LOCUS       BBa_M1523               2013 bp    DNA     linear       22-Dec-2024
DEFINITION  Protease with promoter, RBS, and two transcription stops
ACCESSION   BBa_M1523
VERSION     BBa_M1523.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1874..1876
                     /label=stop
     CDS             227..1876
                     /label=cds
     CDS             227..1876
                     /label=BBa_M1522
     stem_loop       1896..1939
                     /label=stem_loop
     misc_feature    1885..1964
                     /label=BBa_B0010
     terminator      1885..1964
                     /label=BBa_B0010
     stem_loop       1980..1999
                     /label=T7 TE
     misc_feature    2006..2006
                     /label=stop
     polyA_site      2000..2013
                     /label=polya
     misc_feature    1973..2013
                     /label=BBa_B0012
     terminator      1973..2013
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tagagatacc
      241 gggctggata ccgttccagc ggctcgccgc gctcctcgcg cttctcgcgg ccgccgtcgc
      301 gctcgccgtc gtcgacagac cgagtcggct ctcggcggcg cttcggcggc gcttcctgtt
      361 cggtctcccg ctcggaacgc tcgccagcgc cggcggcgtc ctcgtcgtct atctcgtggt
      421 gcaagacggc tggtcgtcgt ggtaccggcc cgtcgtcatc ccgttccggg cgtggtcgta
      481 cttctacccg accgggatgc tgaccgccgc gttcgcccac tccagcgccg gccacctcgt
      541 cggcaacctc gtcggcacgc tcacgctcgc gccggtcgcg gagtacgcgt ggagccacta
      601 cccgacgcgc cgcgggtcca cctcgttcgg gtcggcccgc gagaacccct acgttcggtc
      661 gctcgtcgtc ttccccgcgg tcgtcttcgg cgtggggctc ctcaccgccg tgttcgcgct
      721 cggcccggtc gtcggcttct cgggcgtcgt gttcgcgttc gccggcttcg cgctcgtctt
      781 ccggccgctg gcgaccgtgc tggcgttcgt ctccgggcgc gtcgtcagcc tgttttacaa
      841 cgtgatgctc tcgcccgagg tcgtctcgtc ggcccgcccc gtcttctcga cgccgtggtg
      901 gtcccaaatc gccattcagg gccacgccat cggcttcctg ttcggcgtcc tcctcggtgc
      961 gtggctcagt caccgccgcg ggggttcgaa cccgcccgcg ctccgcagtt tcgccggcgt
     1021 cctcctgttt gccgtcagcg agtcgctgtg ggcggtgtac tggtaccgcg gcggcgagac
     1081 ctacgtgctg ttccgcgcgg tcgggttcgc gctcgtcgtc gcgctcgcga ccatcgtcgc
     1141 gctgacggtc gcggcgtccg acaagccgct tcgggcgtca cccgacaact cgctgttttc
     1201 ggcccgtcgc tggcaggccg gcctcgccgt gctcctcgtc gtcgtcgcgg cgctgtcggg
     1261 gccggcgatg ctgtacaaca cgttcaccgc gagcggcgac gacttgccgg gcgagagcgt
     1321 cacggtcagg gactacgagg tgacgtacgc cgaggacgtg ccgaacgggc tgaccgccgt
     1381 cttcgacgtc gaactgttcg gcgagtcgac gacgaccaac acctcgggag tcatcgtcaa
     1441 aagcgagcgg cgcggcatct ggacgacggc cgtctcgacg agtcggctgg cgttcgacgg
     1501 cgagtccgcg gtcagagtcg gcggtctcgg ctggcgcgac cgggtgacgg cggtccgcga
     1561 cggctacgtc gtcaccggcg cgggggtcgc gtaccgggtg ttcctcgtcg ccgacggcga
     1621 ggcgcggctg gcgtacgaga ccggcccggt ccgggccgga cccgtcgtcg cccggcggaa
     1681 cgtctcggtc gtgccgacgc cgacgggcta cgacgtgcag gtgtcgtccg acagcgggac
     1741 cgtcaggggg ccgatgccga ccgggaacac gacgacgacg ctcgacggga ttcggttcgt
     1801 ccgcgagaac tccctcgtct tcgccgaatc ccgggggacg aaagtccgca tcgcgcgaca
     1861 ggagacgtac aattagtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1921 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1981 gctcaccttc gggtgggcct ttctgcgttt ata
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