BBa_M1519
type: ComponentDefinition
persistent identity: BBa M1519
display id: BBa_M1519
version: 1
was generated by: iGEM to SBOL conversion
description: Antifreeze Protein (Variant YL2)
created: 2012-04-16T11:00:00Z
modified: 2015-05-08T01:13:56Z
mutable provenance: Antifreeze protein. There are four variantes. This is YL2
owned by: myers
owned by: james
mutable description: Antifreeze protein. There are four variantes. This is YL2
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Antifreeze protein. There are four variantes. This is YL2
star: false
creator: sarah guzman
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K208006
component: BBa_K208010
component: BBa_B0034
component: BBa_M1006
component: BBa_K654060
component: BBa_K654062
sequence annotation: BBa_K654062
sequence annotation: BBa_K654060
sequence annotation: BBa_K208010
sequence annotation: BBa_K208006
sequence annotation: BBa_M1006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_M1519_sequence/1

BBa_K208006
type: Component
persistent identity: component2172283
display id: component2172283
version: 1
top level: BBa_M1519
definition: BBa_K208006
access: public

BBa_K208010
type: Component
persistent identity: component2172280
display id: component2172280
version: 1
top level: BBa_M1519
definition: BBa_K208010
access: public

BBa_B0034
type: Component
persistent identity: component2172303
display id: component2172303
version: 1
top level: BBa_M1519
definition: BBa_B0034
access: public

BBa_M1006
type: Component
persistent identity: component2172301
display id: component2172301
version: 1
top level: BBa_M1519
definition: BBa_M1006
access: public

BBa_K654060
type: Component
persistent identity: component2172277
display id: component2172277
version: 1
top level: BBa_M1519
definition: BBa_K654060
access: public

BBa_K654062
type: Component
persistent identity: component2172271
display id: component2172271
version: 1
top level: BBa_M1519
definition: BBa_K654062
access: public

BBa_K654062
type: SequenceAnnotation
persistent identity: annotation2172271
display id: annotation2172271
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172271/range2172271/1
component: BBa_K654062

https://synbiohub.org/public/igem/BBa_M1519/annotation2172271/range2172271/1
type: Range
persistent identity: range2172271
display id: range2172271
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 1
end: 1047
orientation: inline

BBa_K654060
type: SequenceAnnotation
persistent identity: annotation2172277
display id: annotation2172277
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172277/range2172277/1
component: BBa_K654060

https://synbiohub.org/public/igem/BBa_M1519/annotation2172277/range2172277/1
type: Range
persistent identity: range2172277
display id: range2172277
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 1054
end: 3065
orientation: inline

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2172280
display id: annotation2172280
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172280/range2172280/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_M1519/annotation2172280/range2172280/1
type: Range
persistent identity: range2172280
display id: range2172280
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 3074
end: 3293
orientation: inline

BBa_K208006
type: SequenceAnnotation
persistent identity: annotation2172283
display id: annotation2172283
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172283/range2172283/1
component: BBa_K208006

https://synbiohub.org/public/igem/BBa_M1519/annotation2172283/range2172283/1
type: Range
persistent identity: range2172283
display id: range2172283
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 3302
end: 3487
orientation: inline

BBa_M1006
type: SequenceAnnotation
persistent identity: annotation2172301
display id: annotation2172301
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172301/range2172301/1
component: BBa_M1006

https://synbiohub.org/public/igem/BBa_M1519/annotation2172301/range2172301/1
type: Range
persistent identity: range2172301
display id: range2172301
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 3496
end: 7194
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2172303
display id: annotation2172303
version: 1
top level: BBa_M1519
location: https://synbiohub.org/public/igem/BBa_M1519/annotation2172303/range2172303/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M1519/annotation2172303/range2172303/1
type: Range
persistent identity: range2172303
display id: range2172303
version: 1
top level: BBa_M1519
direction: direction/reverse
start: 7203
end: 7214
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K208006
type: ComponentDefinition
persistent identity: BBa K208006
display id: BBa_K208006
version: 1
was generated by: iGEM to SBOL conversion
description: HlyA Signal Peptide - Silver Fusion Compatible
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: Synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: HlyA
discontinued: false
dominant: true
experience: experience/None
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Silver Fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HlyA
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K208006_sequence/1

HlyA
type: SequenceAnnotation
persistent identity: annotation2034624
display id: annotation2034624
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K208006/annotation2034624/range2034624/1
type: Range
persistent identity: range2034624
display id: range2034624
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 186
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2062223
display id: annotation2062223
version: 1
top level: BBa_K208006
location: https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K208006/annotation2062223/range2062223/1
type: Range
persistent identity: range2062223
display id: range2062223
version: 1
top level: BBa_K208006
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K208010
type: ComponentDefinition
persistent identity: BBa K208010
display id: BBa_K208010
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Promoter (BBa_R0010) and RBS (B0034)
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lac + rbs
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Elisabeth Linton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K208010_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2034626
display id: annotation2034626
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K208010/annotation2034626/range2034626/1
type: Range
persistent identity: range2034626
display id: range2034626
version: 1
top level: BBa_K208010
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2034625
display id: annotation2034625
version: 1
top level: BBa_K208010
location: https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K208010/annotation2034625/range2034625/1
type: Range
persistent identity: range2034625
display id: range2034625
version: 1
top level: BBa_K208010
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_M1006
type: ComponentDefinition
persistent identity: BBa M1006
display id: BBa_M1006
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, RBS, HlyB, RBS, HlyD, Term
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: See subparts
owned by: james
owned by: myers
mutable description: Composite part for high levels of production of the HlyB and HlyD membrane proteins
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0035
component: BBa_M1003
component: BBa_M1005
component: BBa_B1006
component: BBa_M1004
sequence annotation: BBa_M1005
sequence annotation: BBa_M1003
sequence annotation: BBa_B0035
sequence annotation: BBa_M1004
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_M1006_sequence/1

BBa_B0035
type: Component
persistent identity: component2116004
display id: component2116004
version: 1
top level: BBa_M1006
definition: BBa_B0035
access: public

BBa_M1003
type: Component
persistent identity: component2116003
display id: component2116003
version: 1
top level: BBa_M1006
definition: BBa_M1003
access: public

BBa_M1005
type: Component
persistent identity: component2115999
display id: component2115999
version: 1
top level: BBa_M1006
definition: BBa_M1005
access: public

BBa_B1006
type: Component
persistent identity: component2116013
display id: component2116013
version: 1
top level: BBa_M1006
definition: BBa_B1006
access: public

BBa_M1004
type: Component
persistent identity: component2116008
display id: component2116008
version: 1
top level: BBa_M1006
definition: BBa_M1004
access: public

BBa_M1005
type: SequenceAnnotation
persistent identity: annotation2115999
display id: annotation2115999
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2115999/range2115999/1
component: BBa_M1005

https://synbiohub.org/public/igem/BBa_M1006/annotation2115999/range2115999/1
type: Range
persistent identity: range2115999
display id: range2115999
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2116003
display id: annotation2116003
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116003/range2116003/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1006/annotation2116003/range2116003/1
type: Range
persistent identity: range2116003
display id: range2116003
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 64
end: 2187
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2116004
display id: annotation2116004
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116004/range2116004/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1006/annotation2116004/range2116004/1
type: Range
persistent identity: range2116004
display id: range2116004
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 2196
end: 2209
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2116008
display id: annotation2116008
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116008/range2116008/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1006/annotation2116008/range2116008/1
type: Range
persistent identity: range2116008
display id: range2116008
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 2216
end: 3652
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2116013
display id: annotation2116013
version: 1
top level: BBa_M1006
location: https://synbiohub.org/public/igem/BBa_M1006/annotation2116013/range2116013/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_M1006/annotation2116013/range2116013/1
type: Range
persistent identity: range2116013
display id: range2116013
version: 1
top level: BBa_M1006
direction: direction/reverse
start: 3661
end: 3699
orientation: inline

BBa_K654060
type: ComponentDefinition
persistent identity: BBa K654060
display id: BBa_K654060
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl-CoA Reductase with 8-His Tag
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: Marinobacter aquaeolei genomic DNA
owned by: james
owned by: myers
mutable description: Maqu_2507 Fatty Acyl CoA Reductase (From Marinobacter aquaeolei) EC 1.2.1.50
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: mutagenesis removal of PstI site, without altering amino acid sequence
star: false
creator: Cody Tramp
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Fatty Acyl-CoA Reductase
sequence annotation: Start
sequence annotation: A>C
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654060_sequence/1

Fatty Acyl-CoA Reductase
type: SequenceAnnotation
persistent identity: annotation2141924
display id: annotation2141924
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141924/range2141924/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K654060/annotation2141924/range2141924/1
type: Range
persistent identity: range2141924
display id: range2141924
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1
end: 1985
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2141920
display id: annotation2141920
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141920/range2141920/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K654060/annotation2141920/range2141920/1
type: Range
persistent identity: range2141920
display id: range2141920
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1
end: 3
orientation: inline

A>C
type: SequenceAnnotation
persistent identity: annotation2141923
display id: annotation2141923
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141923/range2141923/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K654060/annotation2141923/range2141923/1
type: Range
persistent identity: range2141923
display id: range2141923
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1632
end: 1632
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2141922
display id: annotation2141922
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141922/range2141922/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K654060/annotation2141922/range2141922/1
type: Range
persistent identity: range2141922
display id: range2141922
version: 1
top level: BBa_K654060
direction: direction/forward
start: 1986
end: 2009
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2141921
display id: annotation2141921
version: 1
top level: BBa_K654060
location: https://synbiohub.org/public/igem/BBa_K654060/annotation2141921/range2141921/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K654060/annotation2141921/range2141921/1
type: Range
persistent identity: range2141921
display id: range2141921
version: 1
top level: BBa_K654060
direction: direction/forward
start: 2010
end: 2012
orientation: inline

BBa_K654062
type: ComponentDefinition
persistent identity: BBa K654062
display id: BBa_K654062
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl ACP Reductase with 8-His Tag
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: Synechocystis sp. PCC 6803 genomic DNA
owned by: james
owned by: myers
mutable description: sll0209 Fatty Acyl ACP Reductase (from Synechocystis sp. PCC 6803) EC 1.3.1.10
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Fatty Acyl ACP Reductase
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654062_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2141929
display id: annotation2141929
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141929/range2141929/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K654062/annotation2141929/range2141929/1
type: Range
persistent identity: range2141929
display id: range2141929
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1
end: 3
orientation: inline

Fatty Acyl ACP Reductase
type: SequenceAnnotation
persistent identity: annotation2141940
display id: annotation2141940
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141940/range2141940/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K654062/annotation2141940/range2141940/1
type: Range
persistent identity: range2141940
display id: range2141940
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1
end: 1020
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2141941
display id: annotation2141941
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141941/range2141941/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K654062/annotation2141941/range2141941/1
type: Range
persistent identity: range2141941
display id: range2141941
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1021
end: 1044
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2141930
display id: annotation2141930
version: 1
top level: BBa_K654062
location: https://synbiohub.org/public/igem/BBa_K654062/annotation2141930/range2141930/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K654062/annotation2141930/range2141930/1
type: Range
persistent identity: range2141930
display id: range2141930
version: 1
top level: BBa_K654062
direction: direction/forward
start: 1045
end: 1047
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

BBa_M1005
type: ComponentDefinition
persistent identity: BBa M1005
display id: BBa_M1005
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter+RBS
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Combination of registry parts.
owned by: james
owned by: myers
mutable description: Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23100
component: BBa_B0035
sequence annotation: BBa_J23100
sequence annotation: BBa_B0035
sequence: https://synbiohub.org/public/igem/BBa_M1005_sequence/1

BBa_J23100
type: Component
persistent identity: component2115955
display id: component2115955
version: 1
top level: BBa_M1005
definition: BBa_J23100
access: public

BBa_B0035
type: Component
persistent identity: component2115956
display id: component2115956
version: 1
top level: BBa_M1005
definition: BBa_B0035
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2115955
display id: annotation2115955
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_M1005/annotation2115955/range2115955/1
type: Range
persistent identity: range2115955
display id: range2115955
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2115956
display id: annotation2115956
version: 1
top level: BBa_M1005
location: https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1005/annotation2115956/range2115956/1
type: Range
persistent identity: range2115956
display id: range2115956
version: 1
top level: BBa_M1005
direction: direction/reverse
start: 44
end: 57
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_M1519_sequence/1
type: Sequence
persistent identity: BBa M1519 sequence
display id: BBa_M1519_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagatttaggctatcctgagtacgccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtgaaaagtgtgacggggcaggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggatcaaagcggccattcgtaaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggcttttcttcaatcgtatttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggttcaccactggtaatacccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatctaagtcaggcaacggtagcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacatcaagttaaggaattattgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaattatggatttggaaacagccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgcgggggaaatgctgaaaaagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggatggggtgcatattctcaagggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatggatattccctcccggcaaatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttcctggggccgcaaccaaatttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgccctttagtagctcttcatcatcaccatcatcaccatcactagtactagatgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttctggatgacatgggcattccgccctc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208006_sequence/1
type: Sequence
persistent identity: BBa K208006 sequence
display id: BBa_K208006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagcctatggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaagaggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctgaccacatcagcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208010_sequence/1
type: Sequence
persistent identity: BBa K208010 sequence
display id: BBa_K208010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1006_sequence/1
type: Sequence
persistent identity: BBa M1006 sequence
display id: BBa_M1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgtggttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654060_sequence/1
type: Sequence
persistent identity: BBa K654060 sequence
display id: BBa_K654060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttctggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgatacccgggagctggagcgggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctgggactactgggagcgcaatctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtggtcaccggcgcgacctcgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtgcgcgcaccaaggaaactctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcgacttttcggacatggacgactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaataacgcgggtcgctccatccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctgaactactttggctccgttcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatatttcttccatcggggtacttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagccgctgtgccgctgccgaatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatgatcgcgcccaccaagatctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgtgtaccggcccaagcgcattgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgagatcattatgaacactggctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaagtctctaccgagcaggtggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654062_sequence/1
type: Sequence
persistent identity: BBa K654062 sequence
display id: BBa_K654062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttggtcttattggtcatctcacgagtttagaacacgcccaagcggttgctgaagatttaggctatcctgagtacgccaaccaaggcctggatttttggtgttcggctcctccccaagtggttgataattttcaggtgaaaagtgtgacggggcaggtgattgaaggcaaatatgtggagtcttgctttttgccggaaatgttaacccaacggcggatcaaagcggccattcgtaaaatcctcaatgctatggccctggcccaaaaggtgggcttggatattacggccctgggaggcttttcttcaatcgtatttgaagaatttaacctcaagcaaaataatcaagtccgcaatgtggaactagattttcagcggttcaccactggtaatacccacaccgcttatgtgatctgccgtcaggtcgagtctggagctaaacagttgggtattgatctaagtcaggcaacggtagcggtttgtggcgccacgggagatattggtagcgccgtatgtcgttggttagatagcaaacatcaagttaaggaattattgctaattgcccgtaaccgccaaagattggaaaatctccaagaggaattgggtcggggcaaaattatggatttggaaacagccctgccccaggcagatattattgtttgggtggctagtatgcccaagggggtagaaattgcgggggaaatgctgaaaaagccctgtttgattgtggatgggggctatcccaagaatttagacaccagggtgaaagcggatggggtgcatattctcaagggggggattgtagaacattcccttgatattacctgggaaattatgaagattgtggagatggatattccctcccggcaaatgttcgcctgttttgcggaggccattttgctagagtttgagggctggcgcactaatttttcctggggccgcaaccaaatttccgttaataaaatggaggcgattggtgaagcttctgtcaagcatggcttttgccctttagtagctcttcatcatcaccatcatcaccatcactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1005_sequence/1
type: Sequence
persistent identity: BBa M1005 sequence
display id: BBa_M1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z