatgtc 2641 tcctcgaaat ccgcactgga cgcgttcagc cgctgtgccg ctgccgaatg gtcggatcgc 2701 aacgtgacct tcaccaccat caacatgccg ttggtgaaaa cgccgatgat cgcgcccacc 2761 aagatctacg attccgtgcc gacgctgacg ccggatgaag ccgcccagat ggtggcggat 2821 gcgattgtgt accggcccaa gcgcattgcc acccgtcttg gcgtgttcgc gcaggttctg 2881 catgcgctgg caccgaagat gggtgagatc attatgaaca ctggctaccg gatgttcccg 2941 gattctccag cagccgctgg cagcaagtcc ggcgaaaagc cgaaagtctc taccgagcag 3001 gtggcctttg cggcgattat gcgggggata tactggagca tcatcaccat catcaccatc 3061 actaatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 3121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 3181 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 3241 tgtggaattg tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga 3301 gttagcctat ggaagtcagg gtgatcttaa tccattaatt aatgaaatca gcaaaatcat 3361 ttcagcagca ggtagcttcg atgttaaaga ggaaagaacc gcagcttctt tattgcagtt 3421 gtccggtaat gccagtgatt tttcatatgg acggaactca ataaccctga ccacatcagc 3481 ataataatac tagagttgac ggctagctca gtcctaggta cagtgctagc tactagagat 3541 taaagaggag aatactagat ggattcctgt cacaagattg attacggcct gtatgcactg 3601 gaaatcctgg cacaatatca taacgtctcc gtcaacccgg aggaaattaa acatcgcttc 3661 gatactgacg gcactggcct gggtctgacc agctggctgc tggcagcgaa gtccctggag 3721 ctgaaggtaa agcaagtgaa aaagacgatt gatcgtctga acttcatcag cctgccggca 3781 ctggtttggc gtgaagatgg ccgtcacttc atcctgacga aagtgtccaa agaagcgaac 3841 cgctacctga tctttgatct ggagcagcgc aacccgcgcg tactggaaca gagcgagttc 3901 gaggctctgt atcagggcca catcattctg attgcatctc gttcttctgt tgcaggtaaa 3961 ctggcgaaat tcgacttcac ctggttcatt ccggctatta tcaagtaccg tcgtattttc 4021 atcgaaaccc tggttgtatc tgttttcctg caactgttcg ctctgattac tccgctgttc 4081 tttcaggttg tcatggacaa agttctggtt caccgtggct tttccaccct gaacgtcatt 4141 actgttgcgc tgagcgtggt tgttgtgttc gaaatcattc tgtccggtct gcgtacctac 4201 atcttcgcgc actctacctc ccgcatcgac gttgaactgg gtgcgaagct gttccgtcac 4261 ctgctggcac tgcctatcag ctacttcgaa tctcgtcgtg tgggcgatac tgtggcccgt 4321 gttcgtgaac tggatcagat ccgcaacttt ctgaccggcc aggccctgac cagcgttctg 4381 gatctgctgt tcagctttat cttcttcgca gtaatgtggt actacagccc gaagctgact 4441 ctggtgattc tgttctctct gccgtgttac gcagcttggt ccgtcttcat ctctccaatc 4501 ctgcgtcgcc gtctggacga caaatttagc cgtaatgcgg ataatcagtc cttcctggtc 4561 gaatctgtga ctgctatcaa cactatcaaa gccatggcgg tttctccaca gatgactaac 4621 atctgggata agcagctggc aggttacgtt gctgcgggct tcaaagtgac cgttctggca 4681 accattggtc aacagggcat tcagctgatt cagaaaaccg tgatgatcat taacctgtgg 4741 ctgggtgcgc acctggtcat cagcggtgac ctgtccattg gccagctgat cgctttcaac 4801 atgctggcgg gtcaaattgt tgccccagta atccgtctgg cccagatctg gcaggacttc 4861 cagcaggtcg gtatttctgt gacccgcctg ggtgacgtcc tgaactctcc gaccgaaagc 4921 taccacggta aactggctct gccggagatc aacggcgata ttactttccg taacatccgt 4981 tttcgttaca aaccggattc cccggtaatc ctggataaca tcaatctgtc tatcaaacag 5041 ggcgaagtca tcggcatcgt gggccgtagc ggcagcggca agtccactct gactaaactg 5101 attcagcgct tttacattcc agaaaacggt caggttctga ttgacggtca cgacctggct 5161 ctggcggacc cgaactggct gcgtcgtcag gtgggcgtag tgctgcaaga taacgtgctg 5221 ctgaaccgta gcatcatcga taacatcagc ctggctaacc cgggcatgtc cgtggaaaaa 5281 gttatctatg ctgcgaaact ggcaggcgcg cacgatttca tctctgaact gcgtgaaggc 5341 tacaacacca tcgtaggcga acagggcgct ggcctgtccg gcggtcaacg tcagcgtatc 5401 gctattgcac gtgcactggt gaacaacccg aaaatcctga tcttcgacga ggccacttct 5461 gctctggatt acgaatctga acatattatc atgcgtaata tgcacaagat ctgcaaaggt 5521 cgcaccgtaa tcatcatcgc ccaccgtctg agcaccgtaa agaacgccga ccgcatcatc 5581 gtgatggaaa aaggtaagat cgttgaacag ggtaaacata aagagctgct gtccgaaccg 5641 gaatctctgt attcttatct gtaccagctg caatctgatt aatactagag attaaagagg 5701 agaatactag atgaaaactt ggctgatggg cttctctgag ttcctgctgc gttataagct 5761 ggtatggagc gaaacttgga aaatccgtaa gcaactggat accccggtac gtgaaaaaga 5821 cgaaaacgaa tttctgccag cacatctgga actgattgag acgccggttt ctcgccgtcc 5881 acgtctggtt gcttacttca ttatgggctt cctggttatt gctgtaatcc tgtctgttct 5941 gggtcaggtg gaaatcgttg cgaccgcgaa tggtaaactg accctgtccg gtcgttctaa 6001 agaaatcaaa ccgatcgaaa actccatcgt taaagagatt atcgttaaag aaggtgaatc 6061 cgtacgcaaa ggcgatgttc tgctgaaact gaccgcactg ggcgcagaag cggacactct 6121 gaaaactcag tcctctctgc tgcaaacccg tctggaacag acccgttacc aaatcctgag 6181 ccgttctatt gaactgaaca aactgccgga actgaaactg ccggatgaac catacttcca 6241 gaacgtaagc gaggaggaag ttctgcgcct gacttccctg atcaaagagc agttctctac 6301 ttggcagaac cagaaatacc aaaaggaact gaacctggac aaaaagcgtg cggagcgcct 6361 gactatcctg gcccgcatca accgttacga gaacctgtct cgtgttgaaa aatctcgcct 6421 ggacgacttc cgtagcctgc tgcacaaaca ggccatcgca aaacacgctg tgctggaaca 6481 ggaaaataaa tacgtggaag ctgccaacga actgcgtgta tataaaagcc agctggagca 6541 gatcgagagc gaaatcctgt ctgcgaagga ggagtaccag ctggtgacgc agctgttcaa 6601 aaatgaaatc ctggacaaac tgcgtcaaac cacggactct atcgaactgc tgaccctgga 6661 actggagaaa aacgaggagc gccagcaggc cagcgtcatc cgcgctccgg tatctggtaa 6721 agttcagcaa ctgaaagttc acaccgaagg tggcgttgtt accaccgcag aaaccctgat 6781 ggttattgtg ccggaagatg acaccctgga agttaccgct ctggtgcaga acaaagatat 6841 cggtttcatc aacgtcggtc agaacgcgat catcaaagta gaggctttcc cgtacactcg 6901 ttacggctac ctggtgggca aggtcaaaaa catcaacctg gacgctatcg aagatcagaa 6961 actgggcctg gtgttcaacg tgatcgttag cgtagaagag aacgatctgt ctaccggtaa 7021 caaacacatt ccactgtcct ccggcatggc ggttactgct gaaatcaaaa ccggtatgcg 7081 tagcgtgatc tcctatctgc tgtctccgct ggaagaaagc gtcaccgaat ctctgcacga 7141 acgctaatac tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttttactag 7201 agaaagagga gaaa //