Antifreeze protein. There are four variantes. This is YL2
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
HlyA Signal Peptide - Silver Fusion Compatible
Lac Promoter (BBa_R0010) and RBS (B0034)
Composite part for high levels of production of the HlyB and HlyD membrane proteins
Maqu_2507 Fatty Acyl CoA Reductase (From Marinobacter aquaeolei) EC 1.2.1.50
mutagenesis removal of PstI site, without altering amino acid sequence
sll0209 Fatty Acyl ACP Reductase (from Synechocystis sp. PCC 6803) EC 1.3.1.10
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Membrane protein involved in type I secretion.
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
Constitutive promoter with high transcriptional levels reported. RBS has high level of translation.
Conversion of the iGEM parts registry to SBOL2.1