##gff-version 3
##sequence-region BBa_M1523 1 2013
BBa_M1523	.	terminator	1885	1964	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_M1523	.	engineered_region	1885	1964	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1523	.	stem_loop	1896	1939	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1523	.	CDS	227	1876	.	+	0	ID=BBa_M1522;Name=BBa_M1522
BBa_M1523	.	CDS	227	1876	.	+	0	ID=annotation2170956;Name=cds;Parent=BBa_M1522
BBa_M1523	.	start_codon	227	229	.	+	0	ID=annotation2170954;Name=start;Parent=BBa_M1522
BBa_M1523	.	stop_codon	1874	1876	.	+	0	ID=annotation2170955;Name=stop;Parent=BBa_M1522
BBa_M1523	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1523	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1523	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_M1523	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_M1523	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_M1523	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_M1523	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_M1523	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_M1523	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_M1523	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_M1523	.	terminator	1973	2013	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_M1523	.	stop_codon	2006	2006	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1523	.	polyA_site	2000	2013	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1523	.	stem_loop	1980	1999	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1523	.	engineered_region	1973	2013	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_M1523
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctagagat
accgggctggataccgttccagcggctcgccgcgctcctcgcgcttctcgcggccgccgtcgcgctcgccgtcgtcgac
agaccgagtcggctctcggcggcgcttcggcggcgcttcctgttcggtctcccgctcggaacgctcgccagcgccggcg
gcgtcctcgtcgtctatctcgtggtgcaagacggctggtcgtcgtggtaccggcccgtcgtcatcccgttccgggcgtg
gtcgtacttctacccgaccgggatgctgaccgccgcgttcgcccactccagcgccggccacctcgtcggcaacctcgtc
ggcacgctcacgctcgcgccggtcgcggagtacgcgtggagccactacccgacgcgccgcgggtccacctcgttcgggt
cggcccgcgagaacccctacgttcggtcgctcgtcgtcttccccgcggtcgtcttcggcgtggggctcctcaccgccgt
gttcgcgctcggcccggtcgtcggcttctcgggcgtcgtgttcgcgttcgccggcttcgcgctcgtcttccggccgctg
gcgaccgtgctggcgttcgtctccgggcgcgtcgtcagcctgttttacaacgtgatgctctcgcccgaggtcgtctcgt
cggcccgccccgtcttctcgacgccgtggtggtcccaaatcgccattcagggccacgccatcggcttcctgttcggcgt
cctcctcggtgcgtggctcagtcaccgccgcgggggttcgaacccgcccgcgctccgcagtttcgccggcgtcctcctg
tttgccgtcagcgagtcgctgtgggcggtgtactggtaccgcggcggcgagacctacgtgctgttccgcgcggtcgggt
tcgcgctcgtcgtcgcgctcgcgaccatcgtcgcgctgacggtcgcggcgtccgacaagccgcttcgggcgtcacccga
caactcgctgttttcggcccgtcgctggcaggccggcctcgccgtgctcctcgtcgtcgtcgcggcgctgtcggggccg
gcgatgctgtacaacacgttcaccgcgagcggcgacgacttgccgggcgagagcgtcacggtcagggactacgaggtga
cgtacgccgaggacgtgccgaacgggctgaccgccgtcttcgacgtcgaactgttcggcgagtcgacgacgaccaacac
ctcgggagtcatcgtcaaaagcgagcggcgcggcatctggacgacggccgtctcgacgagtcggctggcgttcgacggc
gagtccgcggtcagagtcggcggtctcggctggcgcgaccgggtgacggcggtccgcgacggctacgtcgtcaccggcg
cgggggtcgcgtaccgggtgttcctcgtcgccgacggcgaggcgcggctggcgtacgagaccggcccggtccgggccgg
acccgtcgtcgcccggcggaacgtctcggtcgtgccgacgccgacgggctacgacgtgcaggtgtcgtccgacagcggg
accgtcagggggccgatgccgaccgggaacacgacgacgacgctcgacgggattcggttcgtccgcgagaactccctcg
tcttcgccgaatcccgggggacgaaagtccgcatcgcgcgacaggagacgtacaattagtactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata