LOCUS       BBa_M1526                902 bp    DNA     linear       24-Nov-2024
DEFINITION  sgd1
ACCESSION   BBa_M1526
VERSION     BBa_M1526.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    203..425
                     /label=misc
     misc_feature    88..107
                     /label=misc
     misc_feature    81..765
                     /label=BBa_M1527
     stem_loop       785..828
                     /label=stem_loop
     misc_feature    774..853
                     /label=BBa_B0010
     terminator      774..853
                     /label=BBa_B0010
     stem_loop       869..888
                     /label=T7 TE
     misc_feature    895..895
                     /label=stop
     polyA_site      889..902
                     /label=polya
     misc_feature    862..902
                     /label=BBa_B0012
     terminator      862..902
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      121 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      181 tcgagctgaa cctgcaggtg tcaagacttc gttcgcagaa ggcgttgaca acgccaagaa
      241 ggcgctgtcg gccaaggacg cacaggaact gctggccatc caggccgcag ccgtgcagcc
      301 ggttgccgaa aagaccctgg cctacacccg ccacctgtat gaaatcgctt cggaaaccca
      361 gagcgagttc accaaggtag ccgaggctca actggccgaa ggctcgaaga acgtgcaagc
      421 gctggtcgag aacctcgcca agaacgcccc ggccggttcg gaatcgaccg tggccatcgt
      481 gaagtcggcg atctccgctg ccaacaacgc ctacgagtcg gtgcagaagg cgaccaagca
      541 agcggtcgaa atcgctgaaa ccaacttcca ggctgcggct acggctgcca ccaaggctgc
      601 ccagcaagcc agcgccacgg cccgtacggc cacggcaaag aagacgacgg ctgcctgata
      661 actgcctgcg ttgaagatgg accggctgcg gccggtccgt tggcaaagca tatcgacgcc
      721 tggcgtttgc ggtgtgtttt gccaacgatg aaggtagtgc cctgatacta gagccaggca
      781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      901 ta
//