LOCUS BBa_M1530 9238 bp DNA linear 22-Dec-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1530 VERSION BBa_M1530.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 229..414 /label=HlyA misc_feature 229..231 /label=Start misc_feature 229..414 /label=BBa_K208006 CDS 421..897 /label=cds CDS 421..927 /label=BBa_M1525 RBS 936..949 /label=BBa_B0035 misc_feature 956..958 /label=Start Codon misc_feature 3077..3079 /label=Stop Codon CDS 956..3076 /label=HlyB CDS 956..3079 /label=BBa_M1003 RBS 3088..3101 /label=BBa_B0035 misc_feature 3108..3110 /label=Start Codon CDS 3108..4541 /label=HlyD misc_feature 4542..4544 /label=Stop Codon CDS 3108..4544 /label=BBa_M1004 misc_feature 936..4544 /label=BBa_M1528 misc_feature 4557..4560 /label=conserved RBS 4553..4564 /label=BBa_B0034 misc_feature 4573..4758 /label=HlyA misc_feature 4573..4575 /label=Start misc_feature 4573..4758 /label=BBa_K208006 misc_feature 4765..4767 /label=Start CDS 4765..5484 /label=GFP Coding Region CDS 4765..5484 /label=BBa_K208000 RBS 5493..5506 /label=BBa_B0035 misc_feature 5513..5515 /label=Start Codon misc_feature 7634..7636 /label=Stop Codon CDS 5513..7633 /label=HlyB CDS 5513..7636 /label=BBa_M1003 RBS 7645..7658 /label=BBa_B0035 misc_feature 7665..7667 /label=Start Codon CDS 7665..9098 /label=HlyD misc_feature 9099..9101 /label=Stop Codon CDS 7665..9101 /label=BBa_M1004 misc_feature 5493..9101 /label=BBa_M1528 stem_loop 9121..9164 /label=stem_loop misc_feature 9110..9189 /label=BBa_B0010 terminator 9110..9189 /label=BBa_B0010 stem_loop 9205..9224 /label=T7 TE misc_feature 9231..9231 /label=stop polyA_site 9225..9238 /label=polya misc_feature 9198..9238 /label=BBa_B0012 terminator 9198..9238 /label=BBa_B0012 terminator 9110..9238 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt agcctatgga 241 agtcagggtg atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt 301 agcttcgatg ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc 361 agtgattttt catatggacg gaactcaata accctgacca catcagcata ataatactag 421 atgtctttta aaattagcac ctttaccaaa atctggctca ttatcgcggt catcgtcatg 481 tgtctgtgca atgaatacaa ctgtcagtgt acgggggccg cagattgcac atcgtgcacg 541 gcagcatgca ctggttgtgg caattgtccg aacgcgatta cctgtaccgg aagcaagaat 601 tgcgtgcgcg caacaacttg tactgatagc gaaaactgcg ttaaagcaca tacttgtaca 661 ggatcgacaa attgcaacag agcgaccacc tgtactaaca gcaaggggtg cttggaggcc 721 acgacgtgca ctggttctac tcattgtcat cgcgcaacta cgtgcacaaa tagcaaagat 781 tgttttgaag ctaccacatg tactgggtca tcaaactgct ataccgctac cacctgcact 841 aatagcacga actgctataa agctactgca tgtacaaata gtacggggtg cccagggcat 901 catcatcacc atcatcacca tcactagtac tagagattaa agaggagaat actagatgga 961 ttcctgtcac aagattgatt acggcctgta tgcactggaa atcctggcac aatatcataa 1021 cgtctccgtc aacccggagg aaattaaaca tcgcttcgat actgacggca ctggcctggg 1081 tctgaccagc tggctgctgg cagcgaagtc cctggagctg aaggtaaagc aagtgaaaaa 1141 gacgattgat cgtctgaact tcatcagcct gccggcactg gtttggcgtg aagatggccg 1201 tcacttcatc ctgacgaaag tgtccaaaga agcgaaccgc tacctgatct ttgatctgga 1261 gcagcgcaac ccgcgcgtac tggaacagag cgagttcgag gctctgtatc agggccacat 1321 cattctgatt gcatctcgtt cttctgttgc aggtaaactg gcgaaattcg acttcacctg 1381 gttcattccg gctattatca agtaccgtcg tattttcatc gaaaccctgg ttgtatctgt 1441 tttcctgcaa ctgttcgctc tgattactcc gctgttcttt caggttgtca tggacaaagt 1501 tctggttcac cgtggctttt ccaccctgaa cgtcattact gttgcgctga gcgttgttgt 1561 tgtgttcgaa atcattctgt ccggtctgcg tacctacatc ttcgcgcact ctacctcccg 1621 catcgacgtt gaactgggtg cgaagctgtt ccgtcacctg ctggcactgc ctatcagcta 1681 cttcgaatct cgtcgtgtgg gcgatactgt ggcccgtgtt cgtgaactgg atcagatccg 1741 caactttctg accggccagg ccctgaccag cgttctggat ctgctgttca gctttatctt 1801 cttcgcagta atgtggtact acagcccgaa gctgactctg gtgattctgt tctctctgcc 1861 gtgttacgca gcttggtccg tcttcatctc tccaatcctg cgtcgccgtc tggacgacaa 1921 atttagccgt aatgcggata atcagtcctt cctggtcgaa tctgtgactg ctatcaacac 1981 tatcaaagcc atggcggttt ctccacagat gactaacatc tgggataagc agctggcagg 2041 ttacgttgct gcgggcttca aagtgaccgt tctggcaacc attggtcaac agggcattca 2101 gctgattcag aaaaccgtga tgatcattaa cctgtggctg ggtgcgcacc tggtcatcag 2161 cggtgacctg tccattggcc agctgatcgc tttcaacatg ctggcgggtc aaattgttgc 2221 cccagtaatc cgtctggccc agatctggca ggacttccag caggtcggta tttctgtgac 2281 ccgcctgggt gacgtcctga actctccgac cgaaagctac cacggtaaac tggctctgcc 2341 ggagatcaac ggcgatatta ctttccgtaa catccgtttt cgttacaaac cggattcccc 2401 ggtaatcctg gataacatca atctgtctat caaacagggc gaagtcatcg gcatcgtggg 2461 ccgtagcggc agcggcaagt ccactctgac taaactgatt cagcgctttt acattccaga 2521 aaacggtcag gttctgattg acggtcacga cctggctctg gcggacccga actggctgcg 2581 tcgtcaggtg ggcgtagtgc tgcaagataa cgtgctgctg aaccgtagca tcatcgataa 2641 catcagcctg gctaacccgg gcatgtccgt ggaaaaagtt atctatgctg cgaaactggc 2701 aggcgcgcac gatttcatct ctgaactgcg tgaaggctac aacaccatcg taggcgaaca 2761 gggcgctggc ctgtccggcg gtcaacgtca gcgtatcgct attgcacgtg cactggtgaa 2821 caacccgaaa atcctgatct tcgacgaggc cacttctgct ctggattacg aatctgaaca 2881 tattatcatg cgtaatatgc acaagatctg caaaggtcgc accgtaatca tcatcgccca 2941 ccgtctgagc accgtaaaga acgccgaccg catcatcgtg atggaaaaag gtaagatcgt 3001 tgaacagggt aaacataaag agctgctgtc cgaaccggaa tctctgtatt cttatctgta 3061 ccagctgcaa tctgattaat actagagatt aaagaggaga atactagatg aaaacttggc 3121 tgatgggctt ctctgagttc ctgctgcgtt ataagctggt atggagcgaa acttggaaaa 3181 tccgtaagca actggatacc ccggtacgtg aaaaagacga aaacgaattt ctgccagcac 3241 atctggaact gattgagacg ccggtttctc gccgtccacg tctggttgct tacttcatta 3301 tgggcttcct ggttattgct gtaatcctgt ctgttctggg tcaggtggaa atcgttgcga 3361 ccgcgaatgg taaactgacc ctgtccggtc gttctaaaga aatcaaaccg atcgaaaact 3421 ccatcgttaa agagattatc gttaaagaag gtgaatccgt acgcaaaggc gatgttctgc 3481 tgaaactgac cgcactgggc gcagaagcgg acactctgaa aactcagtcc tctctgctgc 3541 aaacccgtct ggaacagacc cgttaccaaa tcctgagccg ttctattgaa ctgaacaaac 3601 tgccggaact gaaactgccg gatgaaccat acttccagaa cgtaagcgag gaggaagttc 3661 tgcgcctgac ttccctgatc aaagagcagt tctctacttg gcagaaccag aaataccaaa 3721 aggaactgaa cctggacaaa aagcgtgcgg agcgcctgac tatcctggcc cgcatcaacc 3781 gttacgagaa cctgtctcgt gttgaaaaat ctcgcctgga cgacttccgt agcctgctgc 3841 acaaacaggc catcgcaaaa cacgctgtgc tggaacagga aaataaatac gtggaagctg 3901 ccaacgaact gcgtgtatat aaaagccagc tggagcagat cgagagcgaa atcctgtctg 3961 cgaaggagga gtaccagctg gtgacgcagc tgttcaaaaa tgaaatcctg gacaaactgc 4021 gtcaaaccac ggactctatc gaactgctga ccctggaact ggagaaaaac gaggagcgcc 4081 agcaggccag cgtcatccgc gctccggtat ctggtaaagt tcagcaactg aaagttcaca 4141 ccgaaggtgg cgttgttacc accgcagaaa ccctgatggt tattgtgccg gaagatgaca 4201 ccctggaagt taccgctctg gtgcagaaca aagatatcgg tttcatcaac gtcggtcaga 4261 acgcgatcat caaagtagag gctttcccgt acactcgtta cggctacctg gtgggcaagg 4321 tcaaaaacat caacctggac gctatcgaag atcagaaact gggcctggtg ttcaacgtga 4381 tcgttagcgt agaagagaac gatctgtcta ccggtaacaa acacattcca ctgtcctccg 4441 gcatggcggt tactgctgaa atcaaaaccg gtatgcgtag cgtgatctcc tatctgctgt 4501 ctccgctgga agaaagcgtc accgaatctc tgcacgaacg ctaatactag agaaagagga 4561 gaaatactag agttagccta tggaagtcag ggtgatctta atccattaat taatgaaatc 4621 agcaaaatca tttcagcagc aggtagcttc gatgttaaag aggaaagaac cgcagcttct 4681 ttattgcagt tgtccggtaa tgccagtgat ttttcatatg gacggaactc aataaccctg 4741 accacatcag cataataata ctagatggct agcaaaggag aagaactttt cactggagtt 4801 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 4861 gagggtgaag gtgatgctac atacggaaag cttaccctta aatttatttg cactactgga 4921 aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcaatgcttt 4981 tcccgttatc cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt 5041 tatgtacagg aacgcactat atctttcaaa gatgacggga actacaagac gcgtgctgaa 5101 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaaaggtat tgattttaaa 5161 gaagatggaa acattctcgg acacaaactc gagtacaact ataactcaca caatgtatac 5221 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacatt 5281 gaagatggat ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 5341 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgccctttc gaaagatccc 5401 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat 5461 ggcatggatg agctctacaa ataatactag agattaaaga ggagaatact agatggattc 5521 ctgtcacaag attgattacg gcctgtatgc actggaaatc ctggcacaat atcataacgt 5581 ctccgtcaac ccggaggaaa ttaaacatcg cttcgatact gacggcactg gcctgggtct 5641 gaccagctgg ctgctggcag cgaagtccct ggagctgaag gtaaagcaag tgaaaaagac 5701 gattgatcgt ctgaacttca tcagcctgcc ggcactggtt tggcgtgaag atggccgtca 5761 cttcatcctg acgaaagtgt ccaaagaagc gaaccgctac ctgatctttg atctggagca 5821 gcgcaacccg cgcgtactgg aacagagcga gttcgaggct ctgtatcagg gccacatcat 5881 tctgattgca tctcgttctt ctgttgcagg taaactggcg aaattcgact tcacctggtt 5941 cattccggct attatcaagt accgtcgtat tttcatcgaa accctggttg tatctgtttt 6001 cctgcaactg ttcgctctga ttactccgct gttctttcag gttgtcatgg acaaagttct 6061 ggttcaccgt ggcttttcca ccctgaacgt cattactgtt gcgctgagcg ttgttgttgt 6121 gttcgaaatc attctgtccg gtctgcgtac ctacatcttc gcgcactcta cctcccgcat 6181 cgacgttgaa ctgggtgcga agctgttccg tcacctgctg gcactgccta tcagctactt 6241 cgaatctcgt cgtgtgggcg atactgtggc ccgtgttcgt gaactggatc agatccgcaa 6301 ctttctgacc ggccaggccc tgaccagcgt tctggatctg ctgttcagct ttatcttctt 6361 cgcagtaatg tggtactaca gcccgaagct gactctggtg attctgttct ctctgccgtg 6421 ttacgcagct tggtccgtct tcatctctcc aatcctgcgt cgccgtctgg acgacaaatt 6481 tagccgtaat gcggataatc agtccttcct ggtcgaatct gtgactgcta tcaacactat 6541 caaagccatg gcggtttctc cacagatgac taacatctgg gataagcagc tggcaggtta 6601 cgttgctgcg ggcttcaaag tgaccgttct ggcaaccatt ggtcaacagg gcattcagct 6661 gattcagaaa accgtgatga tcattaacct gtggctgggt gcgcacctgg tcatcagcgg 6721 tgacctgtcc attggccagc tgatcgcttt caacatgctg gcgggtcaaa ttgttgcccc 6781 agtaatccgt ctggcccaga tctggcagga cttccagcag gtcggtattt ctgtgacccg 6841 cctgggtgac gtcctgaact ctccgaccga aagctaccac ggtaaactgg ctctgccgga 6901 gatcaacggc gatattactt tccgtaacat ccgttttcgt tacaaaccgg attccccggt 6961 aatcctggat aacatcaatc tgtctatcaa acagggcgaa gtcatcggca tcgtgggccg 7021 tagcggcagc ggcaagtcca ctctgactaa actgattcag cgcttttaca ttccagaaaa 7081 cggtcaggtt ctgattgacg gtcacgacct ggctctggcg gacccgaact ggctgcgtcg 7141 tcaggtgggc gtagtgctgc aagataacgt gctgctgaac cgtagcatca tcgataacat 7201 cagcctggct aacccgggca tgtccgtgga aaaagttatc tatgctgcga aactggcagg 7261 cgcgcacgat ttcatctctg aactgcgtga aggctacaac accatcgtag gcgaacaggg 7321 cgctggcctg tccggcggtc aacgtcagcg tatcgctatt gcacgtgcac tggtgaacaa 7381 cccgaaaatc ctgatcttcg acgaggccac ttctgctctg gattacgaat ctgaacatat 7441 tatcatgcgt aatatgcaca agatctgcaa aggtcgcacc gtaatcatca tcgcccaccg 7501 tctgagcacc gtaaagaacg ccgaccgcat catcgtgatg gaaaaaggta agatcgttga 7561 acagggtaaa cataaagagc tgctgtccga accggaatct ctgtattctt atctgtacca 7621 gctgcaatct gattaatact agagattaaa gaggagaata ctagatgaaa acttggctga 7681 tgggcttctc tgagttcctg ctgcgttata agctggtatg gagcgaaact tggaaaatcc 7741 gtaagcaact ggataccccg gtacgtgaaa aagacgaaaa cgaatttctg ccagcacatc 7801 tggaactgat tgagacgccg gtttctcgcc gtccacgtct ggttgcttac ttcattatgg 7861 gcttcctggt tattgctgta atcctgtctg ttctgggtca ggtggaaatc gttgcgaccg 7921 cgaatggtaa actgaccctg tccggtcgtt ctaaagaaat caaaccgatc gaaaactcca 7981 tcgttaaaga gattatcgtt aaagaaggtg aatccgtacg caaaggcgat gttctgctga 8041 aactgaccgc actgggcgca gaagcggaca ctctgaaaac tcagtcctct ctgctgcaaa 8101 cccgtctgga acagacccgt taccaaatcc tgagccgttc tattgaactg aacaaactgc 8161 cggaactgaa actgccggat gaaccatact tccagaacgt aagcgaggag gaagttctgc 8221 gcctgacttc cctgatcaaa gagcagttct ctacttggca gaaccagaaa taccaaaagg 8281 aactgaacct ggacaaaaag cgtgcggagc gcctgactat cctggcccgc atcaaccgtt 8341 acgagaacct gtctcgtgtt gaaaaatctc gcctggacga cttccgtagc ctgctgcaca 8401 aacaggccat cgcaaaacac gctgtgctgg aacaggaaaa taaatacgtg gaagctgcca 8461 acgaactgcg tgtatataaa agccagctgg agcagatcga gagcgaaatc ctgtctgcga 8521 aggaggagta ccagctggtg acgcagctgt tcaaaaatga aatcctggac aaactgcgtc 8581 aaaccacgga ctctatcgaa ctgctgaccc tggaactgga gaaaaacgag gagcgccagc 8641 aggccagcgt catccgcgct ccggtatctg gtaaagttca gcaactgaaa gttcacaccg 8701 aaggtggcgt tgttaccacc gcagaaaccc tgatggttat tgtgccggaa gatgacaccc 8761 tggaagttac cgctctggtg cagaacaaag atatcggttt catcaacgtc ggtcagaacg 8821 cgatcatcaa agtagaggct ttcccgtaca ctcgttacgg ctacctggtg ggcaaggtca 8881 aaaacatcaa cctggacgct atcgaagatc agaaactggg cctggtgttc aacgtgatcg 8941 ttagcgtaga agagaacgat ctgtctaccg gtaacaaaca cattccactg tcctccggca 9001 tggcggttac tgctgaaatc aaaaccggta tgcgtagcgt gatctcctat ctgctgtctc 9061 cgctggaaga aagcgtcacc gaatctctgc acgaacgcta atactagagc caggcatcaa 9121 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 9181 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //