##gff-version 3
##sequence-region BBa_M1530 1 9238
BBa_M1530	.	tag	4573	4758	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1530	.	start_codon	4573	4575	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1530	.	tag	4573	4758	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1530	.	tag	229	414	.	+	0	ID=BBa_K208006;Name=BBa_K208006
BBa_M1530	.	start_codon	229	231	.	+	0	ID=annotation2062223;Name=Start;Parent=BBa_K208006
BBa_M1530	.	tag	229	414	.	+	0	ID=annotation2034624;Name=HlyA;Parent=BBa_K208006
BBa_M1530	.	engineered_region	936	4544	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1530	.	ribosome_entry_site	3088	3101	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1530	.	ribosome_entry_site	936	949	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1530	.	CDS	956	3079	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1530	.	CDS	956	3076	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1530	.	stop_codon	3077	3079	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1530	.	start_codon	956	958	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1530	.	CDS	3108	4544	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1530	.	CDS	3108	4541	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1530	.	start_codon	3108	3110	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1530	.	stop_codon	4542	4544	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_M1530	.	CDS	421	927	.	+	0	ID=BBa_M1525;Name=BBa_M1525
BBa_M1530	.	CDS	421	897	.	+	0	ID=annotation2172676;Name=cds;Parent=BBa_M1525
BBa_M1530	.	promoter	1	220	.	+	0	ID=BBa_K208010;Name=BBa_K208010
BBa_M1530	.	ribosome_entry_site	209	220	.	+	0	ID=annotation2034625;Name=BBa_B0034;Parent=BBa_K208010
BBa_M1530	.	promoter	1	200	.	+	0	ID=annotation2034626;Name=BBa_R0010;Parent=BBa_K208010
BBa_M1530	.	CDS	4765	5484	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_M1530	.	CDS	4765	5484	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_M1530	.	start_codon	4765	4767	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_M1530	.	ribosome_entry_site	4553	4564	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_M1530	.	conserved_region	4557	4560	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_M1530	.	terminator	9110	9238	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M1530	.	terminator	9198	9238	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M1530	.	polyA_site	9225	9238	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M1530	.	stem_loop	9205	9224	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M1530	.	stop_codon	9231	9231	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M1530	.	engineered_region	9198	9238	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M1530	.	terminator	9110	9189	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M1530	.	stem_loop	9121	9164	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M1530	.	engineered_region	9110	9189	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M1530	.	engineered_region	5493	9101	.	+	0	ID=BBa_M1528;Name=BBa_M1528
BBa_M1530	.	ribosome_entry_site	7645	7658	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1530	.	ribosome_entry_site	5493	5506	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1528
BBa_M1530	.	CDS	5513	7636	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1528
BBa_M1530	.	CDS	5513	7633	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_M1530	.	stop_codon	7634	7636	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_M1530	.	start_codon	5513	5515	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_M1530	.	CDS	7665	9101	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1528
BBa_M1530	.	CDS	7665	9098	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_M1530	.	start_codon	7665	7667	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_M1530	.	stop_codon	9099	9101	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
>BBa_M1530
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttagcctat
ggaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaag
aggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccct
gaccacatcagcataataatactagatgtcttttaaaattagcacctttaccaaaatctggctcattatcgcggtcatc
gtcatgtgtctgtgcaatgaatacaactgtcagtgtacgggggccgcagattgcacatcgtgcacggcagcatgcactg
gttgtggcaattgtccgaacgcgattacctgtaccggaagcaagaattgcgtgcgcgcaacaacttgtactgatagcga
aaactgcgttaaagcacatacttgtacaggatcgacaaattgcaacagagcgaccacctgtactaacagcaaggggtgc
ttggaggccacgacgtgcactggttctactcattgtcatcgcgcaactacgtgcacaaatagcaaagattgttttgaag
ctaccacatgtactgggtcatcaaactgctataccgctaccacctgcactaatagcacgaactgctataaagctactgc
atgtacaaatagtacggggtgcccagggcatcatcatcaccatcatcaccatcactagtactagagattaaagaggaga
atactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctcc
gtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcga
agtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttg
gcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcag
cgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttctt
ctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcga
aaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagtt
ctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgt
ccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacct
gctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatc
cgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggt
actacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaat
cctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatc
aacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgg
gcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacct
gtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaa
attgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtg
acgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaa
catccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatc
ggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtc
aggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaaga
taacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctat
gctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcg
ctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcga
cgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcacc
gtaatcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttg
aacagggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaata
ctagagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggt
atggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagca
catctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattg
ctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaa
agaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgtt
ctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaac
agacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatactt
ccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaa
taccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacc
tgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgct
ggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaa
atcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaacca
cggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggt
atctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtg
ccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcga
tcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctat
cgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacac
attccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctc
cgctggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagaaagaggagaaatactagagttagcctatg
gaagtcagggtgatcttaatccattaattaatgaaatcagcaaaatcatttcagcagcaggtagcttcgatgttaaaga
ggaaagaaccgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataaccctg
accacatcagcataataatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaat
tagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttaccct
taaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgc
ttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgca
ctatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtat
cgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaat
gtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccg
ttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtc
gacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctggg
attacacatggcatggatgagctctacaaataatactagagattaaagaggagaatactagatggattcctgtcacaag
attgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatc
gcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagca
agtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatc
ctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacaga
gcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaatt
cgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctg
caactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccc
tgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgc
gcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttc
gaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccc
tgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggt
gattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaa
tttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggttt
ctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaac
cattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatc
agcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctgg
cccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaag
ctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggat
tccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcg
gcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacct
ggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatc
atcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacg
atttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtca
gcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattac
gaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctga
gcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaacagggtaaacataaagagctgct
gtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactagagattaaagaggagaatacta
gatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgt
aagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccgg
tttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtca
ggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactcc
atcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcg
cagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccg
ttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagtt
ctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggaca
aaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcct
ggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaa
gctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtacc
agctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccct
ggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaa
gttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagtta
ccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgta
cactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtg
ttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcgg
ttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatc
tctgcacgaacgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata