LOCUS BBa_M1531 9258 bp DNA linear 22-Dec-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1531 VERSION BBa_M1531.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 229..414 /label=HlyA misc_feature 229..231 /label=Start misc_feature 229..414 /label=BBa_K208006 misc_feature 427..430 /label=conserved RBS 423..434 /label=BBa_B0034 CDS 441..917 /label=cds CDS 441..947 /label=BBa_M1524 RBS 956..969 /label=BBa_B0035 misc_feature 976..978 /label=Start Codon misc_feature 3097..3099 /label=Stop Codon CDS 976..3096 /label=HlyB CDS 976..3099 /label=BBa_M1003 RBS 3108..3121 /label=BBa_B0035 misc_feature 3128..3130 /label=Start Codon CDS 3128..4561 /label=HlyD misc_feature 4562..4564 /label=Stop Codon CDS 3128..4564 /label=BBa_M1004 misc_feature 956..4564 /label=BBa_M1528 misc_feature 4577..4580 /label=conserved RBS 4573..4584 /label=BBa_B0034 misc_feature 4593..4778 /label=HlyA misc_feature 4593..4595 /label=Start misc_feature 4593..4778 /label=BBa_K208006 misc_feature 4785..4787 /label=Start CDS 4785..5504 /label=GFP Coding Region CDS 4785..5504 /label=BBa_K208000 RBS 5513..5526 /label=BBa_B0035 misc_feature 5533..5535 /label=Start Codon misc_feature 7654..7656 /label=Stop Codon CDS 5533..7653 /label=HlyB CDS 5533..7656 /label=BBa_M1003 RBS 7665..7678 /label=BBa_B0035 misc_feature 7685..7687 /label=Start Codon CDS 7685..9118 /label=HlyD misc_feature 9119..9121 /label=Stop Codon CDS 7685..9121 /label=BBa_M1004 misc_feature 5513..9121 /label=BBa_M1528 stem_loop 9141..9184 /label=stem_loop misc_feature 9130..9209 /label=BBa_B0010 terminator 9130..9209 /label=BBa_B0010 stem_loop 9225..9244 /label=T7 TE misc_feature 9251..9251 /label=stop polyA_site 9245..9258 /label=polya misc_feature 9218..9258 /label=BBa_B0012 terminator 9218..9258 /label=BBa_B0012 terminator 9130..9258 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt agcctatgga 241 agtcagggtg atcttaatcc attaattaat gaaatcagca aaatcatttc agcagcaggt 301 agcttcgatg ttaaagagga aagaaccgca gcttctttat tgcagttgtc cggtaatgcc 361 agtgattttt catatggacg gaactcaata accctgacca catcagcata ataatactag 421 agaaagagga gaaatactag atggccttca aaacctgtgg tttttcaaaa aaatggttag 481 tcatagcagt cattgtcatg tgcctgtgta cggaatgtta ctgccagtgt acaggtggcg 541 ctgattgcac atcgtgtacc ggggcatgta ccgggtgtgg caattgtccg aacgcggtta 601 catgcacaaa ctcacagcat tgcgttaagg caaacacgtg cacagattct gagaattgcg 661 ttaaagctca tacatgcacc ggtagtaccg attgtaatac cgcgcagacc tgtactaaca 721 gcaaggattg cttcgaagcg aatacttgca ccggcagtac gcattgccat cgagctacta 781 cgtgtaccaa ttcgaaagat tgctttgagg caaccacatg caccggctcc tccaattgct 841 ataccgcgac cacctgcaca gatagcacta attgttacaa agctacggcg tgcacaaact 901 cttctggctg cccggggcat catcatcacc atcatcacca tcactagtac tagagattaa 961 agaggagaat actagatgga ttcctgtcac aagattgatt acggcctgta tgcactggaa 1021 atcctggcac aatatcataa cgtctccgtc aacccggagg aaattaaaca tcgcttcgat 1081 actgacggca ctggcctggg tctgaccagc tggctgctgg cagcgaagtc cctggagctg 1141 aaggtaaagc aagtgaaaaa gacgattgat cgtctgaact tcatcagcct gccggcactg 1201 gtttggcgtg aagatggccg tcacttcatc ctgacgaaag tgtccaaaga agcgaaccgc 1261 tacctgatct ttgatctgga gcagcgcaac ccgcgcgtac tggaacagag cgagttcgag 1321 gctctgtatc agggccacat cattctgatt gcatctcgtt cttctgttgc aggtaaactg 1381 gcgaaattcg acttcacctg gttcattccg gctattatca agtaccgtcg tattttcatc 1441 gaaaccctgg ttgtatctgt tttcctgcaa ctgttcgctc tgattactcc gctgttcttt 1501 caggttgtca tggacaaagt tctggttcac cgtggctttt ccaccctgaa cgtcattact 1561 gttgcgctga gcgttgttgt tgtgttcgaa atcattctgt ccggtctgcg tacctacatc 1621 ttcgcgcact ctacctcccg catcgacgtt gaactgggtg cgaagctgtt ccgtcacctg 1681 ctggcactgc ctatcagcta cttcgaatct cgtcgtgtgg gcgatactgt ggcccgtgtt 1741 cgtgaactgg atcagatccg caactttctg accggccagg ccctgaccag cgttctggat 1801 ctgctgttca gctttatctt cttcgcagta atgtggtact acagcccgaa gctgactctg 1861 gtgattctgt tctctctgcc gtgttacgca gcttggtccg tcttcatctc tccaatcctg 1921 cgtcgccgtc tggacgacaa atttagccgt aatgcggata atcagtcctt cctggtcgaa 1981 tctgtgactg ctatcaacac tatcaaagcc atggcggttt ctccacagat gactaacatc 2041 tgggataagc agctggcagg ttacgttgct gcgggcttca aagtgaccgt tctggcaacc 2101 attggtcaac agggcattca gctgattcag aaaaccgtga tgatcattaa cctgtggctg 2161 ggtgcgcacc tggtcatcag cggtgacctg tccattggcc agctgatcgc tttcaacatg 2221 ctggcgggtc aaattgttgc cccagtaatc cgtctggccc agatctggca ggacttccag 2281 caggtcggta tttctgtgac ccgcctgggt gacgtcctga actctccgac cgaaagctac 2341 cacggtaaac tggctctgcc ggagatcaac ggcgatatta ctttccgtaa catccgtttt 2401 cgttacaaac cggattcccc ggtaatcctg gataacatca atctgtctat caaacagggc 2461 gaagtcatcg gcatcgtggg ccgtagcggc agcggcaagt ccactctgac taaactgatt 2521 cagcgctttt acattccaga aaacggtcag gttctgattg acggtcacga cctggctctg 2581 gcggacccga actggctgcg tcgtcaggtg ggcgtagtgc tgcaagataa cgtgctgctg 2641 aaccgtagca tcatcgataa catcagcctg gctaacccgg gcatgtccgt ggaaaaagtt 2701 atctatgctg cgaaactggc aggcgcgcac gatttcatct ctgaactgcg tgaaggctac 2761 aacaccatcg taggcgaaca gggcgctggc ctgtccggcg gtcaacgtca gcgtatcgct 2821 attgcacgtg cactggtgaa caacccgaaa atcctgatct tcgacgaggc cacttctgct 2881 ctggattacg aatctgaaca tattatcatg cgtaatatgc acaagatctg caaaggtcgc 2941 accgtaatca tcatcgccca ccgtctgagc accgtaaaga acgccgaccg catcatcgtg 3001 atggaaaaag gtaagatcgt tgaacagggt aaacataaag agctgctgtc cgaaccggaa 3061 tctctgtatt cttatctgta ccagctgcaa tctgattaat actagagatt aaagaggaga 3121 atactagatg aaaacttggc tgatgggctt ctctgagttc ctgctgcgtt ataagctggt 3181 atggagcgaa acttggaaaa tccgtaagca actggatacc ccggtacgtg aaaaagacga 3241 aaacgaattt ctgccagcac atctggaact gattgagacg ccggtttctc gccgtccacg 3301 tctggttgct tacttcatta tgggcttcct ggttattgct gtaatcctgt ctgttctggg 3361 tcaggtggaa atcgttgcga ccgcgaatgg taaactgacc ctgtccggtc gttctaaaga 3421 aatcaaaccg atcgaaaact ccatcgttaa agagattatc gttaaagaag gtgaatccgt 3481 acgcaaaggc gatgttctgc tgaaactgac cgcactgggc gcagaagcgg acactctgaa 3541 aactcagtcc tctctgctgc aaacccgtct ggaacagacc cgttaccaaa tcctgagccg 3601 ttctattgaa ctgaacaaac tgccggaact gaaactgccg gatgaaccat acttccagaa 3661 cgtaagcgag gaggaagttc tgcgcctgac ttccctgatc aaagagcagt tctctacttg 3721 gcagaaccag aaataccaaa aggaactgaa cctggacaaa aagcgtgcgg agcgcctgac 3781 tatcctggcc cgcatcaacc gttacgagaa cctgtctcgt gttgaaaaat ctcgcctgga 3841 cgacttccgt agcctgctgc acaaacaggc catcgcaaaa cacgctgtgc tggaacagga 3901 aaataaatac gtggaagctg ccaacgaact gcgtgtatat aaaagccagc tggagcagat 3961 cgagagcgaa atcctgtctg cgaaggagga gtaccagctg gtgacgcagc tgttcaaaaa 4021 tgaaatcctg gacaaactgc gtcaaaccac ggactctatc gaactgctga ccctggaact 4081 ggagaaaaac gaggagcgcc agcaggccag cgtcatccgc gctccggtat ctggtaaagt 4141 tcagcaactg aaagttcaca ccgaaggtgg cgttgttacc accgcagaaa ccctgatggt 4201 tattgtgccg gaagatgaca ccctggaagt taccgctctg gtgcagaaca aagatatcgg 4261 tttcatcaac gtcggtcaga acgcgatcat caaagtagag gctttcccgt acactcgtta 4321 cggctacctg gtgggcaagg tcaaaaacat caacctggac gctatcgaag atcagaaact 4381 gggcctggtg ttcaacgtga tcgttagcgt agaagagaac gatctgtcta ccggtaacaa 4441 acacattcca ctgtcctccg gcatggcggt tactgctgaa atcaaaaccg gtatgcgtag 4501 cgtgatctcc tatctgctgt ctccgctgga agaaagcgtc accgaatctc tgcacgaacg 4561 ctaatactag agaaagagga gaaatactag agttagccta tggaagtcag ggtgatctta 4621 atccattaat taatgaaatc agcaaaatca tttcagcagc aggtagcttc gatgttaaag 4681 aggaaagaac cgcagcttct ttattgcagt tgtccggtaa tgccagtgat ttttcatatg 4741 gacggaactc aataaccctg accacatcag cataataata ctagatggct agcaaaggag 4801 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 4861 acaaattttc tgtcagtgga gagggtgaag gtgatgctac atacggaaag cttaccctta 4921 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct 4981 cttatggtgt tcaatgcttt tcccgttatc cggatcatat gaaacggcat gactttttca 5041 agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga 5101 actacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt 5161 taaaaggtat tgattttaaa gaagatggaa acattctcgg acacaaactc gagtacaact 5221 ataactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact 5281 tcaaaattcg ccacaacatt gaagatggat ccgttcaact agcagaccat tatcaacaaa 5341 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat 5401 ctgccctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa 5461 ctgctgctgg gattacacat ggcatggatg agctctacaa ataatactag agattaaaga 5521 ggagaatact agatggattc ctgtcacaag attgattacg gcctgtatgc actggaaatc 5581 ctggcacaat atcataacgt ctccgtcaac ccggaggaaa ttaaacatcg cttcgatact 5641 gacggcactg gcctgggtct gaccagctgg ctgctggcag cgaagtccct ggagctgaag 5701 gtaaagcaag tgaaaaagac gattgatcgt ctgaacttca tcagcctgcc ggcactggtt 5761 tggcgtgaag atggccgtca cttcatcctg acgaaagtgt ccaaagaagc gaaccgctac 5821 ctgatctttg atctggagca gcgcaacccg cgcgtactgg aacagagcga gttcgaggct 5881 ctgtatcagg gccacatcat tctgattgca tctcgttctt ctgttgcagg taaactggcg 5941 aaattcgact tcacctggtt cattccggct attatcaagt accgtcgtat tttcatcgaa 6001 accctggttg tatctgtttt cctgcaactg ttcgctctga ttactccgct gttctttcag 6061 gttgtcatgg acaaagttct ggttcaccgt ggcttttcca ccctgaacgt cattactgtt 6121 gcgctgagcg ttgttgttgt gttcgaaatc attctgtccg gtctgcgtac ctacatcttc 6181 gcgcactcta cctcccgcat cgacgttgaa ctgggtgcga agctgttccg tcacctgctg 6241 gcactgccta tcagctactt cgaatctcgt cgtgtgggcg atactgtggc ccgtgttcgt 6301 gaactggatc agatccgcaa ctttctgacc ggccaggccc tgaccagcgt tctggatctg 6361 ctgttcagct ttatcttctt cgcagtaatg tggtactaca gcccgaagct gactctggtg 6421 attctgttct ctctgccgtg ttacgcagct tggtccgtct tcatctctcc aatcctgcgt 6481 cgccgtctgg acgacaaatt tagccgtaat gcggataatc agtccttcct ggtcgaatct 6541 gtgactgcta tcaacactat caaagccatg gcggtttctc cacagatgac taacatctgg 6601 gataagcagc tggcaggtta cgttgctgcg ggcttcaaag tgaccgttct ggcaaccatt 6661 ggtcaacagg gcattcagct gattcagaaa accgtgatga tcattaacct gtggctgggt 6721 gcgcacctgg tcatcagcgg tgacctgtcc attggccagc tgatcgcttt caacatgctg 6781 gcgggtcaaa ttgttgcccc agtaatccgt ctggcccaga tctggcagga cttccagcag 6841 gtcggtattt ctgtgacccg cctgggtgac gtcctgaact ctccgaccga aagctaccac 6901 ggtaaactgg ctctgccgga gatcaacggc gatattactt tccgtaacat ccgttttcgt 6961 tacaaaccgg attccccggt aatcctggat aacatcaatc tgtctatcaa acagggcgaa 7021 gtcatcggca tcgtgggccg tagcggcagc ggcaagtcca ctctgactaa actgattcag 7081 cgcttttaca ttccagaaaa cggtcaggtt ctgattgacg gtcacgacct ggctctggcg 7141 gacccgaact ggctgcgtcg tcaggtgggc gtagtgctgc aagataacgt gctgctgaac 7201 cgtagcatca tcgataacat cagcctggct aacccgggca tgtccgtgga aaaagttatc 7261 tatgctgcga aactggcagg cgcgcacgat ttcatctctg aactgcgtga aggctacaac 7321 accatcgtag gcgaacaggg cgctggcctg tccggcggtc aacgtcagcg tatcgctatt 7381 gcacgtgcac tggtgaacaa cccgaaaatc ctgatcttcg acgaggccac ttctgctctg 7441 gattacgaat ctgaacatat tatcatgcgt aatatgcaca agatctgcaa aggtcgcacc 7501 gtaatcatca tcgcccaccg tctgagcacc gtaaagaacg ccgaccgcat catcgtgatg 7561 gaaaaaggta agatcgttga acagggtaaa cataaagagc tgctgtccga accggaatct 7621 ctgtattctt atctgtacca gctgcaatct gattaatact agagattaaa gaggagaata 7681 ctagatgaaa acttggctga tgggcttctc tgagttcctg ctgcgttata agctggtatg 7741 gagcgaaact tggaaaatcc gtaagcaact ggataccccg gtacgtgaaa aagacgaaaa 7801 cgaatttctg ccagcacatc tggaactgat tgagacgccg gtttctcgcc gtccacgtct 7861 ggttgcttac ttcattatgg gcttcctggt tattgctgta atcctgtctg ttctgggtca 7921 ggtggaaatc gttgcgaccg cgaatggtaa actgaccctg tccggtcgtt ctaaagaaat 7981 caaaccgatc gaaaactcca tcgttaaaga gattatcgtt aaagaaggtg aatccgtacg 8041 caaaggcgat gttctgctga aactgaccgc actgggcgca gaagcggaca ctctgaaaac 8101 tcagtcctct ctgctgcaaa cccgtctgga acagacccgt taccaaatcc tgagccgttc 8161 tattgaactg aacaaactgc cggaactgaa actgccggat gaaccatact tccagaacgt 8221 aagcgaggag gaagttctgc gcctgacttc cctgatcaaa gagcagttct ctacttggca 8281 gaaccagaaa taccaaaagg aactgaacct ggacaaaaag cgtgcggagc gcctgactat 8341 cctggcccgc atcaaccgtt acgagaacct gtctcgtgtt gaaaaatctc gcctggacga 8401 cttccgtagc ctgctgcaca aacaggccat cgcaaaacac gctgtgctgg aacaggaaaa 8461 taaatacgtg gaagctgcca acgaactgcg tgtatataaa agccagctgg agcagatcga 8521 gagcgaaatc ctgtctgcga aggaggagta ccagctggtg acgcagctgt tcaaaaatga 8581 aatcctggac aaactgcgtc aaaccacgga ctctatcgaa ctgctgaccc tggaactgga 8641 gaaaaacgag gagcgccagc aggccagcgt catccgcgct ccggtatctg gtaaagttca 8701 gcaactgaaa gttcacaccg aaggtggcgt tgttaccacc gcagaaaccc tgatggttat 8761 tgtgccggaa gatgacaccc tggaagttac cgctctggtg cagaacaaag atatcggttt 8821 catcaacgtc ggtcagaacg cgatcatcaa agtagaggct ttcccgtaca ctcgttacgg 8881 ctacctggtg ggcaaggtca aaaacatcaa cctggacgct atcgaagatc agaaactggg 8941 cctggtgttc aacgtgatcg ttagcgtaga agagaacgat ctgtctaccg gtaacaaaca 9001 cattccactg tcctccggca tggcggttac tgctgaaatc aaaaccggta tgcgtagcgt 9061 gatctcctat ctgctgtctc cgctggaaga aagcgtcacc gaatctctgc acgaacgcta 9121 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 9181 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 9241 ggcctttctg cgtttata //